Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying method
In this study, the probiotic Lactobacillus casei was microencapsulated using the method of spray-drying combined with polyelectrolyte complexation of alginate, fructooligosaccharide and chitosan, and cross-linking with calcium chloride, followed by freeze-drying. Survival rate and physicochemical pr...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Society of Chemists and Technologists of Macedonia
2012-06-01
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| Series: | Macedonian Journal of Chemistry and Chemical Engineering |
| Subjects: | |
| Online Access: | https://mjcce.org.mk/index.php/MJCCE/article/view/64 |
| _version_ | 1819315802890829824 |
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| author | Tanja Petreska Ivanovska Lidija Petruševska-Tozi Margita Dabevska Kostoska Nikola Geškovski Anita Grozdanov Chris Stain Trajče Stafilov Kristina Mladenovska |
| author_facet | Tanja Petreska Ivanovska Lidija Petruševska-Tozi Margita Dabevska Kostoska Nikola Geškovski Anita Grozdanov Chris Stain Trajče Stafilov Kristina Mladenovska |
| author_sort | Tanja Petreska Ivanovska |
| collection | DOAJ |
| description | In this study, the probiotic Lactobacillus casei was microencapsulated using the method of spray-drying combined with polyelectrolyte complexation of alginate, fructooligosaccharide and chitosan, and cross-linking with calcium chloride, followed by freeze-drying. Survival rate and physicochemical properties of the prepared microparticles were evaluated. In addition, viability of Lactobacillus casei in simulated gastric and intestinal juices was investigated. Positively charged microparticles with average size of 11.08±1.1 μm and high cell viability of 10.98±0.11 log cfu/g were prepared. The synbiotic microparticles were stable during exposure to simulated gastric and intestinal juices, while release of viable cells above the therapeutic value (8.31±0.14 log cfu/g) in the simulated colonic pH was observed.
The presented method for microencapsulation of synbiotics shows potential for effective protection of viable probiotic cells during exposure to harsh environmental conditions. |
| first_indexed | 2024-12-24T10:05:54Z |
| format | Article |
| id | doaj.art-a87727f25fcd4bb7b6c21b4b741b53f6 |
| institution | Directory Open Access Journal |
| issn | 1857-5552 1857-5625 |
| language | English |
| last_indexed | 2024-12-24T10:05:54Z |
| publishDate | 2012-06-01 |
| publisher | Society of Chemists and Technologists of Macedonia |
| record_format | Article |
| series | Macedonian Journal of Chemistry and Chemical Engineering |
| spelling | doaj.art-a87727f25fcd4bb7b6c21b4b741b53f62022-12-21T17:00:48ZengSociety of Chemists and Technologists of MacedoniaMacedonian Journal of Chemistry and Chemical Engineering1857-55521857-56252012-06-0131111512310.20450/mjcce.2012.6429Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying methodTanja Petreska Ivanovska0Lidija Petruševska-Tozi1Margita Dabevska Kostoska2Nikola Geškovski3Anita Grozdanov4Chris Stain5Trajče Stafilov6Kristina Mladenovska7Faculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 SkopjeFaculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 SkopjeCenter for Public Health – Tetovo, 29 Noemvri bb, TetovoFaculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 SkopjeFaculty of Technology and Metallurgy, Ss. Cyril and Methodius University, Rudjer Bošković 16, 1000 Skopjesity of Reading, Centre for Advanced Microscopy, Reading RG6 6AH, WhiteknightsInstitute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, SkopjeFaculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 SkopjeIn this study, the probiotic Lactobacillus casei was microencapsulated using the method of spray-drying combined with polyelectrolyte complexation of alginate, fructooligosaccharide and chitosan, and cross-linking with calcium chloride, followed by freeze-drying. Survival rate and physicochemical properties of the prepared microparticles were evaluated. In addition, viability of Lactobacillus casei in simulated gastric and intestinal juices was investigated. Positively charged microparticles with average size of 11.08±1.1 μm and high cell viability of 10.98±0.11 log cfu/g were prepared. The synbiotic microparticles were stable during exposure to simulated gastric and intestinal juices, while release of viable cells above the therapeutic value (8.31±0.14 log cfu/g) in the simulated colonic pH was observed. The presented method for microencapsulation of synbiotics shows potential for effective protection of viable probiotic cells during exposure to harsh environmental conditions.https://mjcce.org.mk/index.php/MJCCE/article/view/64lactobacillus caseichitosan-ca-alginate microparticlesspray-drying meth |
| spellingShingle | Tanja Petreska Ivanovska Lidija Petruševska-Tozi Margita Dabevska Kostoska Nikola Geškovski Anita Grozdanov Chris Stain Trajče Stafilov Kristina Mladenovska Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying method Macedonian Journal of Chemistry and Chemical Engineering lactobacillus casei chitosan-ca-alginate microparticles spray-drying meth |
| title | Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying method |
| title_full | Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying method |
| title_fullStr | Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying method |
| title_full_unstemmed | Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying method |
| title_short | Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying method |
| title_sort | microencapsulation of i lactobacillus casei i in chitosan ca alginate microparticles using spray drying method |
| topic | lactobacillus casei chitosan-ca-alginate microparticles spray-drying meth |
| url | https://mjcce.org.mk/index.php/MJCCE/article/view/64 |
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