Regulatory T Cells Contribute to the Inhibition of Radiation-Induced Acute Lung Inflammation via Bee Venom Phospholipase A2 in Mice
Bee venom has long been used to treat various inflammatory diseases, such as rheumatoid arthritis and multiple sclerosis. Previously, we reported that bee venom phospholipase A2 (bvPLA2) has an anti-inflammatory effect through the induction of regulatory T cells. Radiotherapy is a common anti-cancer...
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MDPI AG
2016-04-01
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| Online Access: | http://www.mdpi.com/2072-6651/8/5/131 |
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| author | Dasom Shin Gihyun Lee Sung-Hwa Sohn Soojin Park Kyung-Hwa Jung Ji Min Lee Jieun Yang Jaeho Cho Hyunsu Bae |
| author_facet | Dasom Shin Gihyun Lee Sung-Hwa Sohn Soojin Park Kyung-Hwa Jung Ji Min Lee Jieun Yang Jaeho Cho Hyunsu Bae |
| author_sort | Dasom Shin |
| collection | DOAJ |
| description | Bee venom has long been used to treat various inflammatory diseases, such as rheumatoid arthritis and multiple sclerosis. Previously, we reported that bee venom phospholipase A2 (bvPLA2) has an anti-inflammatory effect through the induction of regulatory T cells. Radiotherapy is a common anti-cancer method, but often causes adverse effects, such as inflammation. This study was conducted to evaluate the protective effects of bvPLA2 in radiation-induced acute lung inflammation. Mice were focally irradiated with 75 Gy of X-rays in the lung and administered bvPLA2 six times after radiation. To evaluate the level of inflammation, the number of immune cells, mRNA level of inflammatory cytokine, and histological changes in the lung were measured. BvPLA2 treatment reduced the accumulation of immune cells, such as macrophages, neutrophils, lymphocytes, and eosinophils. In addition, bvPLA2 treatment decreased inflammasome-, chemokine-, cytokine- and fibrosis-related genes’ mRNA expression. The histological results also demonstrated the attenuating effect of bvPLA2 on radiation-induced lung inflammation. Furthermore, regulatory T cell depletion abolished the therapeutic effects of bvPLA2 in radiation-induced pneumonitis, implicating the anti-inflammatory effects of bvPLA2 are dependent upon regulatory T cells. These results support the therapeutic potential of bvPLA2 in radiation pneumonitis and fibrosis treatments. |
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| language | English |
| last_indexed | 2024-04-11T18:25:34Z |
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| spelling | doaj.art-a87818832aac45bd827cc56038f193002022-12-22T04:09:39ZengMDPI AGToxins2072-66512016-04-018513110.3390/toxins8050131toxins8050131Regulatory T Cells Contribute to the Inhibition of Radiation-Induced Acute Lung Inflammation via Bee Venom Phospholipase A2 in MiceDasom Shin0Gihyun Lee1Sung-Hwa Sohn2Soojin Park3Kyung-Hwa Jung4Ji Min Lee5Jieun Yang6Jaeho Cho7Hyunsu Bae8Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South KoreaDepartment of Radiation Oncology, Yonsei University College of Medicine, Seoul 120-752, South KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South KoreaDepartment of Radiation Oncology, Yonsei University College of Medicine, Seoul 120-752, South KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South KoreaDepartment of Radiation Oncology, Yonsei University College of Medicine, Seoul 120-752, South KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South KoreaBee venom has long been used to treat various inflammatory diseases, such as rheumatoid arthritis and multiple sclerosis. Previously, we reported that bee venom phospholipase A2 (bvPLA2) has an anti-inflammatory effect through the induction of regulatory T cells. Radiotherapy is a common anti-cancer method, but often causes adverse effects, such as inflammation. This study was conducted to evaluate the protective effects of bvPLA2 in radiation-induced acute lung inflammation. Mice were focally irradiated with 75 Gy of X-rays in the lung and administered bvPLA2 six times after radiation. To evaluate the level of inflammation, the number of immune cells, mRNA level of inflammatory cytokine, and histological changes in the lung were measured. BvPLA2 treatment reduced the accumulation of immune cells, such as macrophages, neutrophils, lymphocytes, and eosinophils. In addition, bvPLA2 treatment decreased inflammasome-, chemokine-, cytokine- and fibrosis-related genes’ mRNA expression. The histological results also demonstrated the attenuating effect of bvPLA2 on radiation-induced lung inflammation. Furthermore, regulatory T cell depletion abolished the therapeutic effects of bvPLA2 in radiation-induced pneumonitis, implicating the anti-inflammatory effects of bvPLA2 are dependent upon regulatory T cells. These results support the therapeutic potential of bvPLA2 in radiation pneumonitis and fibrosis treatments.http://www.mdpi.com/2072-6651/8/5/131bee venomphospholipase A2inflammationradiotherapyregulatory T cells |
| spellingShingle | Dasom Shin Gihyun Lee Sung-Hwa Sohn Soojin Park Kyung-Hwa Jung Ji Min Lee Jieun Yang Jaeho Cho Hyunsu Bae Regulatory T Cells Contribute to the Inhibition of Radiation-Induced Acute Lung Inflammation via Bee Venom Phospholipase A2 in Mice Toxins bee venom phospholipase A2 inflammation radiotherapy regulatory T cells |
| title | Regulatory T Cells Contribute to the Inhibition of Radiation-Induced Acute Lung Inflammation via Bee Venom Phospholipase A2 in Mice |
| title_full | Regulatory T Cells Contribute to the Inhibition of Radiation-Induced Acute Lung Inflammation via Bee Venom Phospholipase A2 in Mice |
| title_fullStr | Regulatory T Cells Contribute to the Inhibition of Radiation-Induced Acute Lung Inflammation via Bee Venom Phospholipase A2 in Mice |
| title_full_unstemmed | Regulatory T Cells Contribute to the Inhibition of Radiation-Induced Acute Lung Inflammation via Bee Venom Phospholipase A2 in Mice |
| title_short | Regulatory T Cells Contribute to the Inhibition of Radiation-Induced Acute Lung Inflammation via Bee Venom Phospholipase A2 in Mice |
| title_sort | regulatory t cells contribute to the inhibition of radiation induced acute lung inflammation via bee venom phospholipase a2 in mice |
| topic | bee venom phospholipase A2 inflammation radiotherapy regulatory T cells |
| url | http://www.mdpi.com/2072-6651/8/5/131 |
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