Biogenic Synthesis of Silver Nanoparticles Using <i>Catharanthus roseus</i> and Its Cytotoxicity Effect on Vero Cell Lines
<b>Background</b>: Type 2 diabetes mellitus (DM2) is a chronic and sometimes fatal condition which affects people all over the world. Nanotherapeutics have shown tremendous potential to combat chronic diseases—including DM2—as they enhance the overall impact of drugs on biological system...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/19/6191 |
| _version_ | 1797478071267229696 |
|---|---|
| author | Khansa Jamil Sahir Hameed Khattak Anum Farrukh Sania Begum Muhammad Naeem Riaz Aish Muhammad Tahira Kamal Touqeer Taj Imran Khan Sundus Riaz Huma Batool Kaleemullah Mandokhail Sabahat Majeed Sajid Ali Khan Bangash Alia Mushtaq Shahab Bashir Imdad Kaleem Fahed Pervaiz Aamir Rasool Muhammad Ammar Amanat Ghulam Muhammad Ali |
| author_facet | Khansa Jamil Sahir Hameed Khattak Anum Farrukh Sania Begum Muhammad Naeem Riaz Aish Muhammad Tahira Kamal Touqeer Taj Imran Khan Sundus Riaz Huma Batool Kaleemullah Mandokhail Sabahat Majeed Sajid Ali Khan Bangash Alia Mushtaq Shahab Bashir Imdad Kaleem Fahed Pervaiz Aamir Rasool Muhammad Ammar Amanat Ghulam Muhammad Ali |
| author_sort | Khansa Jamil |
| collection | DOAJ |
| description | <b>Background</b>: Type 2 diabetes mellitus (DM2) is a chronic and sometimes fatal condition which affects people all over the world. Nanotherapeutics have shown tremendous potential to combat chronic diseases—including DM2—as they enhance the overall impact of drugs on biological systems. Greenly synthesized silver nanoparticles (AgNPs) from <i>Catharanthus roseus</i> methanolic extract (<i>C.</i> AgNPs) were examined primarily for their cytotoxic and antidiabetic effects. <b>Methods</b>: Characterization of <i>C.</i> AgNPs was performed by UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and atomic force microscopy (AFM). The <i>C.</i> AgNPs were trialed on Vero cell line and afterwards on an animal model (rats). <b>Results</b>: The <i>C.</i> AgNPs showed standard structural and functional characterization as revealed by FTIR and XRD analyses. The zetapotential analysis indicated stability while EDX analysis confirmed the formation of composite capping with Ag metal. The cytotoxic effect (IC50) of <i>C.</i> AgNPs on Vero cell lines was found to be 568 g/mL. The animal model analyses further revealed a significant difference in water intake, food intake, body weight, urine volume, and urine sugar of tested rats after treatment with aqueous extract of <i>C.</i> AgNPs. Moreover, five groups of rats including control and diabetic groups (NC1, PC2, DG1, DG2, and DG3) were investigated for their blood glucose and glycemic control analysis. Conclusions: The <i>C.</i> AgNPs exhibited positive potential on the Vero cell line as well as on experimental rats. The lipid profile in all the diabetic groups (DG1-3) were significantly increased compared with both of the control groups (<i>p</i> < 0.05). The present study revealed the significance of <i>C.</i> AgNPs in nanotherapeutics. |
| first_indexed | 2024-03-09T21:26:49Z |
| format | Article |
| id | doaj.art-a87d08a8e06c459bb27f2485a849e7dc |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:26:49Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a87d08a8e06c459bb27f2485a849e7dc2023-11-23T21:07:44ZengMDPI AGMolecules1420-30492022-09-012719619110.3390/molecules27196191Biogenic Synthesis of Silver Nanoparticles Using <i>Catharanthus roseus</i> and Its Cytotoxicity Effect on Vero Cell LinesKhansa Jamil0Sahir Hameed Khattak1Anum Farrukh2Sania Begum3Muhammad Naeem Riaz4Aish Muhammad5Tahira Kamal6Touqeer Taj7Imran Khan8Sundus Riaz9Huma Batool10Kaleemullah Mandokhail11Sabahat Majeed12Sajid Ali Khan Bangash13Alia Mushtaq14Shahab Bashir15Imdad Kaleem16Fahed Pervaiz17Aamir Rasool18Muhammad Ammar Amanat19Ghulam Muhammad Ali20National Institute for Genomics and Advanced Biotechnology (N.I.G.A.B.), National Agriculture Research Centre (NARC), Islamabad 44000, PakistanNational Institute for Genomics and Advanced Biotechnology (N.I.G.A.B.), National Agriculture Research Centre (NARC), Islamabad 44000, PakistanDepartment of General Medicine, Fauji Foundation Hospital, Rawalpindi 45000, PakistanNational Institute for Genomics and Advanced Biotechnology (N.I.G.A.B.), National Agriculture Research Centre (NARC), Islamabad 44000, PakistanNational Institute for Genomics and Advanced Biotechnology (N.I.G.A.B.), National Agriculture Research Centre (NARC), Islamabad 44000, PakistanNational Institute for Genomics and Advanced Biotechnology (N.I.G.A.B.), National Agriculture Research Centre (NARC), Islamabad 44000, PakistanNational Institute for Genomics and Advanced Biotechnology (N.I.G.A.B.), National Agriculture Research Centre (NARC), Islamabad 44000, PakistanEcotoxicology Department, National Agriculture Research Centre (NARC), Islamabad 44000, PakistanNational Institute for Genomics and Advanced Biotechnology (N.I.G.A.B.), National Agriculture Research Centre (NARC), Islamabad 44000, PakistanFood Sciences Research Institute, National Agriculture Research Centre (NARC), Islamabad 44000, PakistanDepartment of Botany, Sardar Bahadur Khan’s Women’s University, Quetta 87300, PakistanDepartment of Microbiology, Balochistan University, Quetta 87300, PakistanDepartment of Biosciences, COMSATS University, Islamabad 45550, PakistanInstitute of Biotechnology and Genetic Engineering (IBGE), Khyber Pakhtunkhwa Agriculture University, Peshawar 25130, PakistanCrop Disease Research Institute CDRI, National Agriculture Research Centre, Islamabad 44000, PakistanDepartment of Biosciences, COMSATS University, Islamabad 45550, PakistanDepartment of Biosciences, COMSATS University, Islamabad 45550, PakistanDepartment of Biosciences, COMSATS University, Islamabad 45550, PakistanInstitute of Biochemistry, Balochistan University, Quetta 08770, PakistanNational Institute for Genomics and Advanced Biotechnology (N.I.G.A.B.), National Agriculture Research Centre (NARC), Islamabad 44000, PakistanNational Institute for Genomics and Advanced Biotechnology (N.I.G.A.B.), National Agriculture Research Centre (NARC), Islamabad 44000, Pakistan<b>Background</b>: Type 2 diabetes mellitus (DM2) is a chronic and sometimes fatal condition which affects people all over the world. Nanotherapeutics have shown tremendous potential to combat chronic diseases—including DM2—as they enhance the overall impact of drugs on biological systems. Greenly synthesized silver nanoparticles (AgNPs) from <i>Catharanthus roseus</i> methanolic extract (<i>C.</i> AgNPs) were examined primarily for their cytotoxic and antidiabetic effects. <b>Methods</b>: Characterization of <i>C.</i> AgNPs was performed by UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and atomic force microscopy (AFM). The <i>C.</i> AgNPs were trialed on Vero cell line and afterwards on an animal model (rats). <b>Results</b>: The <i>C.</i> AgNPs showed standard structural and functional characterization as revealed by FTIR and XRD analyses. The zetapotential analysis indicated stability while EDX analysis confirmed the formation of composite capping with Ag metal. The cytotoxic effect (IC50) of <i>C.</i> AgNPs on Vero cell lines was found to be 568 g/mL. The animal model analyses further revealed a significant difference in water intake, food intake, body weight, urine volume, and urine sugar of tested rats after treatment with aqueous extract of <i>C.</i> AgNPs. Moreover, five groups of rats including control and diabetic groups (NC1, PC2, DG1, DG2, and DG3) were investigated for their blood glucose and glycemic control analysis. Conclusions: The <i>C.</i> AgNPs exhibited positive potential on the Vero cell line as well as on experimental rats. The lipid profile in all the diabetic groups (DG1-3) were significantly increased compared with both of the control groups (<i>p</i> < 0.05). The present study revealed the significance of <i>C.</i> AgNPs in nanotherapeutics.https://www.mdpi.com/1420-3049/27/19/6191nanoparticles (NPs)silver nanoparticles (AgNPs)<i>C. roseus</i>Vero cell lineanimal modelantidiabetic potential |
| spellingShingle | Khansa Jamil Sahir Hameed Khattak Anum Farrukh Sania Begum Muhammad Naeem Riaz Aish Muhammad Tahira Kamal Touqeer Taj Imran Khan Sundus Riaz Huma Batool Kaleemullah Mandokhail Sabahat Majeed Sajid Ali Khan Bangash Alia Mushtaq Shahab Bashir Imdad Kaleem Fahed Pervaiz Aamir Rasool Muhammad Ammar Amanat Ghulam Muhammad Ali Biogenic Synthesis of Silver Nanoparticles Using <i>Catharanthus roseus</i> and Its Cytotoxicity Effect on Vero Cell Lines Molecules nanoparticles (NPs) silver nanoparticles (AgNPs) <i>C. roseus</i> Vero cell line animal model antidiabetic potential |
| title | Biogenic Synthesis of Silver Nanoparticles Using <i>Catharanthus roseus</i> and Its Cytotoxicity Effect on Vero Cell Lines |
| title_full | Biogenic Synthesis of Silver Nanoparticles Using <i>Catharanthus roseus</i> and Its Cytotoxicity Effect on Vero Cell Lines |
| title_fullStr | Biogenic Synthesis of Silver Nanoparticles Using <i>Catharanthus roseus</i> and Its Cytotoxicity Effect on Vero Cell Lines |
| title_full_unstemmed | Biogenic Synthesis of Silver Nanoparticles Using <i>Catharanthus roseus</i> and Its Cytotoxicity Effect on Vero Cell Lines |
| title_short | Biogenic Synthesis of Silver Nanoparticles Using <i>Catharanthus roseus</i> and Its Cytotoxicity Effect on Vero Cell Lines |
| title_sort | biogenic synthesis of silver nanoparticles using i catharanthus roseus i and its cytotoxicity effect on vero cell lines |
| topic | nanoparticles (NPs) silver nanoparticles (AgNPs) <i>C. roseus</i> Vero cell line animal model antidiabetic potential |
| url | https://www.mdpi.com/1420-3049/27/19/6191 |
| work_keys_str_mv | AT khansajamil biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT sahirhameedkhattak biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT anumfarrukh biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT saniabegum biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT muhammadnaeemriaz biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT aishmuhammad biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT tahirakamal biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT touqeertaj biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT imrankhan biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT sundusriaz biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT humabatool biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT kaleemullahmandokhail biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT sabahatmajeed biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT sajidalikhanbangash biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT aliamushtaq biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT shahabbashir biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT imdadkaleem biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT fahedpervaiz biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT aamirrasool biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT muhammadammaramanat biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines AT ghulammuhammadali biogenicsynthesisofsilvernanoparticlesusingicatharanthusroseusianditscytotoxicityeffectonverocelllines |