Isolation and identification of obligately chemolithoautotrophic, haloalkaliphilic bacterium Thioalkalivibrio sp. strain EMA and optimizing its thiosulfate removal activity in haloalkaliphilic condition
Introduction: Dischargeing hazardous pollutants of oil and gas industries such as spent caustic into the soil and water is an environmental concern for which biological treatment could offer a solution. To remove high levels of sulfur compounds in spent caustic waste, isolation of chemolithoautotrop...
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| Format: | Article |
| Language: | English |
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University of Isfahan
2017-03-01
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| Series: | Biological Journal of Microorganism |
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| Online Access: | http://bjm.ui.ac.ir/article_21308_ffe554e0a7a9fb199b2c66206f4cc417.pdf |
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| author | Somaye Makzum Mohammad Ali Amoozegar Seyed Mohammad Mehdi Dastgheib |
| author_facet | Somaye Makzum Mohammad Ali Amoozegar Seyed Mohammad Mehdi Dastgheib |
| author_sort | Somaye Makzum |
| collection | DOAJ |
| description | Introduction: Dischargeing hazardous pollutants of oil and gas industries such as spent caustic into the soil and water is an environmental concern for which biological treatment could offer a solution. To remove high levels of sulfur compounds in spent caustic waste, isolation of chemolithoautotrophic sulfur-oxidizing bacteria from Meighan wetland was considered in this study.
Materials and methods: For isolation of chemolithoautotroph haloalkaliphile sulfur-oxidizing bacteria, alkaline sulfur-respiring medium with sodium thiosulfate as the sole electron and energy source and sodium carbonate/bicarbonate as carbon source were utilized. To confirm that the purified isolates are obligated autotroph, their growth on nutrient agar medium was surveyed and selected strains were identified based on 16S rRNA sequence analysis. The growth and activity of selected strain named EMA were optimized at various pHs and salt conditions by assessment of protein content and remained thiosulfate in the culture medium.
Results: Following enrichment, 10 chemolithoautotrophic, haloalkaliphilic sulfur-oxidizing strains were isolated. 16S rRNA sequence analysis, showed that the strains belonged to the genus Thioalkalivibrio. The optimal growth and thiosulfate removal of the selected strain were obtained at pH 10 and 50 g/l of salt (sodium chloride or sodium sulfate). At higher salt concentrations, thiosulfate removal was higher in the presence of sodium sulfate, rather than sodium chloride.
Discussion and conclusion: high sulfur removal activity of the isolated haloalkaliphilic Thioalkalivibrio strains in extreme conditions with these bacteria could be promising for the biotreatment of spent caustic. |
| first_indexed | 2024-04-12T11:45:21Z |
| format | Article |
| id | doaj.art-a8819bf963694d818ff247f90d644c28 |
| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-04-12T11:45:21Z |
| publishDate | 2017-03-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-a8819bf963694d818ff247f90d644c282022-12-22T03:34:22ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812017-03-01621152921308Isolation and identification of obligately chemolithoautotrophic, haloalkaliphilic bacterium Thioalkalivibrio sp. strain EMA and optimizing its thiosulfate removal activity in haloalkaliphilic conditionSomaye Makzum0Mohammad Ali Amoozegar1Seyed Mohammad Mehdi Dastgheib2M.Sc. of Biotechnology, University of Tehran, Tehran, IranProfessor of Microbiology, University of Tehran, Tehran, IranAssistant Professor of Biotechnology, Research institute of petroleum industry (RIPI), Tehran, IranIntroduction: Dischargeing hazardous pollutants of oil and gas industries such as spent caustic into the soil and water is an environmental concern for which biological treatment could offer a solution. To remove high levels of sulfur compounds in spent caustic waste, isolation of chemolithoautotrophic sulfur-oxidizing bacteria from Meighan wetland was considered in this study. Materials and methods: For isolation of chemolithoautotroph haloalkaliphile sulfur-oxidizing bacteria, alkaline sulfur-respiring medium with sodium thiosulfate as the sole electron and energy source and sodium carbonate/bicarbonate as carbon source were utilized. To confirm that the purified isolates are obligated autotroph, their growth on nutrient agar medium was surveyed and selected strains were identified based on 16S rRNA sequence analysis. The growth and activity of selected strain named EMA were optimized at various pHs and salt conditions by assessment of protein content and remained thiosulfate in the culture medium. Results: Following enrichment, 10 chemolithoautotrophic, haloalkaliphilic sulfur-oxidizing strains were isolated. 16S rRNA sequence analysis, showed that the strains belonged to the genus Thioalkalivibrio. The optimal growth and thiosulfate removal of the selected strain were obtained at pH 10 and 50 g/l of salt (sodium chloride or sodium sulfate). At higher salt concentrations, thiosulfate removal was higher in the presence of sodium sulfate, rather than sodium chloride. Discussion and conclusion: high sulfur removal activity of the isolated haloalkaliphilic Thioalkalivibrio strains in extreme conditions with these bacteria could be promising for the biotreatment of spent caustic.http://bjm.ui.ac.ir/article_21308_ffe554e0a7a9fb199b2c66206f4cc417.pdfSpent causticOptimizationBiological treatmentChemolithoautotrophesulfur-oxidizing bacteria |
| spellingShingle | Somaye Makzum Mohammad Ali Amoozegar Seyed Mohammad Mehdi Dastgheib Isolation and identification of obligately chemolithoautotrophic, haloalkaliphilic bacterium Thioalkalivibrio sp. strain EMA and optimizing its thiosulfate removal activity in haloalkaliphilic condition Biological Journal of Microorganism Spent caustic Optimization Biological treatment Chemolithoautotrophe sulfur-oxidizing bacteria |
| title | Isolation and identification of obligately chemolithoautotrophic, haloalkaliphilic bacterium Thioalkalivibrio sp. strain EMA and optimizing its thiosulfate removal activity in haloalkaliphilic condition |
| title_full | Isolation and identification of obligately chemolithoautotrophic, haloalkaliphilic bacterium Thioalkalivibrio sp. strain EMA and optimizing its thiosulfate removal activity in haloalkaliphilic condition |
| title_fullStr | Isolation and identification of obligately chemolithoautotrophic, haloalkaliphilic bacterium Thioalkalivibrio sp. strain EMA and optimizing its thiosulfate removal activity in haloalkaliphilic condition |
| title_full_unstemmed | Isolation and identification of obligately chemolithoautotrophic, haloalkaliphilic bacterium Thioalkalivibrio sp. strain EMA and optimizing its thiosulfate removal activity in haloalkaliphilic condition |
| title_short | Isolation and identification of obligately chemolithoautotrophic, haloalkaliphilic bacterium Thioalkalivibrio sp. strain EMA and optimizing its thiosulfate removal activity in haloalkaliphilic condition |
| title_sort | isolation and identification of obligately chemolithoautotrophic haloalkaliphilic bacterium thioalkalivibrio sp strain ema and optimizing its thiosulfate removal activity in haloalkaliphilic condition |
| topic | Spent caustic Optimization Biological treatment Chemolithoautotrophe sulfur-oxidizing bacteria |
| url | http://bjm.ui.ac.ir/article_21308_ffe554e0a7a9fb199b2c66206f4cc417.pdf |
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