Inhibition of Tomato Mosaic Virus replication in tomato plant Lycopersicon esculentum by Trichoderma harzianum mill fungi and its supernatant which filteration with chlorophorm.
The study was conducted to know the effect of crud filtrate of T. harzianum and its supernatant which screened with chlorophorm in replication of Tomato mosaic virus in tomato plant Lycopersicon esculentum and relied Datura stramonium plants as indicator plant to determind the number of local lesion...
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| Format: | Article |
| Language: | English |
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University of Anbar
2015-12-01
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| Series: | مجلة جامعة الانبار للعلوم الصرفة |
| Subjects: | |
| Online Access: | https://juaps.uoanbar.edu.iq/article_127625_2102d9f5f1d2912ddf146b0bd4308be8.pdf |
| _version_ | 1827396189588291584 |
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| author | Rajaa fadhil hamdi Sajid Salahuddin Saleem |
| author_facet | Rajaa fadhil hamdi Sajid Salahuddin Saleem |
| author_sort | Rajaa fadhil hamdi |
| collection | DOAJ |
| description | The study was conducted to know the effect of crud filtrate of T. harzianum and its supernatant which screened with chlorophorm in replication of Tomato mosaic virus in tomato plant Lycopersicon esculentum and relied Datura stramonium plants as indicator plant to determind the number of local lesion, the results showed the positive effect of protein sediments in reduce the number of local lesion in Datura plants which which gave 3.145 lession/ cm2 while supernatant gave 5.236 lession/ cm2 and phosphate buffer treatment gave 7.176 lession/ cm2 .The results showed that the treatment of sidements proteins from supernatant which filtrate with chlorophorm reduced the number of local lesion with rate 5.295 lession/ cm2 compared with two control treatment ( water, potato media) which gave 8.212, 8.333 lession/ cm2 respectivly, while the crud filtrate treatment gave rate 5.012 lession/ cm2 , that mean, the treatment of crud filtrate succese to reduce the number of lesion and reduced the virus replication comparison with protein extract. Also, boiling the crud filtrate and protein extract led to losing the activity of both treatments, because the protein substances lost there inhibition activity when they exposed to high heat degree.The concentration 10-1 for crud filtrate and its filtrate screening with chlorophorm gave the grate effect in inhibition the virus in cm2 then the concentration 10-2 then 10-3 . |
| first_indexed | 2024-03-08T18:47:13Z |
| format | Article |
| id | doaj.art-a8820cd659cc4dd5ab06aa6175027196 |
| institution | Directory Open Access Journal |
| issn | 1991-8941 2706-6703 |
| language | English |
| last_indexed | 2024-03-08T18:47:13Z |
| publishDate | 2015-12-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة جامعة الانبار للعلوم الصرفة |
| spelling | doaj.art-a8820cd659cc4dd5ab06aa61750271962023-12-28T21:58:13ZengUniversity of Anbarمجلة جامعة الانبار للعلوم الصرفة1991-89412706-67032015-12-019313614310.37652/juaps.2015.127625127625Inhibition of Tomato Mosaic Virus replication in tomato plant Lycopersicon esculentum by Trichoderma harzianum mill fungi and its supernatant which filteration with chlorophorm.Rajaa fadhil hamdi0Sajid Salahuddin Saleem1University of Anbar / College of ScienceUniversity of Anbar / College of ScienceThe study was conducted to know the effect of crud filtrate of T. harzianum and its supernatant which screened with chlorophorm in replication of Tomato mosaic virus in tomato plant Lycopersicon esculentum and relied Datura stramonium plants as indicator plant to determind the number of local lesion, the results showed the positive effect of protein sediments in reduce the number of local lesion in Datura plants which which gave 3.145 lession/ cm2 while supernatant gave 5.236 lession/ cm2 and phosphate buffer treatment gave 7.176 lession/ cm2 .The results showed that the treatment of sidements proteins from supernatant which filtrate with chlorophorm reduced the number of local lesion with rate 5.295 lession/ cm2 compared with two control treatment ( water, potato media) which gave 8.212, 8.333 lession/ cm2 respectivly, while the crud filtrate treatment gave rate 5.012 lession/ cm2 , that mean, the treatment of crud filtrate succese to reduce the number of lesion and reduced the virus replication comparison with protein extract. Also, boiling the crud filtrate and protein extract led to losing the activity of both treatments, because the protein substances lost there inhibition activity when they exposed to high heat degree.The concentration 10-1 for crud filtrate and its filtrate screening with chlorophorm gave the grate effect in inhibition the virus in cm2 then the concentration 10-2 then 10-3 .https://juaps.uoanbar.edu.iq/article_127625_2102d9f5f1d2912ddf146b0bd4308be8.pdftrichoderma harzianum |
| spellingShingle | Rajaa fadhil hamdi Sajid Salahuddin Saleem Inhibition of Tomato Mosaic Virus replication in tomato plant Lycopersicon esculentum by Trichoderma harzianum mill fungi and its supernatant which filteration with chlorophorm. مجلة جامعة الانبار للعلوم الصرفة trichoderma harzianum |
| title | Inhibition of Tomato Mosaic Virus replication in tomato plant Lycopersicon esculentum by Trichoderma harzianum mill fungi and its supernatant which filteration with chlorophorm. |
| title_full | Inhibition of Tomato Mosaic Virus replication in tomato plant Lycopersicon esculentum by Trichoderma harzianum mill fungi and its supernatant which filteration with chlorophorm. |
| title_fullStr | Inhibition of Tomato Mosaic Virus replication in tomato plant Lycopersicon esculentum by Trichoderma harzianum mill fungi and its supernatant which filteration with chlorophorm. |
| title_full_unstemmed | Inhibition of Tomato Mosaic Virus replication in tomato plant Lycopersicon esculentum by Trichoderma harzianum mill fungi and its supernatant which filteration with chlorophorm. |
| title_short | Inhibition of Tomato Mosaic Virus replication in tomato plant Lycopersicon esculentum by Trichoderma harzianum mill fungi and its supernatant which filteration with chlorophorm. |
| title_sort | inhibition of tomato mosaic virus replication in tomato plant lycopersicon esculentum by trichoderma harzianum mill fungi and its supernatant which filteration with chlorophorm |
| topic | trichoderma harzianum |
| url | https://juaps.uoanbar.edu.iq/article_127625_2102d9f5f1d2912ddf146b0bd4308be8.pdf |
| work_keys_str_mv | AT rajaafadhilhamdi inhibitionoftomatomosaicvirusreplicationintomatoplantlycopersiconesculentumbytrichodermaharzianummillfungianditssupernatantwhichfilterationwithchlorophorm AT sajidsalahuddinsaleem inhibitionoftomatomosaicvirusreplicationintomatoplantlycopersiconesculentumbytrichodermaharzianummillfungianditssupernatantwhichfilterationwithchlorophorm |