Flexible, AAV-equipped Genetic Modules for Inducible Control of Gene Expression in Mammalian Brain
Controlling gene expression in mammalian brain is of utmost importance to causally link the role of gene function to cell circuit dynamics under normal conditions and disease states. We have developed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches for ind...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-01-01
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| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253117300379 |
| _version_ | 1819068748904005632 |
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| author | Godwin K Dogbevia Martin Roβmanith Rolf Sprengel Mazahir T Hasan |
| author_facet | Godwin K Dogbevia Martin Roβmanith Rolf Sprengel Mazahir T Hasan |
| author_sort | Godwin K Dogbevia |
| collection | DOAJ |
| description | Controlling gene expression in mammalian brain is of utmost importance to causally link the role of gene function to cell circuit dynamics under normal conditions and disease states. We have developed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches for inducible and reversible control of gene expression in a cell type specific and brain subregion selective manner. Here, we characterize a two-virus approach to efficiently and reliably switch gene expression on and off, repetitively, both in vitro and in vivo. Our recombinant adeno-associated virus (rAAV)-Tet approach is highly flexible and it has great potential for application in basic and biomedical neuroscience research and gene therapy. |
| first_indexed | 2024-12-21T16:39:05Z |
| format | Article |
| id | doaj.art-a882159a44d54a1d938eb033a970d124 |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-12-21T16:39:05Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-a882159a44d54a1d938eb033a970d1242022-12-21T18:57:08ZengElsevierMolecular Therapy: Nucleic Acids2162-25312016-01-015C10.1038/mtna.2016.23Flexible, AAV-equipped Genetic Modules for Inducible Control of Gene Expression in Mammalian BrainGodwin K Dogbevia0Martin Roβmanith1Rolf Sprengel2Mazahir T Hasan3Department of Neurobiology, Max Planck Institute for Medical Research, Heidelberg, GermanyDepartment of Neurobiology, Max Planck Institute for Medical Research, Heidelberg, GermanyMax Planck Research Group at the Institute for Anatomy and Cell Biology, Heidelberg University, Heidelberg, GermanyDepartment of Neurobiology, Max Planck Institute for Medical Research, Heidelberg, GermanyControlling gene expression in mammalian brain is of utmost importance to causally link the role of gene function to cell circuit dynamics under normal conditions and disease states. We have developed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches for inducible and reversible control of gene expression in a cell type specific and brain subregion selective manner. Here, we characterize a two-virus approach to efficiently and reliably switch gene expression on and off, repetitively, both in vitro and in vivo. Our recombinant adeno-associated virus (rAAV)-Tet approach is highly flexible and it has great potential for application in basic and biomedical neuroscience research and gene therapy.http://www.sciencedirect.com/science/article/pii/S2162253117300379braininducible and reversible gene expressionTet systemsrecombinant adeno-associated virus |
| spellingShingle | Godwin K Dogbevia Martin Roβmanith Rolf Sprengel Mazahir T Hasan Flexible, AAV-equipped Genetic Modules for Inducible Control of Gene Expression in Mammalian Brain Molecular Therapy: Nucleic Acids brain inducible and reversible gene expression Tet systems recombinant adeno-associated virus |
| title | Flexible, AAV-equipped Genetic Modules for Inducible Control of Gene Expression in Mammalian Brain |
| title_full | Flexible, AAV-equipped Genetic Modules for Inducible Control of Gene Expression in Mammalian Brain |
| title_fullStr | Flexible, AAV-equipped Genetic Modules for Inducible Control of Gene Expression in Mammalian Brain |
| title_full_unstemmed | Flexible, AAV-equipped Genetic Modules for Inducible Control of Gene Expression in Mammalian Brain |
| title_short | Flexible, AAV-equipped Genetic Modules for Inducible Control of Gene Expression in Mammalian Brain |
| title_sort | flexible aav equipped genetic modules for inducible control of gene expression in mammalian brain |
| topic | brain inducible and reversible gene expression Tet systems recombinant adeno-associated virus |
| url | http://www.sciencedirect.com/science/article/pii/S2162253117300379 |
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