Neurocalcin regulates nighttime sleep and arousal in Drosophila
Sleep-like states in diverse organisms can be separated into distinct stages, each with a characteristic arousal threshold. However, the molecular pathways underlying different sleep stages remain unclear. The fruit fly, Drosophila melanogaster, exhibits consolidated sleep during both day and night,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/38114 |
| _version_ | 1811201076368506880 |
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| author | Ko-Fan Chen Simon Lowe Angélique Lamaze Patrick Krätschmer James Jepson |
| author_facet | Ko-Fan Chen Simon Lowe Angélique Lamaze Patrick Krätschmer James Jepson |
| author_sort | Ko-Fan Chen |
| collection | DOAJ |
| description | Sleep-like states in diverse organisms can be separated into distinct stages, each with a characteristic arousal threshold. However, the molecular pathways underlying different sleep stages remain unclear. The fruit fly, Drosophila melanogaster, exhibits consolidated sleep during both day and night, with night sleep associated with higher arousal thresholds compared to day sleep. Here we identify a role for the neuronal calcium sensor protein Neurocalcin (NCA) in promoting sleep during the night but not the day by suppressing nocturnal arousal and hyperactivity. We show that both circadian and light-sensing pathways define the temporal window in which NCA promotes sleep. Furthermore, we find that NCA promotes sleep by suppressing synaptic release from a dispersed wake-promoting neural network and demonstrate that the mushroom bodies, a sleep-regulatory center, are a module within this network. Our results advance the understanding of how sleep stages are genetically defined. |
| first_indexed | 2024-04-12T02:15:57Z |
| format | Article |
| id | doaj.art-a884e3c95fe54830b0643a28acf12bfa |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:15:57Z |
| publishDate | 2019-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a884e3c95fe54830b0643a28acf12bfa2022-12-22T03:52:16ZengeLife Sciences Publications LtdeLife2050-084X2019-03-01810.7554/eLife.38114Neurocalcin regulates nighttime sleep and arousal in DrosophilaKo-Fan Chen0https://orcid.org/0000-0002-7305-6254Simon Lowe1Angélique Lamaze2Patrick Krätschmer3James Jepson4https://orcid.org/0000-0002-3357-2801Department of Clinical and Experimental Epilepsy, UCL Institute of Neurology, London, United KingdomDepartment of Clinical and Experimental Epilepsy, UCL Institute of Neurology, London, United KingdomDepartment of Clinical and Experimental Epilepsy, UCL Institute of Neurology, London, United KingdomDepartment of Clinical and Experimental Epilepsy, UCL Institute of Neurology, London, United KingdomDepartment of Clinical and Experimental Epilepsy, UCL Institute of Neurology, London, United KingdomSleep-like states in diverse organisms can be separated into distinct stages, each with a characteristic arousal threshold. However, the molecular pathways underlying different sleep stages remain unclear. The fruit fly, Drosophila melanogaster, exhibits consolidated sleep during both day and night, with night sleep associated with higher arousal thresholds compared to day sleep. Here we identify a role for the neuronal calcium sensor protein Neurocalcin (NCA) in promoting sleep during the night but not the day by suppressing nocturnal arousal and hyperactivity. We show that both circadian and light-sensing pathways define the temporal window in which NCA promotes sleep. Furthermore, we find that NCA promotes sleep by suppressing synaptic release from a dispersed wake-promoting neural network and demonstrate that the mushroom bodies, a sleep-regulatory center, are a module within this network. Our results advance the understanding of how sleep stages are genetically defined.https://elifesciences.org/articles/38114sleeparousalneuronal calcium sensorcircadian clocklight |
| spellingShingle | Ko-Fan Chen Simon Lowe Angélique Lamaze Patrick Krätschmer James Jepson Neurocalcin regulates nighttime sleep and arousal in Drosophila eLife sleep arousal neuronal calcium sensor circadian clock light |
| title | Neurocalcin regulates nighttime sleep and arousal in Drosophila |
| title_full | Neurocalcin regulates nighttime sleep and arousal in Drosophila |
| title_fullStr | Neurocalcin regulates nighttime sleep and arousal in Drosophila |
| title_full_unstemmed | Neurocalcin regulates nighttime sleep and arousal in Drosophila |
| title_short | Neurocalcin regulates nighttime sleep and arousal in Drosophila |
| title_sort | neurocalcin regulates nighttime sleep and arousal in drosophila |
| topic | sleep arousal neuronal calcium sensor circadian clock light |
| url | https://elifesciences.org/articles/38114 |
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