Novel Fri1-like Viruses Infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12—Characterization, Comparative Genomic Analysis, and Host-Recognition Strategy.
Acinetobacter baumannii is a gram-negative, non-fermenting aerobic bacterium which is often associated with hospital-acquired infections and known for its ability to develop resistance to antibiotics, form biofilms, and survive for long periods in hospital environments. In this study, we present two...
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MDPI AG
2017-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/9/7/188 |
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| author | Anastasia V. Popova Daria G. Lavysh Evgeniy I. Klimuk Mikhail V. Edelstein Alexander G. Bogun Mikhail M. Shneider Artemiy E. Goncharov Sergey V. Leonov Konstantin V. Severinov |
| author_facet | Anastasia V. Popova Daria G. Lavysh Evgeniy I. Klimuk Mikhail V. Edelstein Alexander G. Bogun Mikhail M. Shneider Artemiy E. Goncharov Sergey V. Leonov Konstantin V. Severinov |
| author_sort | Anastasia V. Popova |
| collection | DOAJ |
| description | Acinetobacter baumannii is a gram-negative, non-fermenting aerobic bacterium which is often associated with hospital-acquired infections and known for its ability to develop resistance to antibiotics, form biofilms, and survive for long periods in hospital environments. In this study, we present two novel viruses, vB_AbaP_AS11 and vB_AbaP_AS12, specifically infecting and lysing distinct multidrug-resistant clinical A. baumannii strains with K19 and K27 capsular polysaccharide structures, respectively. Both phages demonstrate rapid adsorption, short latent periods, and high burst sizes in one-step growth experiments. The AS11 and AS12 linear double-stranded DNA genomes of 41,642 base pairs (bp) and 41,402 bp share 86.3% nucleotide sequence identity with the most variable regions falling in host receptor–recognition genes. These genes encode tail spikes possessing depolymerizing activities towards corresponding capsular polysaccharides which are the primary bacterial receptors. We described AS11 and AS12 genome organization and discuss the possible regulation of transcription. The overall genomic architecture and gene homology analyses showed that the phages are new representatives of the recently designated Fri1virus genus of the Autographivirinae subfamily within the Podoviridae family. |
| first_indexed | 2024-04-12T16:55:58Z |
| format | Article |
| id | doaj.art-a88a537042074061a1e0319b685126fd |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-12T16:55:58Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a88a537042074061a1e0319b685126fd2022-12-22T03:24:13ZengMDPI AGViruses1999-49152017-07-019718810.3390/v9070188v9070188Novel Fri1-like Viruses Infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12—Characterization, Comparative Genomic Analysis, and Host-Recognition Strategy.Anastasia V. Popova0Daria G. Lavysh1Evgeniy I. Klimuk2Mikhail V. Edelstein3Alexander G. Bogun4Mikhail M. Shneider5Artemiy E. Goncharov6Sergey V. Leonov7Konstantin V. Severinov8Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russian FederationInstitute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russian FederationInstitute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russian FederationInstitute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russian FederationState Researсh Center for Applied Microbiology and Biotechnology, Moscow region, Obolensk, Russian FederationShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian FederationNorth-Western State Medical University named after I.I. Mechnikov, Saint-Petersburg, Russian FederationMoscow Institute of Physics and Technology (State University), Moscow region, Dolgoprudny, Russian FederationInstitute of Molecular Genetics and Gene Biology, Russian Academy of Sciences, Moscow, Russian FederationAcinetobacter baumannii is a gram-negative, non-fermenting aerobic bacterium which is often associated with hospital-acquired infections and known for its ability to develop resistance to antibiotics, form biofilms, and survive for long periods in hospital environments. In this study, we present two novel viruses, vB_AbaP_AS11 and vB_AbaP_AS12, specifically infecting and lysing distinct multidrug-resistant clinical A. baumannii strains with K19 and K27 capsular polysaccharide structures, respectively. Both phages demonstrate rapid adsorption, short latent periods, and high burst sizes in one-step growth experiments. The AS11 and AS12 linear double-stranded DNA genomes of 41,642 base pairs (bp) and 41,402 bp share 86.3% nucleotide sequence identity with the most variable regions falling in host receptor–recognition genes. These genes encode tail spikes possessing depolymerizing activities towards corresponding capsular polysaccharides which are the primary bacterial receptors. We described AS11 and AS12 genome organization and discuss the possible regulation of transcription. The overall genomic architecture and gene homology analyses showed that the phages are new representatives of the recently designated Fri1virus genus of the Autographivirinae subfamily within the Podoviridae family.https://www.mdpi.com/1999-4915/9/7/188bacteriophageAcinetobacter baumanniiPodoviridaeFri1virusRNA polymerasetail spikecapsule types |
| spellingShingle | Anastasia V. Popova Daria G. Lavysh Evgeniy I. Klimuk Mikhail V. Edelstein Alexander G. Bogun Mikhail M. Shneider Artemiy E. Goncharov Sergey V. Leonov Konstantin V. Severinov Novel Fri1-like Viruses Infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12—Characterization, Comparative Genomic Analysis, and Host-Recognition Strategy. Viruses bacteriophage Acinetobacter baumannii Podoviridae Fri1virus RNA polymerase tail spike capsule types |
| title | Novel Fri1-like Viruses Infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12—Characterization, Comparative Genomic Analysis, and Host-Recognition Strategy. |
| title_full | Novel Fri1-like Viruses Infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12—Characterization, Comparative Genomic Analysis, and Host-Recognition Strategy. |
| title_fullStr | Novel Fri1-like Viruses Infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12—Characterization, Comparative Genomic Analysis, and Host-Recognition Strategy. |
| title_full_unstemmed | Novel Fri1-like Viruses Infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12—Characterization, Comparative Genomic Analysis, and Host-Recognition Strategy. |
| title_short | Novel Fri1-like Viruses Infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12—Characterization, Comparative Genomic Analysis, and Host-Recognition Strategy. |
| title_sort | novel fri1 like viruses infecting acinetobacter baumannii vb abap as11 and vb abap as12 characterization comparative genomic analysis and host recognition strategy |
| topic | bacteriophage Acinetobacter baumannii Podoviridae Fri1virus RNA polymerase tail spike capsule types |
| url | https://www.mdpi.com/1999-4915/9/7/188 |
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