Comparative studies on the production of statins using different microbial strains
In this study, we aimed to increase the yield of mevastatin and lovastatin in solid state fermentation (SSF) using three different fungi, Aspergillus terreus MTCC 279, Penicillium citrinum MTCC 1751, and Penicillium brevicompactum MTCC 549. Initially, we screened various substrates for maximizing th...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2019-09-01
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| Series: | BioTechnologia |
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| Online Access: | https://www.termedia.pl/Comparative-studies-on-the-production-of-statins-using-different-microbial-strains,85,37524,1,1.html |
| _version_ | 1819140273109729280 |
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| author | Mahin Syed |
| author_facet | Mahin Syed |
| author_sort | Mahin Syed |
| collection | DOAJ |
| description | In this study, we aimed to increase the yield of mevastatin and lovastatin in solid state fermentation (SSF) using three different fungi, Aspergillus terreus MTCC 279, Penicillium citrinum MTCC 1751, and Penicillium brevicompactum MTCC 549. Initially, we screened various substrates for maximizing the production of mevastatin and lovastatin, and barley powder was found to be the most suitable substrate for A. terreus. We applied response surface methodology (RSM) to determine the optimal parameters for initial moisture content, temperature, and inoculum size. The use of RSM resulted in maximizing the production of mevastatin to 288.13 mg/gram dry substrate (gds) and lovastatin to 329.51 mg/gds. For further studies, we used the high yielding strain of A. terreus with barley as a substrate. In the validation experiment, the maximum amount of mevastatin (297.98 mg/gds) and lovastatin (340.71 mg/gds) was obtained using A. terreus with barley as a substrate. This is the first report on the simultaneous production of mevastatin and lovastatin using a high yielding strain in SSF. |
| first_indexed | 2024-12-22T11:35:56Z |
| format | Article |
| id | doaj.art-a88aea68d57b4a348378efab76a2f768 |
| institution | Directory Open Access Journal |
| issn | 0860-7796 2353-9461 |
| language | English |
| last_indexed | 2024-12-22T11:35:56Z |
| publishDate | 2019-09-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | BioTechnologia |
| spelling | doaj.art-a88aea68d57b4a348378efab76a2f7682022-12-21T18:27:27ZengTermedia Publishing HouseBioTechnologia0860-77962353-94612019-09-01100325126210.5114/bta.2019.8758437524Comparative studies on the production of statins using different microbial strainsMahin SyedIn this study, we aimed to increase the yield of mevastatin and lovastatin in solid state fermentation (SSF) using three different fungi, Aspergillus terreus MTCC 279, Penicillium citrinum MTCC 1751, and Penicillium brevicompactum MTCC 549. Initially, we screened various substrates for maximizing the production of mevastatin and lovastatin, and barley powder was found to be the most suitable substrate for A. terreus. We applied response surface methodology (RSM) to determine the optimal parameters for initial moisture content, temperature, and inoculum size. The use of RSM resulted in maximizing the production of mevastatin to 288.13 mg/gram dry substrate (gds) and lovastatin to 329.51 mg/gds. For further studies, we used the high yielding strain of A. terreus with barley as a substrate. In the validation experiment, the maximum amount of mevastatin (297.98 mg/gds) and lovastatin (340.71 mg/gds) was obtained using A. terreus with barley as a substrate. This is the first report on the simultaneous production of mevastatin and lovastatin using a high yielding strain in SSF.https://www.termedia.pl/Comparative-studies-on-the-production-of-statins-using-different-microbial-strains,85,37524,1,1.htmlsolid state fermentation mevastatin aspergillus terreus penicillium citrinum penicillium brevicompactum response surface methodology |
| spellingShingle | Mahin Syed Comparative studies on the production of statins using different microbial strains BioTechnologia solid state fermentation mevastatin aspergillus terreus penicillium citrinum penicillium brevicompactum response surface methodology |
| title | Comparative studies on the production of statins using different microbial strains |
| title_full | Comparative studies on the production of statins using different microbial strains |
| title_fullStr | Comparative studies on the production of statins using different microbial strains |
| title_full_unstemmed | Comparative studies on the production of statins using different microbial strains |
| title_short | Comparative studies on the production of statins using different microbial strains |
| title_sort | comparative studies on the production of statins using different microbial strains |
| topic | solid state fermentation mevastatin aspergillus terreus penicillium citrinum penicillium brevicompactum response surface methodology |
| url | https://www.termedia.pl/Comparative-studies-on-the-production-of-statins-using-different-microbial-strains,85,37524,1,1.html |
| work_keys_str_mv | AT mahinsyed comparativestudiesontheproductionofstatinsusingdifferentmicrobialstrains |