Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells
The excellent photothermal properties of gold nanorods (Au-NRs) make them one of the most researched plasmonic photothermal nanomaterials. However, their biological applications have been hampered greatly due to surfactant-induced cytotoxicity. We herein report a simple synthesis of highly biocompat...
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MDPI AG
2021-11-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/14/11/1137 |
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| author | Adewale Oladipo Thabang Calvin Lebepe Sundararajan Parani Rodney Maluleke Vuyelwa Ncapayi Grace It Mwad Mbaz Sandile Phinda Songca Tetsuya Kodama Oluwatobi Samuel Oluwafemi |
| author_facet | Adewale Oladipo Thabang Calvin Lebepe Sundararajan Parani Rodney Maluleke Vuyelwa Ncapayi Grace It Mwad Mbaz Sandile Phinda Songca Tetsuya Kodama Oluwatobi Samuel Oluwafemi |
| author_sort | Adewale Oladipo |
| collection | DOAJ |
| description | The excellent photothermal properties of gold nanorods (Au-NRs) make them one of the most researched plasmonic photothermal nanomaterials. However, their biological applications have been hampered greatly due to surfactant-induced cytotoxicity. We herein report a simple synthesis of highly biocompatible gelatin stabilized Au-NRs (gelatin@Au-NRs) to address this issue. The optical and structural properties of the as-synthesized gelatin@Au-NRs were investigated by Zetasizer, Ultraviolet-Visible-Near Infrared (UV-Vis-NIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and Fourier transform infrared spectroscopy (FTIR). The as-synthesized gelatin@Au-NRs were highly crystalline and rod-like in shape with an average length and diameter of 66.2 ± 2.3 nm and 10 ± 1.6 nm, respectively. The as-synthesized gelatin@Au-NRs showed high stability in common biological media (phosphate buffer saline and Dulbecco’s Modified Eagle’s Medium) compared to CTAB capped Au-NRs. Similarly, the gelatin@Au-NRs showed an improved heat production and outstanding cell viability against two different cancer cell lines; KM-Luc/GFP (mouse fibroblast histiocytoma cell line) and FM3A-Luc (breast carcinoma cell line) compared to CTAB capped Au-NRs and PEG@Au-NRs. An in vitro photothermal therapy study against KM-Luc/GFP showed that gelatin@Au-NRs effectively destroys the cancer cells. |
| first_indexed | 2024-03-10T05:10:14Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T05:10:14Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-a88af0ca6f8b4c39bfc0d44199de71df2023-11-23T00:55:47ZengMDPI AGPharmaceuticals1424-82472021-11-011411113710.3390/ph14111137Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma CellsAdewale Oladipo0Thabang Calvin Lebepe1Sundararajan Parani2Rodney Maluleke3Vuyelwa Ncapayi4Grace It Mwad Mbaz5Sandile Phinda Songca6Tetsuya Kodama7Oluwatobi Samuel Oluwafemi8Department of Chemical Sciences, Doornfontein Campus, University of Johannesburg, Johannesburg 2028, South AfricaDepartment of Chemical Sciences, Doornfontein Campus, University of Johannesburg, Johannesburg 2028, South AfricaDepartment of Chemical Sciences, Doornfontein Campus, University of Johannesburg, Johannesburg 2028, South AfricaDepartment of Chemical Sciences, Doornfontein Campus, University of Johannesburg, Johannesburg 2028, South AfricaDepartment of Chemical Sciences, Doornfontein Campus, University of Johannesburg, Johannesburg 2028, South AfricaDepartment of Chemical Sciences, Doornfontein Campus, University of Johannesburg, Johannesburg 2028, South AfricaDepartment of Chemistry, University of KwaZulu-Natal, Private Bag X 54001, Durban 4000, South AfricaGraduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo, Aoba, Sendai 980-8575, JapanDepartment of Chemical Sciences, Doornfontein Campus, University of Johannesburg, Johannesburg 2028, South AfricaThe excellent photothermal properties of gold nanorods (Au-NRs) make them one of the most researched plasmonic photothermal nanomaterials. However, their biological applications have been hampered greatly due to surfactant-induced cytotoxicity. We herein report a simple synthesis of highly biocompatible gelatin stabilized Au-NRs (gelatin@Au-NRs) to address this issue. The optical and structural properties of the as-synthesized gelatin@Au-NRs were investigated by Zetasizer, Ultraviolet-Visible-Near Infrared (UV-Vis-NIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and Fourier transform infrared spectroscopy (FTIR). The as-synthesized gelatin@Au-NRs were highly crystalline and rod-like in shape with an average length and diameter of 66.2 ± 2.3 nm and 10 ± 1.6 nm, respectively. The as-synthesized gelatin@Au-NRs showed high stability in common biological media (phosphate buffer saline and Dulbecco’s Modified Eagle’s Medium) compared to CTAB capped Au-NRs. Similarly, the gelatin@Au-NRs showed an improved heat production and outstanding cell viability against two different cancer cell lines; KM-Luc/GFP (mouse fibroblast histiocytoma cell line) and FM3A-Luc (breast carcinoma cell line) compared to CTAB capped Au-NRs and PEG@Au-NRs. An in vitro photothermal therapy study against KM-Luc/GFP showed that gelatin@Au-NRs effectively destroys the cancer cells.https://www.mdpi.com/1424-8247/14/11/1137gold nanorodsgelatinnear-infraredstabilitycytotoxicityphotothermal therapy |
| spellingShingle | Adewale Oladipo Thabang Calvin Lebepe Sundararajan Parani Rodney Maluleke Vuyelwa Ncapayi Grace It Mwad Mbaz Sandile Phinda Songca Tetsuya Kodama Oluwatobi Samuel Oluwafemi Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells Pharmaceuticals gold nanorods gelatin near-infrared stability cytotoxicity photothermal therapy |
| title | Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells |
| title_full | Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells |
| title_fullStr | Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells |
| title_full_unstemmed | Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells |
| title_short | Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells |
| title_sort | synthesis of nir ii absorbing gelatin stabilized gold nanorods and its photothermal therapy application against fibroblast histiocytoma cells |
| topic | gold nanorods gelatin near-infrared stability cytotoxicity photothermal therapy |
| url | https://www.mdpi.com/1424-8247/14/11/1137 |
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