The MADS-box genes SOC1 and AGL24 antagonize XAL2 functions in Arabidopsis thaliana root development
MADS-domain transcription factors play pivotal roles in numerous developmental processes in Arabidopsis thaliana. While their involvement in flowering transition and floral development has been extensively examined, their functions in root development remain relatively unexplored. Here, we explored...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-03-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1331269/full |
| _version_ | 1797253722649133056 |
|---|---|
| author | Claudio A. Castañón-Suárez Claudio A. Castañón-Suárez Maite Arrizubieta Natalia Castelán-Muñoz Natalia Castelán-Muñoz Diana Belén Sánchez-Rodríguez Carolina Caballero-Cordero Estephania Zluhan-Martínez Sandra C. Patiño-Olvera J.Arturo Arciniega-González Berenice García-Ponce María de la Paz Sánchez Elena R. Álvarez-Buylla Elena R. Álvarez-Buylla Adriana Garay-Arroyo |
| author_facet | Claudio A. Castañón-Suárez Claudio A. Castañón-Suárez Maite Arrizubieta Natalia Castelán-Muñoz Natalia Castelán-Muñoz Diana Belén Sánchez-Rodríguez Carolina Caballero-Cordero Estephania Zluhan-Martínez Sandra C. Patiño-Olvera J.Arturo Arciniega-González Berenice García-Ponce María de la Paz Sánchez Elena R. Álvarez-Buylla Elena R. Álvarez-Buylla Adriana Garay-Arroyo |
| author_sort | Claudio A. Castañón-Suárez |
| collection | DOAJ |
| description | MADS-domain transcription factors play pivotal roles in numerous developmental processes in Arabidopsis thaliana. While their involvement in flowering transition and floral development has been extensively examined, their functions in root development remain relatively unexplored. Here, we explored the function and genetic interaction of three MADS-box genes (XAL2, SOC1 and AGL24) in primary root development. By analyzing loss-of-function and overexpression lines, we found that SOC1 and AGL24, both critical components in flowering transition, redundantly act as repressors of primary root growth as the loss of function of either SOC1 or AGL24 partially recovers the primary root growth, meristem cell number, cell production rate, and the length of fully elongated cells of the short-root mutant xal2-2. Furthermore, we observed that the simultaneous overexpression of AGL24 and SOC1 leads to short-root phenotypes, affecting meristem cell number and fully elongated cell size, whereas SOC1 overexpression is sufficient to affect columella stem cell differentiation. Additionally, qPCR analyses revealed that these genes exhibit distinct modes of transcriptional regulation in roots compared to what has been previously reported for aerial tissues. We identified 100 differentially expressed genes in xal2-2 roots by RNA-seq. Moreover, our findings revealed that the expression of certain genes involved in cell differentiation, as well as stress responses, which are either upregulated or downregulated in the xal2-2 mutant, reverted to WT levels in the absence of SOC1 or AGL24. |
| first_indexed | 2024-04-24T21:38:35Z |
| format | Article |
| id | doaj.art-a88ff01a2c964380ba1d0ee3b3bc04e9 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-24T21:38:35Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a88ff01a2c964380ba1d0ee3b3bc04e92024-03-21T15:28:35ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-03-011510.3389/fpls.2024.13312691331269The MADS-box genes SOC1 and AGL24 antagonize XAL2 functions in Arabidopsis thaliana root developmentClaudio A. Castañón-Suárez0Claudio A. Castañón-Suárez1Maite Arrizubieta2Natalia Castelán-Muñoz3Natalia Castelán-Muñoz4Diana Belén Sánchez-Rodríguez5Carolina Caballero-Cordero6Estephania Zluhan-Martínez7Sandra C. Patiño-Olvera8J.Arturo Arciniega-González9Berenice García-Ponce10María de la Paz Sánchez11Elena R. Álvarez-Buylla12Elena R. Álvarez-Buylla13Adriana Garay-Arroyo14Laboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, MexicoPosgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Mexico City, MexicoLaboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, MexicoLaboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, MexicoPostgrado en Recursos Genéticos y Productividad-Fisiología Vegetal, Colegio de Postgraduados, Texcoco, Estado de México, MexicoLaboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, MexicoCentro de Ciencias de la Complejidad (C3), Universidad Nacional Autónoma de México, Ciudad de México, MexicoLaboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, MexicoLaboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, MexicoCentro de Ciencias de la Complejidad (C3), Universidad Nacional Autónoma de México, Ciudad de México, MexicoLaboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, MexicoLaboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, MexicoLaboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, MexicoCentro de Ciencias de la Complejidad (C3), Universidad Nacional Autónoma de México, Ciudad de México, MexicoLaboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México, MexicoMADS-domain transcription factors play pivotal roles in numerous developmental processes in Arabidopsis thaliana. While their involvement in flowering transition and floral development has been extensively examined, their functions in root development remain relatively unexplored. Here, we explored the function and genetic interaction of three MADS-box genes (XAL2, SOC1 and AGL24) in primary root development. By analyzing loss-of-function and overexpression lines, we found that SOC1 and AGL24, both critical components in flowering transition, redundantly act as repressors of primary root growth as the loss of function of either SOC1 or AGL24 partially recovers the primary root growth, meristem cell number, cell production rate, and the length of fully elongated cells of the short-root mutant xal2-2. Furthermore, we observed that the simultaneous overexpression of AGL24 and SOC1 leads to short-root phenotypes, affecting meristem cell number and fully elongated cell size, whereas SOC1 overexpression is sufficient to affect columella stem cell differentiation. Additionally, qPCR analyses revealed that these genes exhibit distinct modes of transcriptional regulation in roots compared to what has been previously reported for aerial tissues. We identified 100 differentially expressed genes in xal2-2 roots by RNA-seq. Moreover, our findings revealed that the expression of certain genes involved in cell differentiation, as well as stress responses, which are either upregulated or downregulated in the xal2-2 mutant, reverted to WT levels in the absence of SOC1 or AGL24.https://www.frontiersin.org/articles/10.3389/fpls.2024.1331269/fullMADS-domain proteinsroot growthprimary root developmentstem cell nichecolumella stem cell differentiationcell wall |
| spellingShingle | Claudio A. Castañón-Suárez Claudio A. Castañón-Suárez Maite Arrizubieta Natalia Castelán-Muñoz Natalia Castelán-Muñoz Diana Belén Sánchez-Rodríguez Carolina Caballero-Cordero Estephania Zluhan-Martínez Sandra C. Patiño-Olvera J.Arturo Arciniega-González Berenice García-Ponce María de la Paz Sánchez Elena R. Álvarez-Buylla Elena R. Álvarez-Buylla Adriana Garay-Arroyo The MADS-box genes SOC1 and AGL24 antagonize XAL2 functions in Arabidopsis thaliana root development Frontiers in Plant Science MADS-domain proteins root growth primary root development stem cell niche columella stem cell differentiation cell wall |
| title | The MADS-box genes SOC1 and AGL24 antagonize XAL2 functions in Arabidopsis thaliana root development |
| title_full | The MADS-box genes SOC1 and AGL24 antagonize XAL2 functions in Arabidopsis thaliana root development |
| title_fullStr | The MADS-box genes SOC1 and AGL24 antagonize XAL2 functions in Arabidopsis thaliana root development |
| title_full_unstemmed | The MADS-box genes SOC1 and AGL24 antagonize XAL2 functions in Arabidopsis thaliana root development |
| title_short | The MADS-box genes SOC1 and AGL24 antagonize XAL2 functions in Arabidopsis thaliana root development |
| title_sort | mads box genes soc1 and agl24 antagonize xal2 functions in arabidopsis thaliana root development |
| topic | MADS-domain proteins root growth primary root development stem cell niche columella stem cell differentiation cell wall |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1331269/full |
| work_keys_str_mv | AT claudioacastanonsuarez themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT claudioacastanonsuarez themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT maitearrizubieta themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT nataliacastelanmunoz themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT nataliacastelanmunoz themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT dianabelensanchezrodriguez themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT carolinacaballerocordero themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT estephaniazluhanmartinez themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT sandracpatinoolvera themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT jarturoarciniegagonzalez themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT berenicegarciaponce themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT mariadelapazsanchez themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT elenaralvarezbuylla themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT elenaralvarezbuylla themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT adrianagarayarroyo themadsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT claudioacastanonsuarez madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT claudioacastanonsuarez madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT maitearrizubieta madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT nataliacastelanmunoz madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT nataliacastelanmunoz madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT dianabelensanchezrodriguez madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT carolinacaballerocordero madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT estephaniazluhanmartinez madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT sandracpatinoolvera madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT jarturoarciniegagonzalez madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT berenicegarciaponce madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT mariadelapazsanchez madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT elenaralvarezbuylla madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT elenaralvarezbuylla madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment AT adrianagarayarroyo madsboxgenessoc1andagl24antagonizexal2functionsinarabidopsisthalianarootdevelopment |