An Integrated Analysis of microRNAs and the Transcriptome Reveals the Molecular Mechanisms Underlying the Regulation of Leaf Development in Xinyang Maojian Green Tea (<i>Camellia sinensis</i>)
Xinyang Maojian (XYMJ) tea is one of the world’s most popular green teas; the development of new sprouts directly affects the yield and quality of tea products, especially for XYMJ, which has hairy tips. Here, we used transcriptome and small RNA sequencing to identify mRNAs and miRNAs, respectively,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/21/3665 |
| _version_ | 1797631435951046656 |
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| author | Xianyou Wang Ruijin Zhou Shanshan Zhao Shengyang Niu |
| author_facet | Xianyou Wang Ruijin Zhou Shanshan Zhao Shengyang Niu |
| author_sort | Xianyou Wang |
| collection | DOAJ |
| description | Xinyang Maojian (XYMJ) tea is one of the world’s most popular green teas; the development of new sprouts directly affects the yield and quality of tea products, especially for XYMJ, which has hairy tips. Here, we used transcriptome and small RNA sequencing to identify mRNAs and miRNAs, respectively, involved in regulating leaf development in different plant tissues (bud, leaf, and stem). We identified a total of 381 conserved miRNAs. Given that no genomic data for XYMJ green tea are available, we compared the sequencing data for XYMJ green tea with genomic data from a closely related species (Tieguanyin) and the <i>Camellia sinensis</i> var. <i>sinensis</i> database; we identified a total of 506 and 485 novel miRNAs, respectively. We also identified 11 sequence-identical novel miRNAs in the tissues of XYMJ tea plants. Correlation analyses revealed 97 miRNA–mRNA pairs involved in leaf growth and development; the csn-miR319-2/csnTCP2 and miR159–csnMYB modules were found to be involved in leaf development in XYMJ green tea. Quantitative real-time PCR was used to validate the expression levels of the miRNAs and mRNAs. The miRNAs and target genes identified in this study might shed new light on the molecular mechanisms underlying the regulation of leaf development in tea plants. |
| first_indexed | 2024-03-11T11:23:36Z |
| format | Article |
| id | doaj.art-a891180481684feaa492efbfffcb4a3c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T11:23:36Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-a891180481684feaa492efbfffcb4a3c2023-11-10T15:10:07ZengMDPI AGPlants2223-77472023-10-011221366510.3390/plants12213665An Integrated Analysis of microRNAs and the Transcriptome Reveals the Molecular Mechanisms Underlying the Regulation of Leaf Development in Xinyang Maojian Green Tea (<i>Camellia sinensis</i>)Xianyou Wang0Ruijin Zhou1Shanshan Zhao2Shengyang Niu3School of Horticulture Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, ChinaSchool of Horticulture Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, ChinaSchool of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, ChinaSchool of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, ChinaXinyang Maojian (XYMJ) tea is one of the world’s most popular green teas; the development of new sprouts directly affects the yield and quality of tea products, especially for XYMJ, which has hairy tips. Here, we used transcriptome and small RNA sequencing to identify mRNAs and miRNAs, respectively, involved in regulating leaf development in different plant tissues (bud, leaf, and stem). We identified a total of 381 conserved miRNAs. Given that no genomic data for XYMJ green tea are available, we compared the sequencing data for XYMJ green tea with genomic data from a closely related species (Tieguanyin) and the <i>Camellia sinensis</i> var. <i>sinensis</i> database; we identified a total of 506 and 485 novel miRNAs, respectively. We also identified 11 sequence-identical novel miRNAs in the tissues of XYMJ tea plants. Correlation analyses revealed 97 miRNA–mRNA pairs involved in leaf growth and development; the csn-miR319-2/csnTCP2 and miR159–csnMYB modules were found to be involved in leaf development in XYMJ green tea. Quantitative real-time PCR was used to validate the expression levels of the miRNAs and mRNAs. The miRNAs and target genes identified in this study might shed new light on the molecular mechanisms underlying the regulation of leaf development in tea plants.https://www.mdpi.com/2223-7747/12/21/3665tea plantmiRNAXinyang Maojianleaf developmentregulatory network |
| spellingShingle | Xianyou Wang Ruijin Zhou Shanshan Zhao Shengyang Niu An Integrated Analysis of microRNAs and the Transcriptome Reveals the Molecular Mechanisms Underlying the Regulation of Leaf Development in Xinyang Maojian Green Tea (<i>Camellia sinensis</i>) Plants tea plant miRNA Xinyang Maojian leaf development regulatory network |
| title | An Integrated Analysis of microRNAs and the Transcriptome Reveals the Molecular Mechanisms Underlying the Regulation of Leaf Development in Xinyang Maojian Green Tea (<i>Camellia sinensis</i>) |
| title_full | An Integrated Analysis of microRNAs and the Transcriptome Reveals the Molecular Mechanisms Underlying the Regulation of Leaf Development in Xinyang Maojian Green Tea (<i>Camellia sinensis</i>) |
| title_fullStr | An Integrated Analysis of microRNAs and the Transcriptome Reveals the Molecular Mechanisms Underlying the Regulation of Leaf Development in Xinyang Maojian Green Tea (<i>Camellia sinensis</i>) |
| title_full_unstemmed | An Integrated Analysis of microRNAs and the Transcriptome Reveals the Molecular Mechanisms Underlying the Regulation of Leaf Development in Xinyang Maojian Green Tea (<i>Camellia sinensis</i>) |
| title_short | An Integrated Analysis of microRNAs and the Transcriptome Reveals the Molecular Mechanisms Underlying the Regulation of Leaf Development in Xinyang Maojian Green Tea (<i>Camellia sinensis</i>) |
| title_sort | integrated analysis of micrornas and the transcriptome reveals the molecular mechanisms underlying the regulation of leaf development in xinyang maojian green tea i camellia sinensis i |
| topic | tea plant miRNA Xinyang Maojian leaf development regulatory network |
| url | https://www.mdpi.com/2223-7747/12/21/3665 |
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