NTPDase and acetylcholinesterase activities in silver catfish, Rhamdia quelen (Quoy & Gaimard, 1824) (Heptapteridae) exposed to interaction of oxygen and ammonia levels
The effects of various levels of oxygen saturation and ammonia concentration on NTPDase (ecto-nucleoside triphosphate diphosphohydrolase, E.C. 3.6.1.5) and acetylcholinesterase (AChE, E.C. 3.1.1.7) activities in whole brain of teleost fish (Rhamdia quelen) were investigated. The fish were exposed to...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Ictiologia
|
| Series: | Neotropical Ichthyology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400012&lng=en&tlng=en |
| _version_ | 1828387051613978624 |
|---|---|
| author | Rosilene Rodrigues Kaizer Vania Lúcia Loro Maria Rosa Chitolina Schetinger Vera Maria Morsch Luciane Almeri Tabaldi Cíntia Saydelles da Rosa Luciano de Oliveira Garcia Alexssandro G. Becker Bernardo Baldisserotto |
| author_facet | Rosilene Rodrigues Kaizer Vania Lúcia Loro Maria Rosa Chitolina Schetinger Vera Maria Morsch Luciane Almeri Tabaldi Cíntia Saydelles da Rosa Luciano de Oliveira Garcia Alexssandro G. Becker Bernardo Baldisserotto |
| author_sort | Rosilene Rodrigues Kaizer |
| collection | DOAJ |
| description | The effects of various levels of oxygen saturation and ammonia concentration on NTPDase (ecto-nucleoside triphosphate diphosphohydrolase, E.C. 3.6.1.5) and acetylcholinesterase (AChE, E.C. 3.1.1.7) activities in whole brain of teleost fish (Rhamdia quelen) were investigated. The fish were exposed to one of two different dissolved oxygen levels, including high oxygen (6.5 mg.L-1) or low oxygen (3.5 mg.L-1), and one of two different ammonia levels, including high ammonia (0.1 mg.L-1) or low ammonia (0.03 mg.L-1) levels. The four experimental groups included the following (A) control, or high dissolved oxygen plus low NH3; (B) low dissolved oxygen plus low NH3; (C) high dissolved oxygen plus high NH3; (D) low dissolved oxygen plus high NH3. We found that enzyme activities were altered after 24 h exposure in groups C and D. ATP and ADP hydrolysis in whole brain of fish was enhanced in group D after 24 h exposure by 100% and 119%, respectively, compared to the control group. After 24 h exposure, AChE activity presented an increase of 34% and 39% in groups C and D, respectively, when compared to the control group. These results are consistent with the hypothesis that low oxygen levels increase ammonia toxicity. Moreover, the hypoxic events may increase blood flow by hypoxia increasing NTPDase activity, thus producing adenosine, a potent vasodilator. |
| first_indexed | 2024-12-10T05:49:06Z |
| format | Article |
| id | doaj.art-a8925cdd6f4a4a759199929de87cd379 |
| institution | Directory Open Access Journal |
| issn | 1982-0224 |
| language | English |
| last_indexed | 2024-12-10T05:49:06Z |
| publisher | Sociedade Brasileira de Ictiologia |
| record_format | Article |
| series | Neotropical Ichthyology |
| spelling | doaj.art-a8925cdd6f4a4a759199929de87cd3792022-12-22T02:00:06ZengSociedade Brasileira de IctiologiaNeotropical Ichthyology1982-02247463564010.1590/S1679-62252009000400012S1679-62252009000400012NTPDase and acetylcholinesterase activities in silver catfish, Rhamdia quelen (Quoy & Gaimard, 1824) (Heptapteridae) exposed to interaction of oxygen and ammonia levelsRosilene Rodrigues Kaizer0Vania Lúcia Loro1Maria Rosa Chitolina Schetinger2Vera Maria Morsch3Luciane Almeri Tabaldi4Cíntia Saydelles da Rosa5Luciano de Oliveira Garcia6Alexssandro G. Becker7Bernardo Baldisserotto8Universidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaThe effects of various levels of oxygen saturation and ammonia concentration on NTPDase (ecto-nucleoside triphosphate diphosphohydrolase, E.C. 3.6.1.5) and acetylcholinesterase (AChE, E.C. 3.1.1.7) activities in whole brain of teleost fish (Rhamdia quelen) were investigated. The fish were exposed to one of two different dissolved oxygen levels, including high oxygen (6.5 mg.L-1) or low oxygen (3.5 mg.L-1), and one of two different ammonia levels, including high ammonia (0.1 mg.L-1) or low ammonia (0.03 mg.L-1) levels. The four experimental groups included the following (A) control, or high dissolved oxygen plus low NH3; (B) low dissolved oxygen plus low NH3; (C) high dissolved oxygen plus high NH3; (D) low dissolved oxygen plus high NH3. We found that enzyme activities were altered after 24 h exposure in groups C and D. ATP and ADP hydrolysis in whole brain of fish was enhanced in group D after 24 h exposure by 100% and 119%, respectively, compared to the control group. After 24 h exposure, AChE activity presented an increase of 34% and 39% in groups C and D, respectively, when compared to the control group. These results are consistent with the hypothesis that low oxygen levels increase ammonia toxicity. Moreover, the hypoxic events may increase blood flow by hypoxia increasing NTPDase activity, thus producing adenosine, a potent vasodilator.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400012&lng=en&tlng=enFishATPADPAChE |
| spellingShingle | Rosilene Rodrigues Kaizer Vania Lúcia Loro Maria Rosa Chitolina Schetinger Vera Maria Morsch Luciane Almeri Tabaldi Cíntia Saydelles da Rosa Luciano de Oliveira Garcia Alexssandro G. Becker Bernardo Baldisserotto NTPDase and acetylcholinesterase activities in silver catfish, Rhamdia quelen (Quoy & Gaimard, 1824) (Heptapteridae) exposed to interaction of oxygen and ammonia levels Neotropical Ichthyology Fish ATP ADP AChE |
| title | NTPDase and acetylcholinesterase activities in silver catfish, Rhamdia quelen (Quoy & Gaimard, 1824) (Heptapteridae) exposed to interaction of oxygen and ammonia levels |
| title_full | NTPDase and acetylcholinesterase activities in silver catfish, Rhamdia quelen (Quoy & Gaimard, 1824) (Heptapteridae) exposed to interaction of oxygen and ammonia levels |
| title_fullStr | NTPDase and acetylcholinesterase activities in silver catfish, Rhamdia quelen (Quoy & Gaimard, 1824) (Heptapteridae) exposed to interaction of oxygen and ammonia levels |
| title_full_unstemmed | NTPDase and acetylcholinesterase activities in silver catfish, Rhamdia quelen (Quoy & Gaimard, 1824) (Heptapteridae) exposed to interaction of oxygen and ammonia levels |
| title_short | NTPDase and acetylcholinesterase activities in silver catfish, Rhamdia quelen (Quoy & Gaimard, 1824) (Heptapteridae) exposed to interaction of oxygen and ammonia levels |
| title_sort | ntpdase and acetylcholinesterase activities in silver catfish rhamdia quelen quoy gaimard 1824 heptapteridae exposed to interaction of oxygen and ammonia levels |
| topic | Fish ATP ADP AChE |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400012&lng=en&tlng=en |
| work_keys_str_mv | AT rosilenerodrigueskaizer ntpdaseandacetylcholinesteraseactivitiesinsilvercatfishrhamdiaquelenquoygaimard1824heptapteridaeexposedtointeractionofoxygenandammonialevels AT vanialucialoro ntpdaseandacetylcholinesteraseactivitiesinsilvercatfishrhamdiaquelenquoygaimard1824heptapteridaeexposedtointeractionofoxygenandammonialevels AT mariarosachitolinaschetinger ntpdaseandacetylcholinesteraseactivitiesinsilvercatfishrhamdiaquelenquoygaimard1824heptapteridaeexposedtointeractionofoxygenandammonialevels AT veramariamorsch ntpdaseandacetylcholinesteraseactivitiesinsilvercatfishrhamdiaquelenquoygaimard1824heptapteridaeexposedtointeractionofoxygenandammonialevels AT lucianealmeritabaldi ntpdaseandacetylcholinesteraseactivitiesinsilvercatfishrhamdiaquelenquoygaimard1824heptapteridaeexposedtointeractionofoxygenandammonialevels AT cintiasaydellesdarosa ntpdaseandacetylcholinesteraseactivitiesinsilvercatfishrhamdiaquelenquoygaimard1824heptapteridaeexposedtointeractionofoxygenandammonialevels AT lucianodeoliveiragarcia ntpdaseandacetylcholinesteraseactivitiesinsilvercatfishrhamdiaquelenquoygaimard1824heptapteridaeexposedtointeractionofoxygenandammonialevels AT alexssandrogbecker ntpdaseandacetylcholinesteraseactivitiesinsilvercatfishrhamdiaquelenquoygaimard1824heptapteridaeexposedtointeractionofoxygenandammonialevels AT bernardobaldisserotto ntpdaseandacetylcholinesteraseactivitiesinsilvercatfishrhamdiaquelenquoygaimard1824heptapteridaeexposedtointeractionofoxygenandammonialevels |