RNA Interference as a New Tool in Therapeutics
The basic principle of RNA interference, a possible new therapeutic tool, involves destruction of messenger RNA upon interaction with homologous double-stranded RNA present in the cell cytoplasm. Studies have shown that both viral and non-viral small interfering RNA delivery methods and delivery of...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2009-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/62455 |
| _version_ | 1797428681445998592 |
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| author | Karmela Barišić Mirela Matokanović |
| author_facet | Karmela Barišić Mirela Matokanović |
| author_sort | Karmela Barišić |
| collection | DOAJ |
| description | The basic principle of RNA interference, a possible new therapeutic tool, involves destruction of messenger RNA upon interaction with homologous double-stranded RNA present in the cell cytoplasm. Studies have shown that both viral and non-viral small interfering RNA delivery methods and delivery of chemically synthesized small interfering RNAs to the cell can provide selective gene suppression through this mechanism, both in vitro and in vivo. Before becoming a functional therapeutic tool, there are a number of problems concerning RNA interference that should be solved. Major problems involve off-target effects, insertional mutagenesis and malignant transformation, as well as problems of delivery methods and reduction of toxicity. |
| first_indexed | 2024-03-09T09:02:23Z |
| format | Article |
| id | doaj.art-a8949ddf413e4e9b8307d2c0fdaaa11e |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T09:02:23Z |
| publishDate | 2009-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-a8949ddf413e4e9b8307d2c0fdaaa11e2023-12-02T11:21:40ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062009-01-01473229235RNA Interference as a New Tool in TherapeuticsKarmela Barišić0Mirela Matokanović1Department of Medical Biochemistry and Haematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, HR-10000 Zagreb, CroatiaDepartment of Medical Biochemistry and Haematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, HR-10000 Zagreb, CroatiaThe basic principle of RNA interference, a possible new therapeutic tool, involves destruction of messenger RNA upon interaction with homologous double-stranded RNA present in the cell cytoplasm. Studies have shown that both viral and non-viral small interfering RNA delivery methods and delivery of chemically synthesized small interfering RNAs to the cell can provide selective gene suppression through this mechanism, both in vitro and in vivo. Before becoming a functional therapeutic tool, there are a number of problems concerning RNA interference that should be solved. Major problems involve off-target effects, insertional mutagenesis and malignant transformation, as well as problems of delivery methods and reduction of toxicity.http://hrcak.srce.hr/file/62455RNA interferencesmall interfering RNAoff-target effectstherapeutics |
| spellingShingle | Karmela Barišić Mirela Matokanović RNA Interference as a New Tool in Therapeutics Food Technology and Biotechnology RNA interference small interfering RNA off-target effects therapeutics |
| title | RNA Interference as a New Tool in Therapeutics |
| title_full | RNA Interference as a New Tool in Therapeutics |
| title_fullStr | RNA Interference as a New Tool in Therapeutics |
| title_full_unstemmed | RNA Interference as a New Tool in Therapeutics |
| title_short | RNA Interference as a New Tool in Therapeutics |
| title_sort | rna interference as a new tool in therapeutics |
| topic | RNA interference small interfering RNA off-target effects therapeutics |
| url | http://hrcak.srce.hr/file/62455 |
| work_keys_str_mv | AT karmelabarisic rnainterferenceasanewtoolintherapeutics AT mirelamatokanovic rnainterferenceasanewtoolintherapeutics |