Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms
Aims: We sought to characterize the antibiotic susceptibility of strains of Stenotrophomonas maltophilia isolated from clinical samples, and the role of Stenotrophomonas maltophilia biofilm in antibiotic resistance. Methods: Fifty-one clinical Stenotrophomonas maltophilia isolates were obtained from...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-07-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867016300721 |
| _version_ | 1819024271260778496 |
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| author | Erlin Sun Gehong Liang Lining Wang Wenjie Wei Mingde Lei Shiduo Song Ruifa Han Yubao Wang Wei Qi |
| author_facet | Erlin Sun Gehong Liang Lining Wang Wenjie Wei Mingde Lei Shiduo Song Ruifa Han Yubao Wang Wei Qi |
| author_sort | Erlin Sun |
| collection | DOAJ |
| description | Aims: We sought to characterize the antibiotic susceptibility of strains of Stenotrophomonas maltophilia isolated from clinical samples, and the role of Stenotrophomonas maltophilia biofilm in antibiotic resistance. Methods: Fifty-one clinical Stenotrophomonas maltophilia isolates were obtained from patients with nosocomial infection in the surgical wards and ICUs of six general hospitals in Tianjin, China. In vitro models of Stenotrophomonas maltophilia biofilms were established and confirmed by scanning electron microscopy and fluorescence microscopy with silver staining. The minimal inhibitory concentrations and biofilm inhibitory concentrations of commonly used antibiotics were determined. Results: 47 of 51 strains were resistant to three or more antibiotics. 42 of 51 strains formed Stenotrophomonas maltophilia biofilms in vitro. Stenotrophomonas maltophilia biofilm formation greatly reduced sensitivity to most tested antibiotics, but not to levofloxacin. However, in the presence of erythromycin scanning electron microscopy revealed that levofloxacin inhibited Stenotrophomonas maltophilia biofilm formation. Factorial ANOVA revealed that erythromycin enhanced susceptibility to levofloxacin, cefoperazone/sulbactam, and piperacillin (p < 0.05), and an ΔE model revealed that levofloxacin and erythromycin acted synergistically in biofilms, suggesting specific use of combined macrolide therapy may represent an effective treatment for Stenotrophomonas maltophilia infection. Conclusions: Antibiotics could act synergistically to combat the protection conferred to clinical isolates of Stenotrophomonas maltophilia by biofilms. Macrolide antibiotics may be effective where used in combination. Keywords: Stenotrophomonas maltophilia, Biofilm, Antibiotic resistance, Nosocomial |
| first_indexed | 2024-12-21T04:52:08Z |
| format | Article |
| id | doaj.art-a895a0c52c464606a0486743e54eca46 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-21T04:52:08Z |
| publishDate | 2016-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-a895a0c52c464606a0486743e54eca462022-12-21T19:15:25ZengElsevierBrazilian Journal of Infectious Diseases1413-86702016-07-01204365373S1413-86702016000400365Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilmsErlin Sun0Gehong Liang1Lining Wang2Wenjie Wei3Mingde Lei4Shiduo Song5Ruifa Han6Yubao Wang7Wei Qi8The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, China; Corresponding authors.The Second Hospital of Tianjin Medical University, Department of Infectious Disease, Tianjin, ChinaThe Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, ChinaUniversity of Pennsylvania, Department of Anatomy and Cell Biology, Philadelphia, PA, United StatesThe Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, ChinaThe Second Hospital of Tianjin Medical University, Department of Infectious Disease, Tianjin, ChinaThe Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, ChinaThe Second Hospital of Tianjin Medical University, Department of Infectious Disease, Tianjin, ChinaThe Second Hospital of Tianjin Medical University, Department of Infectious Disease, Tianjin, China; Corresponding authors.Aims: We sought to characterize the antibiotic susceptibility of strains of Stenotrophomonas maltophilia isolated from clinical samples, and the role of Stenotrophomonas maltophilia biofilm in antibiotic resistance. Methods: Fifty-one clinical Stenotrophomonas maltophilia isolates were obtained from patients with nosocomial infection in the surgical wards and ICUs of six general hospitals in Tianjin, China. In vitro models of Stenotrophomonas maltophilia biofilms were established and confirmed by scanning electron microscopy and fluorescence microscopy with silver staining. The minimal inhibitory concentrations and biofilm inhibitory concentrations of commonly used antibiotics were determined. Results: 47 of 51 strains were resistant to three or more antibiotics. 42 of 51 strains formed Stenotrophomonas maltophilia biofilms in vitro. Stenotrophomonas maltophilia biofilm formation greatly reduced sensitivity to most tested antibiotics, but not to levofloxacin. However, in the presence of erythromycin scanning electron microscopy revealed that levofloxacin inhibited Stenotrophomonas maltophilia biofilm formation. Factorial ANOVA revealed that erythromycin enhanced susceptibility to levofloxacin, cefoperazone/sulbactam, and piperacillin (p < 0.05), and an ΔE model revealed that levofloxacin and erythromycin acted synergistically in biofilms, suggesting specific use of combined macrolide therapy may represent an effective treatment for Stenotrophomonas maltophilia infection. Conclusions: Antibiotics could act synergistically to combat the protection conferred to clinical isolates of Stenotrophomonas maltophilia by biofilms. Macrolide antibiotics may be effective where used in combination. Keywords: Stenotrophomonas maltophilia, Biofilm, Antibiotic resistance, Nosocomialhttp://www.sciencedirect.com/science/article/pii/S1413867016300721 |
| spellingShingle | Erlin Sun Gehong Liang Lining Wang Wenjie Wei Mingde Lei Shiduo Song Ruifa Han Yubao Wang Wei Qi Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms Brazilian Journal of Infectious Diseases |
| title | Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms |
| title_full | Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms |
| title_fullStr | Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms |
| title_full_unstemmed | Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms |
| title_short | Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms |
| title_sort | antimicrobial susceptibility of hospital acquired stenotrophomonas maltophilia isolate biofilms |
| url | http://www.sciencedirect.com/science/article/pii/S1413867016300721 |
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