Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria
<p>Abstract</p> <p>Background</p> <p>GIMAP (GTPase of the immunity-associated protein family) proteins are a family of putative GTPases believed to be regulators of cell death in lymphomyeloid cells. GIMAP1 was the first reported member of this gene family, identified a...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-04-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/8/1/53 |
| _version_ | 1818957111684497408 |
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| author | Carter Christine Dion Carine Hutchings Amanda Pascall John Lamb Tracey Saunders Amy Hepburn Lucy Langhorne Jean Butcher Geoffrey W |
| author_facet | Carter Christine Dion Carine Hutchings Amanda Pascall John Lamb Tracey Saunders Amy Hepburn Lucy Langhorne Jean Butcher Geoffrey W |
| author_sort | Carter Christine |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>GIMAP (GTPase of the immunity-associated protein family) proteins are a family of putative GTPases believed to be regulators of cell death in lymphomyeloid cells. GIMAP1 was the first reported member of this gene family, identified as a gene up-regulated at the RNA level in the spleens of mice infected with the malarial parasite, <it>Plasmodium chabaudi</it>.</p> <p>Methods</p> <p>A monoclonal antibody against mouse GIMAP1 was developed and was used to analyse the expression of the endogenous protein in tissues of normal mice and in defined sub-populations of cells prepared from lymphoid tissues using flow cytometry. It was also used to assess the expression of GIMAP1 protein after infection and/or immunization of mice with <it>P. chabaudi</it>. Real-time PCR analysis was employed to measure the expression of <it>GIMAP1 </it>for comparison with the protein level analysis.</p> <p>Results</p> <p>GIMAP1 protein expression was detected in all lineages of lymphocytes (T, B, NK), in F4/80<sup>+ </sup>splenic macrophages and in some lymphoid cell lines. Additional evidence is presented suggesting that the strong expression by mature B cells of GIMAP1 and other GIMAP genes and proteins seen in mice may be a species-dependent characteristic. Unexpectedly, no increase was found in the expression of GIMAP1 in <it>P. chabaudi </it>infected mice at either the mRNA or protein level, and this remained so despite applying a number of variations to the protocol.</p> <p>Conclusion</p> <p>The model of up-regulation of GIMAP1 in response to infection/immunization with <it>P. chabaudi </it>is not a robustly reproducible experimental system. The GIMAP1 protein is widely expressed in lymphoid cells, with an interesting increase in expression in the later stages of B cell development. Alternative approaches will be required to define the functional role of this GTPase in immune cells.</p> |
| first_indexed | 2024-12-20T11:04:40Z |
| format | Article |
| id | doaj.art-a8a035b5124b4967a0290667ff740e21 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-20T11:04:40Z |
| publishDate | 2009-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-a8a035b5124b4967a0290667ff740e212022-12-21T19:42:54ZengBMCMalaria Journal1475-28752009-04-01815310.1186/1475-2875-8-53Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malariaCarter ChristineDion CarineHutchings AmandaPascall JohnLamb TraceySaunders AmyHepburn LucyLanghorne JeanButcher Geoffrey W<p>Abstract</p> <p>Background</p> <p>GIMAP (GTPase of the immunity-associated protein family) proteins are a family of putative GTPases believed to be regulators of cell death in lymphomyeloid cells. GIMAP1 was the first reported member of this gene family, identified as a gene up-regulated at the RNA level in the spleens of mice infected with the malarial parasite, <it>Plasmodium chabaudi</it>.</p> <p>Methods</p> <p>A monoclonal antibody against mouse GIMAP1 was developed and was used to analyse the expression of the endogenous protein in tissues of normal mice and in defined sub-populations of cells prepared from lymphoid tissues using flow cytometry. It was also used to assess the expression of GIMAP1 protein after infection and/or immunization of mice with <it>P. chabaudi</it>. Real-time PCR analysis was employed to measure the expression of <it>GIMAP1 </it>for comparison with the protein level analysis.</p> <p>Results</p> <p>GIMAP1 protein expression was detected in all lineages of lymphocytes (T, B, NK), in F4/80<sup>+ </sup>splenic macrophages and in some lymphoid cell lines. Additional evidence is presented suggesting that the strong expression by mature B cells of GIMAP1 and other GIMAP genes and proteins seen in mice may be a species-dependent characteristic. Unexpectedly, no increase was found in the expression of GIMAP1 in <it>P. chabaudi </it>infected mice at either the mRNA or protein level, and this remained so despite applying a number of variations to the protocol.</p> <p>Conclusion</p> <p>The model of up-regulation of GIMAP1 in response to infection/immunization with <it>P. chabaudi </it>is not a robustly reproducible experimental system. The GIMAP1 protein is widely expressed in lymphoid cells, with an interesting increase in expression in the later stages of B cell development. Alternative approaches will be required to define the functional role of this GTPase in immune cells.</p>http://www.malariajournal.com/content/8/1/53 |
| spellingShingle | Carter Christine Dion Carine Hutchings Amanda Pascall John Lamb Tracey Saunders Amy Hepburn Lucy Langhorne Jean Butcher Geoffrey W Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria Malaria Journal |
| title | Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria |
| title_full | Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria |
| title_fullStr | Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria |
| title_full_unstemmed | Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria |
| title_short | Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria |
| title_sort | expression of gimap1 a gtpase of the immunity associated protein family is not up regulated in malaria |
| url | http://www.malariajournal.com/content/8/1/53 |
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