Prolonged Activation of Brain CB2 Signaling Modulates Hypothalamic Microgliosis and Astrogliosis in High Fat Diet-Fed Mice
Low-grade inflammation of the hypothalamus is associated with the disturbance of energy balance. The endocannabinoid system has been implicated in the development and maintenance of obesity as well as in the control of immune responses. The type 2 cannabinoid receptor (CB2) signaling has been associ...
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5527 |
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| author | Rodrigo Rorato Nathalia L. Ferreira Franciane P. Oliveira Higor J. Fideles Tays A. Camilo Jose Antunes-Rodrigues Andre S. Mecawi Lucila L. K. Elias |
| author_facet | Rodrigo Rorato Nathalia L. Ferreira Franciane P. Oliveira Higor J. Fideles Tays A. Camilo Jose Antunes-Rodrigues Andre S. Mecawi Lucila L. K. Elias |
| author_sort | Rodrigo Rorato |
| collection | DOAJ |
| description | Low-grade inflammation of the hypothalamus is associated with the disturbance of energy balance. The endocannabinoid system has been implicated in the development and maintenance of obesity as well as in the control of immune responses. The type 2 cannabinoid receptor (CB2) signaling has been associated with anti-inflammatory effects. Therefore, in high fat diet (HFD)-induced obese mice, we modulated CB2 signaling and investigated its effects on energy homeostasis and hypothalamic microgliosis/astrogliosis. We observed no effect on caloric intake and body weight gain in control diet-fed animals that received prolonged icv infusion of the CB2 receptor agonist HU308. Interestingly, we observed a decrease in glucose tolerance in HFD-fed animals treated with HU308. Prolonged icv infusion of HU308 increases astrogliosis in the ventromedial nucleus (VMH) of obese animals and reduced HFD-induced microgliosis in the hypothalamic arcuate (ARC) but not in the paraventricular (PVN) or VMH nuclei. These data indicate that central CB2 signaling modulates glucose homeostasis and glial reactivity in obesogenic conditions, irrespective of changes in body weight. |
| first_indexed | 2024-03-10T03:44:42Z |
| format | Article |
| id | doaj.art-a8a0c6e83762426daee3fef06a448bdf |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:44:42Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a8a0c6e83762426daee3fef06a448bdf2023-11-23T11:24:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310552710.3390/ijms23105527Prolonged Activation of Brain CB2 Signaling Modulates Hypothalamic Microgliosis and Astrogliosis in High Fat Diet-Fed MiceRodrigo Rorato0Nathalia L. Ferreira1Franciane P. Oliveira2Higor J. Fideles3Tays A. Camilo4Jose Antunes-Rodrigues5Andre S. Mecawi6Lucila L. K. Elias7Laboratory of Stress Neuroendocrinology, Department of Biophysics, Paulista Medical School, Federal University of Sao Paulo, Sao Paulo 04023-062, BrazilLaboratory of Stress Neuroendocrinology, Department of Biophysics, Paulista Medical School, Federal University of Sao Paulo, Sao Paulo 04023-062, BrazilLaboratory of Stress Neuroendocrinology, Department of Biophysics, Paulista Medical School, Federal University of Sao Paulo, Sao Paulo 04023-062, BrazilLaboratory of Stress Neuroendocrinology, Department of Biophysics, Paulista Medical School, Federal University of Sao Paulo, Sao Paulo 04023-062, BrazilLaboratory of Molecular Neuroendocrinology, Department of Biophysics, Paulista Medical School, Federal University of Sao Paulo, Sao Paulo 04023-062, BrazilDepartment of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto 14049-900, BrazilLaboratory of Molecular Neuroendocrinology, Department of Biophysics, Paulista Medical School, Federal University of Sao Paulo, Sao Paulo 04023-062, BrazilDepartment of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto 14049-900, BrazilLow-grade inflammation of the hypothalamus is associated with the disturbance of energy balance. The endocannabinoid system has been implicated in the development and maintenance of obesity as well as in the control of immune responses. The type 2 cannabinoid receptor (CB2) signaling has been associated with anti-inflammatory effects. Therefore, in high fat diet (HFD)-induced obese mice, we modulated CB2 signaling and investigated its effects on energy homeostasis and hypothalamic microgliosis/astrogliosis. We observed no effect on caloric intake and body weight gain in control diet-fed animals that received prolonged icv infusion of the CB2 receptor agonist HU308. Interestingly, we observed a decrease in glucose tolerance in HFD-fed animals treated with HU308. Prolonged icv infusion of HU308 increases astrogliosis in the ventromedial nucleus (VMH) of obese animals and reduced HFD-induced microgliosis in the hypothalamic arcuate (ARC) but not in the paraventricular (PVN) or VMH nuclei. These data indicate that central CB2 signaling modulates glucose homeostasis and glial reactivity in obesogenic conditions, irrespective of changes in body weight.https://www.mdpi.com/1422-0067/23/10/5527obesityCB2 signalinghypothalamic gliosisglycaemia |
| spellingShingle | Rodrigo Rorato Nathalia L. Ferreira Franciane P. Oliveira Higor J. Fideles Tays A. Camilo Jose Antunes-Rodrigues Andre S. Mecawi Lucila L. K. Elias Prolonged Activation of Brain CB2 Signaling Modulates Hypothalamic Microgliosis and Astrogliosis in High Fat Diet-Fed Mice International Journal of Molecular Sciences obesity CB2 signaling hypothalamic gliosis glycaemia |
| title | Prolonged Activation of Brain CB2 Signaling Modulates Hypothalamic Microgliosis and Astrogliosis in High Fat Diet-Fed Mice |
| title_full | Prolonged Activation of Brain CB2 Signaling Modulates Hypothalamic Microgliosis and Astrogliosis in High Fat Diet-Fed Mice |
| title_fullStr | Prolonged Activation of Brain CB2 Signaling Modulates Hypothalamic Microgliosis and Astrogliosis in High Fat Diet-Fed Mice |
| title_full_unstemmed | Prolonged Activation of Brain CB2 Signaling Modulates Hypothalamic Microgliosis and Astrogliosis in High Fat Diet-Fed Mice |
| title_short | Prolonged Activation of Brain CB2 Signaling Modulates Hypothalamic Microgliosis and Astrogliosis in High Fat Diet-Fed Mice |
| title_sort | prolonged activation of brain cb2 signaling modulates hypothalamic microgliosis and astrogliosis in high fat diet fed mice |
| topic | obesity CB2 signaling hypothalamic gliosis glycaemia |
| url | https://www.mdpi.com/1422-0067/23/10/5527 |
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