Development and validation of a predictive model of the impact of single nucleotide polymorphisms in the ICAM-1 gene on the risk of ischemic cardiomyopathy
ObjectivePrevious research has linked single nucleotide polymorphisms (SNPs) in the ICAM-1 gene to an increased risk of developing ischemic cardiomyopathy (ICM); however, a diagnostic model of ICM according to the ICAM-1 variant has not yet been developed. Therefore, this study aimed to explore the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2022.977340/full |
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| author | Tuersunjiang Naman Refukaiti Abuduhalike Mubalake Yakufu Ayixigu Bawudun Juan Sun Ailiman Mahemuti |
| author_facet | Tuersunjiang Naman Refukaiti Abuduhalike Mubalake Yakufu Ayixigu Bawudun Juan Sun Ailiman Mahemuti |
| author_sort | Tuersunjiang Naman |
| collection | DOAJ |
| description | ObjectivePrevious research has linked single nucleotide polymorphisms (SNPs) in the ICAM-1 gene to an increased risk of developing ischemic cardiomyopathy (ICM); however, a diagnostic model of ICM according to the ICAM-1 variant has not yet been developed. Therefore, this study aimed to explore the correlation between SNPs in ICAM-1 and the presence of ICM, along with developing a diagnostic model for ICM based on the variants of the ICAM-1 gene.MethodThis study recruited a total of 252 patients with ICM and 280 healthy controls. In addition, all the participants were genotyped for SNPs in the ICAM-1 gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Using the training dataset of 371 people, we constructed a nomogram model based on ICAM-1 gene variants and clinical variables. To optimize the feature choice for the ICM risk model, a least absolute shrinkage and selection operator (LASSO) regression model was adopted. We also employed multivariable logistic regression analysis to build a prediction model by integrating the clinical characteristics chosen in the LASSO regression model. Following the receiver operating characteristic (ROC), a calibration plot and decision curve analysis (DCA) were used to evaluate the discrimination, calibration, and clinical usefulness of the predictive model.ResultThe predictors involved in the prediction nomogram included age, smoking, diabetes, low-density lipoprotein-cholesterol, hemoglobin, N-terminal pro-B-type natriuretic peptide, ejection fraction, and the rs5491 SNP. The nomogram model exhibited good discrimination ability, with the AUC value of ROC of 0.978 (95%CI: 0.967–0.989, P < 0.001) in the training group and 0.983 (95% CI: 0.969–0.998, P < 0.001) in the validation group. The Hosmer–Lemeshow test demonstrated good model calibration with consistency (Ptraining group = 0.937; Pvalidation group = 0.910). The DCA showed that the ICM nomogram was clinically beneficial, with the threshold probabilities ranging from 0.0 to 1.0.ConclusionThe AT genotype in rs5491 of the ICAM-1 gene was associated with having a higher frequency of ICM. Individuals carrying the mutant AT genotype showed a 5.816-fold higher frequency of ICM compared with those with the AA genotype. ICM patients with the AT genotype also had a higher rate of cardiogenic death. We, therefore, developed a nomogram model that could offer an individualized prediction of ICM risk factors. |
| first_indexed | 2024-04-12T11:09:24Z |
| format | Article |
| id | doaj.art-a8a15549ed124874b73059e271d79daf |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-12T11:09:24Z |
| publishDate | 2022-11-01 |
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| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-a8a15549ed124874b73059e271d79daf2022-12-22T03:35:39ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-11-01910.3389/fcvm.2022.977340977340Development and validation of a predictive model of the impact of single nucleotide polymorphisms in the ICAM-1 gene on the risk of ischemic cardiomyopathyTuersunjiang NamanRefukaiti AbuduhalikeMubalake YakufuAyixigu BawudunJuan SunAiliman MahemutiObjectivePrevious research has linked single nucleotide polymorphisms (SNPs) in the ICAM-1 gene to an increased risk of developing ischemic cardiomyopathy (ICM); however, a diagnostic model of ICM according to the ICAM-1 variant has not yet been developed. Therefore, this study aimed to explore the correlation between SNPs in ICAM-1 and the presence of ICM, along with developing a diagnostic model for ICM based on the variants of the ICAM-1 gene.MethodThis study recruited a total of 252 patients with ICM and 280 healthy controls. In addition, all the participants were genotyped for SNPs in the ICAM-1 gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Using the training dataset of 371 people, we constructed a nomogram model based on ICAM-1 gene variants and clinical variables. To optimize the feature choice for the ICM risk model, a least absolute shrinkage and selection operator (LASSO) regression model was adopted. We also employed multivariable logistic regression analysis to build a prediction model by integrating the clinical characteristics chosen in the LASSO regression model. Following the receiver operating characteristic (ROC), a calibration plot and decision curve analysis (DCA) were used to evaluate the discrimination, calibration, and clinical usefulness of the predictive model.ResultThe predictors involved in the prediction nomogram included age, smoking, diabetes, low-density lipoprotein-cholesterol, hemoglobin, N-terminal pro-B-type natriuretic peptide, ejection fraction, and the rs5491 SNP. The nomogram model exhibited good discrimination ability, with the AUC value of ROC of 0.978 (95%CI: 0.967–0.989, P < 0.001) in the training group and 0.983 (95% CI: 0.969–0.998, P < 0.001) in the validation group. The Hosmer–Lemeshow test demonstrated good model calibration with consistency (Ptraining group = 0.937; Pvalidation group = 0.910). The DCA showed that the ICM nomogram was clinically beneficial, with the threshold probabilities ranging from 0.0 to 1.0.ConclusionThe AT genotype in rs5491 of the ICAM-1 gene was associated with having a higher frequency of ICM. Individuals carrying the mutant AT genotype showed a 5.816-fold higher frequency of ICM compared with those with the AA genotype. ICM patients with the AT genotype also had a higher rate of cardiogenic death. We, therefore, developed a nomogram model that could offer an individualized prediction of ICM risk factors.https://www.frontiersin.org/articles/10.3389/fcvm.2022.977340/fullgene polymorphismvariants of ICAM-1 geneischemic cardiomyopathy (ICM)predictive modelrisk factors of ICM |
| spellingShingle | Tuersunjiang Naman Refukaiti Abuduhalike Mubalake Yakufu Ayixigu Bawudun Juan Sun Ailiman Mahemuti Development and validation of a predictive model of the impact of single nucleotide polymorphisms in the ICAM-1 gene on the risk of ischemic cardiomyopathy Frontiers in Cardiovascular Medicine gene polymorphism variants of ICAM-1 gene ischemic cardiomyopathy (ICM) predictive model risk factors of ICM |
| title | Development and validation of a predictive model of the impact of single nucleotide polymorphisms in the ICAM-1 gene on the risk of ischemic cardiomyopathy |
| title_full | Development and validation of a predictive model of the impact of single nucleotide polymorphisms in the ICAM-1 gene on the risk of ischemic cardiomyopathy |
| title_fullStr | Development and validation of a predictive model of the impact of single nucleotide polymorphisms in the ICAM-1 gene on the risk of ischemic cardiomyopathy |
| title_full_unstemmed | Development and validation of a predictive model of the impact of single nucleotide polymorphisms in the ICAM-1 gene on the risk of ischemic cardiomyopathy |
| title_short | Development and validation of a predictive model of the impact of single nucleotide polymorphisms in the ICAM-1 gene on the risk of ischemic cardiomyopathy |
| title_sort | development and validation of a predictive model of the impact of single nucleotide polymorphisms in the icam 1 gene on the risk of ischemic cardiomyopathy |
| topic | gene polymorphism variants of ICAM-1 gene ischemic cardiomyopathy (ICM) predictive model risk factors of ICM |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2022.977340/full |
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