Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil
The purpose of the study was to classify, through phylogenetic analyses, the main arboviruses that have been isolated in the metropolitan region of Porto Velho, Rondônia, Brazil. Serum samples from patients with symptoms suggesting arboviruses were collected and tested by One Step RT-qPCR for Zika,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/8/889 |
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| author | Jackson Alves da Silva Queiroz Luan Felipo Botelho-Souza Felipe Souza Nogueira-Lima Rita de Cássia Pontello Rampazzo Marco Aurélio Krieger Miriam Ribas Zambenedetti Fabricio Klerinton Marchini Ivo Alberto Borghetti Dhelio Batista Pereira Juan Miguel Vilalobos Salcedo Deusilene Souza Vieira Alcione de Oliveira dos Santos |
| author_facet | Jackson Alves da Silva Queiroz Luan Felipo Botelho-Souza Felipe Souza Nogueira-Lima Rita de Cássia Pontello Rampazzo Marco Aurélio Krieger Miriam Ribas Zambenedetti Fabricio Klerinton Marchini Ivo Alberto Borghetti Dhelio Batista Pereira Juan Miguel Vilalobos Salcedo Deusilene Souza Vieira Alcione de Oliveira dos Santos |
| author_sort | Jackson Alves da Silva Queiroz |
| collection | DOAJ |
| description | The purpose of the study was to classify, through phylogenetic analyses, the main arboviruses that have been isolated in the metropolitan region of Porto Velho, Rondônia, Brazil. Serum samples from patients with symptoms suggesting arboviruses were collected and tested by One Step RT-qPCR for Zika, Dengue (serotypes 1–4), Chikungunya, Mayaro and Oropouche viruses. Positive samples were amplified by conventional PCR and sequenced utilizing the Sanger method. The obtained sequences were aligned, and an evolutionary analysis was carried out using Bayesian inference. A total of 308 samples were tested. Of this total, 20 had a detectable viral load for Dengue, being detected DENV1 (18/20), co-infection DENV1 and DENV2 (1/20) and DENV4 (1/20). For Dengue serotype 3 and for the CHIKV, ZIKV, MAYV and OROV viruses, no individuals with a detectable viral load were found. A total of 9 of these samples were magnified by conventional PCR for sequencing. Of these, 6 were successfully sequenced and, according to the evolutionary profile, 5 corresponded to serotype DENV-1 genotype V, and 1 to serotype DENV-4 genotype II. In the study, we demonstrate co-circulation of the DENV-1 genotype V and the DENV-4 genotype II. Co-circulation of several DENV serotypes in the same city poses a risk to the population and is correlated with the increase of the most severe forms of the disease. Similarly, co-circulation of genetically distinct DENV and the occurrence of simultaneous infections can affect recombination events and lead to the emergence of more virulent isolates. |
| first_indexed | 2024-03-10T17:28:41Z |
| format | Article |
| id | doaj.art-a8a33ebd64674c89843da7d302a7191c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T17:28:41Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a8a33ebd64674c89843da7d302a7191c2023-11-20T10:06:51ZengMDPI AGViruses1999-49152020-08-0112888910.3390/v12080889Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, BrazilJackson Alves da Silva Queiroz0Luan Felipo Botelho-Souza1Felipe Souza Nogueira-Lima2Rita de Cássia Pontello Rampazzo3Marco Aurélio Krieger4Miriam Ribas Zambenedetti5Fabricio Klerinton Marchini6Ivo Alberto Borghetti7Dhelio Batista Pereira8Juan Miguel Vilalobos Salcedo9Deusilene Souza Vieira10Alcione de Oliveira dos Santos11Oswaldo Cruz Foundation of Rondônia—FIOCRUZ/RO, Porto Velho RO 76812 245, Rondônia, BrazilOswaldo Cruz Foundation of Rondônia—FIOCRUZ/RO, Porto Velho RO 76812 245, Rondônia, BrazilOswaldo Cruz Foundation of Rondônia—FIOCRUZ/RO, Porto Velho RO 76812 245, Rondônia, BrazilInstitute of Molecular Biology of Paraná -IBMP, Curitiba PR 81350-010, Rondônia, BrazilInstitute of Molecular Biology of Paraná -IBMP, Curitiba PR 81350-010, Rondônia, BrazilInstitute of Molecular Biology of Paraná -IBMP, Curitiba PR 81350-010, Rondônia, BrazilInstitute of Molecular Biology of Paraná -IBMP, Curitiba PR 81350-010, Rondônia, BrazilInstitute of Molecular Biology of Paraná -IBMP, Curitiba PR 81350-010, Rondônia, BrazilTropical Medicine of Rondônia Center Research—CEPEM/RO, Porto Velho RO 76812 329, Rondônia, BrazilOswaldo Cruz Foundation of Rondônia—FIOCRUZ/RO, Porto Velho RO 76812 245, Rondônia, BrazilOswaldo Cruz Foundation of Rondônia—FIOCRUZ/RO, Porto Velho RO 76812 245, Rondônia, BrazilOswaldo Cruz Foundation of Rondônia—FIOCRUZ/RO, Porto Velho RO 76812 245, Rondônia, BrazilThe purpose of the study was to classify, through phylogenetic analyses, the main arboviruses that have been isolated in the metropolitan region of Porto Velho, Rondônia, Brazil. Serum samples from patients with symptoms suggesting arboviruses were collected and tested by One Step RT-qPCR for Zika, Dengue (serotypes 1–4), Chikungunya, Mayaro and Oropouche viruses. Positive samples were amplified by conventional PCR and sequenced utilizing the Sanger method. The obtained sequences were aligned, and an evolutionary analysis was carried out using Bayesian inference. A total of 308 samples were tested. Of this total, 20 had a detectable viral load for Dengue, being detected DENV1 (18/20), co-infection DENV1 and DENV2 (1/20) and DENV4 (1/20). For Dengue serotype 3 and for the CHIKV, ZIKV, MAYV and OROV viruses, no individuals with a detectable viral load were found. A total of 9 of these samples were magnified by conventional PCR for sequencing. Of these, 6 were successfully sequenced and, according to the evolutionary profile, 5 corresponded to serotype DENV-1 genotype V, and 1 to serotype DENV-4 genotype II. In the study, we demonstrate co-circulation of the DENV-1 genotype V and the DENV-4 genotype II. Co-circulation of several DENV serotypes in the same city poses a risk to the population and is correlated with the increase of the most severe forms of the disease. Similarly, co-circulation of genetically distinct DENV and the occurrence of simultaneous infections can affect recombination events and lead to the emergence of more virulent isolates.https://www.mdpi.com/1999-4915/12/8/889arbovirusesphylogeneticgenotyping |
| spellingShingle | Jackson Alves da Silva Queiroz Luan Felipo Botelho-Souza Felipe Souza Nogueira-Lima Rita de Cássia Pontello Rampazzo Marco Aurélio Krieger Miriam Ribas Zambenedetti Fabricio Klerinton Marchini Ivo Alberto Borghetti Dhelio Batista Pereira Juan Miguel Vilalobos Salcedo Deusilene Souza Vieira Alcione de Oliveira dos Santos Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil Viruses arboviruses phylogenetic genotyping |
| title | Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil |
| title_full | Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil |
| title_fullStr | Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil |
| title_full_unstemmed | Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil |
| title_short | Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil |
| title_sort | phylogenetic characterization of arboviruses in patients suffering from acute fever in rondonia brazil |
| topic | arboviruses phylogenetic genotyping |
| url | https://www.mdpi.com/1999-4915/12/8/889 |
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