Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells
<p>Abstract</p> <p>Exhaled nitric oxide (eNO) is decreased by cigarette smoking. The hypothesis that oxides of nitrogen (NO<sub>X</sub>) in cigarette smoke solution (CSS) may exert a negative feedback mechanism upon NO release from epithelial (AEC, A549, and NHTBE) and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2005-09-01
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| Series: | Respiratory Research |
| Subjects: | |
| Online Access: | http://respiratory-research.com/content/6/1/108 |
| _version_ | 1818141136533848064 |
|---|---|
| author | Hunt John E Heywood Gavin J Kumar Rakesh K Kim Henry S Wei Xiu M McNeil H Patrick Thomas Paul S |
| author_facet | Hunt John E Heywood Gavin J Kumar Rakesh K Kim Henry S Wei Xiu M McNeil H Patrick Thomas Paul S |
| author_sort | Hunt John E |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Exhaled nitric oxide (eNO) is decreased by cigarette smoking. The hypothesis that oxides of nitrogen (NO<sub>X</sub>) in cigarette smoke solution (CSS) may exert a negative feedback mechanism upon NO release from epithelial (AEC, A549, and NHTBE) and basophilic cells (RBL-2H3) was tested in vitro. CSS inhibited both NO production and degranulation (measured as release of beta-hexosaminidase) in a dose-dependent manner from RBL-2H3 cells. Inhibition of NO production by CSS in AEC, A549, and NHTBE cells was also dose-dependent. In addition, CSS decreased expression of NOS mRNA and protein expression. The addition of NO inhibitors and scavengers did not, however, reverse the effects of CSS, nor did a NO donor (SNP) or nicotine mimic CSS. N-acetyl-cysteine, partially reversed the inhibition of beta-hexosaminidase release suggesting CSS may act via oxidative free radicals. Thus, some of the inhibitory effects of CSS appear to be via oxidative free radicals rather than a NO<sub>X </sub>-related negative feedback.</p> |
| first_indexed | 2024-12-11T10:55:05Z |
| format | Article |
| id | doaj.art-a8a4bce19da64589a829b19748e9e45a |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-12-11T10:55:05Z |
| publishDate | 2005-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-a8a4bce19da64589a829b19748e9e45a2022-12-22T01:10:08ZengBMCRespiratory Research1465-99212005-09-016110810.1186/1465-9921-6-108Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cellsHunt John EHeywood Gavin JKumar Rakesh KKim Henry SWei Xiu MMcNeil H PatrickThomas Paul S<p>Abstract</p> <p>Exhaled nitric oxide (eNO) is decreased by cigarette smoking. The hypothesis that oxides of nitrogen (NO<sub>X</sub>) in cigarette smoke solution (CSS) may exert a negative feedback mechanism upon NO release from epithelial (AEC, A549, and NHTBE) and basophilic cells (RBL-2H3) was tested in vitro. CSS inhibited both NO production and degranulation (measured as release of beta-hexosaminidase) in a dose-dependent manner from RBL-2H3 cells. Inhibition of NO production by CSS in AEC, A549, and NHTBE cells was also dose-dependent. In addition, CSS decreased expression of NOS mRNA and protein expression. The addition of NO inhibitors and scavengers did not, however, reverse the effects of CSS, nor did a NO donor (SNP) or nicotine mimic CSS. N-acetyl-cysteine, partially reversed the inhibition of beta-hexosaminidase release suggesting CSS may act via oxidative free radicals. Thus, some of the inhibitory effects of CSS appear to be via oxidative free radicals rather than a NO<sub>X </sub>-related negative feedback.</p>http://respiratory-research.com/content/6/1/108nitric oxidemast cellsepithelial cellscigarette smoke |
| spellingShingle | Hunt John E Heywood Gavin J Kumar Rakesh K Kim Henry S Wei Xiu M McNeil H Patrick Thomas Paul S Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells Respiratory Research nitric oxide mast cells epithelial cells cigarette smoke |
| title | Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells |
| title_full | Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells |
| title_fullStr | Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells |
| title_full_unstemmed | Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells |
| title_short | Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells |
| title_sort | effects of cigarette smoke on degranulation and no production by mast cells and epithelial cells |
| topic | nitric oxide mast cells epithelial cells cigarette smoke |
| url | http://respiratory-research.com/content/6/1/108 |
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