Detection and Characterization of ?-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China
To characterize the ?-lactam resistance in veterinary clinical isolates of Haemophilus parasuis, 115 isolates were examined for the ?-lactam resistance, the possession of ?-lactamase, and the presence of ?-lactamase genes. The genetic relationship among isolates was evaluated by pulsed-field gel ele...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-01-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1671292712607895 |
| _version_ | 1818822447344910336 |
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| author | Li-li GUO Jian-min ZHANG Cheng-gang XU Tao REN Bin ZHANG Ji-dang CHEN Ming LIAO |
| author_facet | Li-li GUO Jian-min ZHANG Cheng-gang XU Tao REN Bin ZHANG Ji-dang CHEN Ming LIAO |
| author_sort | Li-li GUO |
| collection | DOAJ |
| description | To characterize the ?-lactam resistance in veterinary clinical isolates of Haemophilus parasuis, 115 isolates were examined for the ?-lactam resistance, the possession of ?-lactamase, and the presence of ?-lactamase genes. The genetic relationship among isolates was evaluated by pulsed-field gel electrophoresis (PFGE). Overall, the commonly detected resistance phenotypes were resistant to ampicillin (26.09%), penicillin (22.61%), amoxicillin (21.74%), cefazolin (14.78%), cefaclor (12.17%), and cefotaxime (6.96%). These strains showed high minimal inhibitory concentration (MICs) to oxacillin. 20.87% strains produced ?-lactamase, and 4.35% strains showed extended-spectrum b-lactamase (ESBL) phenotype. Moreover, 19 strains harboured bla genes including TEM-1 (n=5), TEM-116 (n=10), and ROB-1 (n=5). Significantly, one strain possessed both TEM-1 and ROB-1, and displayed resistance to cefotaxime (MIC=8 mg L-1). The epidemiological analysis of PFGE revealed high genetic diversity among bla-positive isolates. This work shows that TEM- and ROB-type ?-lactamases are prevalent in H. parasuis isolates in China. |
| first_indexed | 2024-12-18T23:24:14Z |
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| id | doaj.art-a8a50ee2c7bb49c6b7364a4383e5f401 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-18T23:24:14Z |
| publishDate | 2012-01-01 |
| publisher | Elsevier |
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| series | Journal of Integrative Agriculture |
| spelling | doaj.art-a8a50ee2c7bb49c6b7364a4383e5f4012022-12-21T20:47:51ZengElsevierJournal of Integrative Agriculture2095-31192012-01-01111116121Detection and Characterization of ?-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South ChinaLi-li GUO0Jian-min ZHANG1Cheng-gang XU2Tao REN3Bin ZHANG4Ji-dang CHEN5Ming LIAO6GUO Li-li; Key Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R. ChinaKey Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R. ChinaKey Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R. ChinaKey Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R. ChinaKey Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R. ChinaKey Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R. ChinaCorrespondence LIAO Ming, Tel: +86-20-85280240, Fax: +86-20-85285282; Key Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R. ChinaTo characterize the ?-lactam resistance in veterinary clinical isolates of Haemophilus parasuis, 115 isolates were examined for the ?-lactam resistance, the possession of ?-lactamase, and the presence of ?-lactamase genes. The genetic relationship among isolates was evaluated by pulsed-field gel electrophoresis (PFGE). Overall, the commonly detected resistance phenotypes were resistant to ampicillin (26.09%), penicillin (22.61%), amoxicillin (21.74%), cefazolin (14.78%), cefaclor (12.17%), and cefotaxime (6.96%). These strains showed high minimal inhibitory concentration (MICs) to oxacillin. 20.87% strains produced ?-lactamase, and 4.35% strains showed extended-spectrum b-lactamase (ESBL) phenotype. Moreover, 19 strains harboured bla genes including TEM-1 (n=5), TEM-116 (n=10), and ROB-1 (n=5). Significantly, one strain possessed both TEM-1 and ROB-1, and displayed resistance to cefotaxime (MIC=8 mg L-1). The epidemiological analysis of PFGE revealed high genetic diversity among bla-positive isolates. This work shows that TEM- and ROB-type ?-lactamases are prevalent in H. parasuis isolates in China.http://www.sciencedirect.com/science/article/pii/S1671292712607895Haemophilus parasuis?-lactamase genesresistancepulsed-field gel electrophoresis |
| spellingShingle | Li-li GUO Jian-min ZHANG Cheng-gang XU Tao REN Bin ZHANG Ji-dang CHEN Ming LIAO Detection and Characterization of ?-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China Journal of Integrative Agriculture Haemophilus parasuis ?-lactamase genes resistance pulsed-field gel electrophoresis |
| title | Detection and Characterization of ?-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China |
| title_full | Detection and Characterization of ?-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China |
| title_fullStr | Detection and Characterization of ?-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China |
| title_full_unstemmed | Detection and Characterization of ?-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China |
| title_short | Detection and Characterization of ?-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China |
| title_sort | detection and characterization of lactam resistance in haemophilus parasuis strains from pigs in south china |
| topic | Haemophilus parasuis ?-lactamase genes resistance pulsed-field gel electrophoresis |
| url | http://www.sciencedirect.com/science/article/pii/S1671292712607895 |
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