Disposable Stochastic Sensors Based on Nanolayer Deposition(s) of Silver and AgC Composite on Plastic for the Assay of α-amylase in Whole Blood and Saliva
Nanolayer deposition(s) of Ag and AgC composite on a plastic substrate was used to design disposable stochastic sensors. Three shapes of nanocoatings were tested. The first shape was obtained by deposition of a nanofilm of Ag on the plastic material; the second shape was obtained by deposition of a...
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MDPI AG
2020-08-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/8/1528 |
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| author | Raluca-Ioana Stefan-van Staden Alexandrina Moscalu-Lungu Marius Badulescu |
| author_facet | Raluca-Ioana Stefan-van Staden Alexandrina Moscalu-Lungu Marius Badulescu |
| author_sort | Raluca-Ioana Stefan-van Staden |
| collection | DOAJ |
| description | Nanolayer deposition(s) of Ag and AgC composite on a plastic substrate was used to design disposable stochastic sensors. Three shapes of nanocoatings were tested. The first shape was obtained by deposition of a nanofilm of Ag on the plastic material; the second shape was obtained by deposition of a composite AgC nanolayer on the plastic material; the third shape was obtained by nanolayer deposition of a composite material of AgC on the top of the Ag nanofilm deposited on the plastic material. α-Cyclodextrin was used to modify the active surface of the sensor. Wide linear concentration ranges were obtained as follows: for de-assay of α-amylase in whole blood: 1.00 × 10<sup>−7</sup>–1.00 × 10<sup>3</sup> U mL<sup>−1</sup> and for the assay of α-amylase in saliva: 1.5 × 10<sup>−15</sup>–1.5 × 10<sup>2</sup> U mL<sup>−1</sup>. α-Amylase was reliably determined from whole blood and saliva samples using the proposed disposable stochastic sensors. |
| first_indexed | 2024-03-10T17:58:17Z |
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| id | doaj.art-a8a7d51045b14872a39bf9b3e431b31a |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T17:58:17Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-a8a7d51045b14872a39bf9b3e431b31a2023-11-20T09:04:12ZengMDPI AGNanomaterials2079-49912020-08-01108152810.3390/nano10081528Disposable Stochastic Sensors Based on Nanolayer Deposition(s) of Silver and AgC Composite on Plastic for the Assay of α-amylase in Whole Blood and SalivaRaluca-Ioana Stefan-van Staden0Alexandrina Moscalu-Lungu1Marius Badulescu2Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, 060021 Bucharest, RomaniaLaboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, 060021 Bucharest, RomaniaLow Temperature Plasma Laboratory, National Institute for Lasers, Plasma and Radiation Physics (NILPRP), 409 Atomistilor St., 077125 Magurele, RomaniaNanolayer deposition(s) of Ag and AgC composite on a plastic substrate was used to design disposable stochastic sensors. Three shapes of nanocoatings were tested. The first shape was obtained by deposition of a nanofilm of Ag on the plastic material; the second shape was obtained by deposition of a composite AgC nanolayer on the plastic material; the third shape was obtained by nanolayer deposition of a composite material of AgC on the top of the Ag nanofilm deposited on the plastic material. α-Cyclodextrin was used to modify the active surface of the sensor. Wide linear concentration ranges were obtained as follows: for de-assay of α-amylase in whole blood: 1.00 × 10<sup>−7</sup>–1.00 × 10<sup>3</sup> U mL<sup>−1</sup> and for the assay of α-amylase in saliva: 1.5 × 10<sup>−15</sup>–1.5 × 10<sup>2</sup> U mL<sup>−1</sup>. α-Amylase was reliably determined from whole blood and saliva samples using the proposed disposable stochastic sensors.https://www.mdpi.com/2079-4991/10/8/1528α-amylasedisposable stochastic sensorsnanolayer deposition(s)bioanalysis |
| spellingShingle | Raluca-Ioana Stefan-van Staden Alexandrina Moscalu-Lungu Marius Badulescu Disposable Stochastic Sensors Based on Nanolayer Deposition(s) of Silver and AgC Composite on Plastic for the Assay of α-amylase in Whole Blood and Saliva Nanomaterials α-amylase disposable stochastic sensors nanolayer deposition(s) bioanalysis |
| title | Disposable Stochastic Sensors Based on Nanolayer Deposition(s) of Silver and AgC Composite on Plastic for the Assay of α-amylase in Whole Blood and Saliva |
| title_full | Disposable Stochastic Sensors Based on Nanolayer Deposition(s) of Silver and AgC Composite on Plastic for the Assay of α-amylase in Whole Blood and Saliva |
| title_fullStr | Disposable Stochastic Sensors Based on Nanolayer Deposition(s) of Silver and AgC Composite on Plastic for the Assay of α-amylase in Whole Blood and Saliva |
| title_full_unstemmed | Disposable Stochastic Sensors Based on Nanolayer Deposition(s) of Silver and AgC Composite on Plastic for the Assay of α-amylase in Whole Blood and Saliva |
| title_short | Disposable Stochastic Sensors Based on Nanolayer Deposition(s) of Silver and AgC Composite on Plastic for the Assay of α-amylase in Whole Blood and Saliva |
| title_sort | disposable stochastic sensors based on nanolayer deposition s of silver and agc composite on plastic for the assay of α amylase in whole blood and saliva |
| topic | α-amylase disposable stochastic sensors nanolayer deposition(s) bioanalysis |
| url | https://www.mdpi.com/2079-4991/10/8/1528 |
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