Somatic embryogenesis and plantlet regeneration from nucleus tissues of Local orange (Citrus sinensis (L.) Osbeck)
Somatic embryogenesis and plantlet regeneration were achieved in callus cultures of nucellus tissues derived from undeveloped ovules of immature fruits of local orange (Citrus sinensis (L.) Osbeck). Four types of culture media were used and all produced embryogenic callus. Somatic embryos were deve...
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| Format: | Article |
| Language: | English |
| Published: |
University of Ljubljana Press (Založba Univerze v Ljubljani)
2012-10-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/14507 |
| _version_ | 1797390897959141376 |
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| author | Huda Abdel-karim Al-TAHA Abbas Mehdi JASIM Muayed Fadhil ABBAS |
| author_facet | Huda Abdel-karim Al-TAHA Abbas Mehdi JASIM Muayed Fadhil ABBAS |
| author_sort | Huda Abdel-karim Al-TAHA |
| collection | DOAJ |
| description |
Somatic embryogenesis and plantlet regeneration were achieved in callus cultures of nucellus tissues derived from undeveloped ovules of immature fruits of local orange (Citrus sinensis (L.) Osbeck). Four types of culture media were used and all produced embryogenic callus. Somatic embryos were developed on MS medium supplemented with BA only. Embryo maturation took place on MS medium supplemented with BA and 2,4-D. Plantlets were regenerated from those somatic embryos on half strength MS medium free of plant growth regulators. The well-developed plantlets were transferred to a potting mix containing sand and peat moss (2: 1) and grown for 8 months, with a survival rate of 100 %.
Abbreviation: BA: 6-benzyl amino purine; 2,4-D: 2,4-dichlorophenoxy acetic acid; NAA: α-naphthalene acetic acid
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| first_indexed | 2024-03-08T23:24:04Z |
| format | Article |
| id | doaj.art-a8aa524fc8b9472584686e45dd492be8 |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2024-03-08T23:24:04Z |
| publishDate | 2012-10-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-a8aa524fc8b9472584686e45dd492be82023-12-14T22:14:21ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412012-10-01992Somatic embryogenesis and plantlet regeneration from nucleus tissues of Local orange (Citrus sinensis (L.) Osbeck)Huda Abdel-karim Al-TAHA0Abbas Mehdi JASIM1Muayed Fadhil ABBAS2College of Agriculture, Basrah University, BasrahCollege of Agriculture, Basrah University, BasrahCollege of Agriculture, Basrah University, Basrah Somatic embryogenesis and plantlet regeneration were achieved in callus cultures of nucellus tissues derived from undeveloped ovules of immature fruits of local orange (Citrus sinensis (L.) Osbeck). Four types of culture media were used and all produced embryogenic callus. Somatic embryos were developed on MS medium supplemented with BA only. Embryo maturation took place on MS medium supplemented with BA and 2,4-D. Plantlets were regenerated from those somatic embryos on half strength MS medium free of plant growth regulators. The well-developed plantlets were transferred to a potting mix containing sand and peat moss (2: 1) and grown for 8 months, with a survival rate of 100 %. Abbreviation: BA: 6-benzyl amino purine; 2,4-D: 2,4-dichlorophenoxy acetic acid; NAA: α-naphthalene acetic acid https://journals.uni-lj.si/aas/article/view/14507Citrus sinensisembryogenic callussomatic embryogenesisnucleus tissuesplantlet regeneration |
| spellingShingle | Huda Abdel-karim Al-TAHA Abbas Mehdi JASIM Muayed Fadhil ABBAS Somatic embryogenesis and plantlet regeneration from nucleus tissues of Local orange (Citrus sinensis (L.) Osbeck) Acta Agriculturae Slovenica Citrus sinensis embryogenic callus somatic embryogenesis nucleus tissues plantlet regeneration |
| title | Somatic embryogenesis and plantlet regeneration from nucleus tissues of Local orange (Citrus sinensis (L.) Osbeck) |
| title_full | Somatic embryogenesis and plantlet regeneration from nucleus tissues of Local orange (Citrus sinensis (L.) Osbeck) |
| title_fullStr | Somatic embryogenesis and plantlet regeneration from nucleus tissues of Local orange (Citrus sinensis (L.) Osbeck) |
| title_full_unstemmed | Somatic embryogenesis and plantlet regeneration from nucleus tissues of Local orange (Citrus sinensis (L.) Osbeck) |
| title_short | Somatic embryogenesis and plantlet regeneration from nucleus tissues of Local orange (Citrus sinensis (L.) Osbeck) |
| title_sort | somatic embryogenesis and plantlet regeneration from nucleus tissues of local orange citrus sinensis l osbeck |
| topic | Citrus sinensis embryogenic callus somatic embryogenesis nucleus tissues plantlet regeneration |
| url | https://journals.uni-lj.si/aas/article/view/14507 |
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