Population genetic structure, migration, and polyploidy origin of a medicinal species Gynostemma pentaphyllum (Cucurbitaceae)
Abstract Gynostemma pentaphyllum, a member of family Cucurbitaceae, is a perennial creeping herb used as a traditional medicinal plant in China. In this study, six polymorphic nSSR and four EST‐SSR primers were used to genotype 1,020 individuals in 72 wild populations of G. pentaphyllum. The genetic...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-10-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.5618 |
| _version_ | 1818719932660056064 |
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| author | Xiao Zhang Hailun Su Jia Yang Li Feng Zhonghu Li Guifang Zhao |
| author_facet | Xiao Zhang Hailun Su Jia Yang Li Feng Zhonghu Li Guifang Zhao |
| author_sort | Xiao Zhang |
| collection | DOAJ |
| description | Abstract Gynostemma pentaphyllum, a member of family Cucurbitaceae, is a perennial creeping herb used as a traditional medicinal plant in China. In this study, six polymorphic nSSR and four EST‐SSR primers were used to genotype 1,020 individuals in 72 wild populations of G. pentaphyllum. The genetic diversity and population structure were investigated, and ecological niche modeling was performed to reveal the evolution and demographic history of its natural populations. The results show that G. pentaphyllum has a low level of genetic diversity and high level of variation among populations because of pervasive asexual propagation, genetic drift, and long‐term habitat isolation. Results of the Mantel test demonstrate that the genetic distance and geographic distance are significantly correlated among G. pentaphyllum natural populations. The populations can be divided into two clusters on the basis of genetic structure. Asymmetrical patterns of historical gene flow were observed among the clusters. For the contemporary, almost all the bidirectional gene flow of the related pairs was symmetrical with slight differences. Recent bottlenecks were experienced by 34.72% of the studied populations. The geographic range of G. pentaphyllum continues to expand northward and eastward from Hengduan Mountains. The present distribution of G. pentaphyllum is a consequence of its complex evolution. Polyploidy in G. pentaphyllum is inferred to be polygenetic. Finally, G. pentaphyllum is a species in need of protection, so in situ and ex situ measures should be considered in the future. |
| first_indexed | 2024-12-17T20:14:48Z |
| format | Article |
| id | doaj.art-a8ab9d2dbd854b50bcbbcf5b7cc39953 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-12-17T20:14:48Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-a8ab9d2dbd854b50bcbbcf5b7cc399532022-12-21T21:34:08ZengWileyEcology and Evolution2045-77582019-10-01919111451117010.1002/ece3.5618Population genetic structure, migration, and polyploidy origin of a medicinal species Gynostemma pentaphyllum (Cucurbitaceae)Xiao Zhang0Hailun Su1Jia Yang2Li Feng3Zhonghu Li4Guifang Zhao5Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education) College of Life Sciences Northwest University Xi'an ChinaKey Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education) College of Life Sciences Northwest University Xi'an ChinaKey Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education) College of Life Sciences Northwest University Xi'an ChinaKey Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education) College of Life Sciences Northwest University Xi'an ChinaKey Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education) College of Life Sciences Northwest University Xi'an ChinaKey Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education) College of Life Sciences Northwest University Xi'an ChinaAbstract Gynostemma pentaphyllum, a member of family Cucurbitaceae, is a perennial creeping herb used as a traditional medicinal plant in China. In this study, six polymorphic nSSR and four EST‐SSR primers were used to genotype 1,020 individuals in 72 wild populations of G. pentaphyllum. The genetic diversity and population structure were investigated, and ecological niche modeling was performed to reveal the evolution and demographic history of its natural populations. The results show that G. pentaphyllum has a low level of genetic diversity and high level of variation among populations because of pervasive asexual propagation, genetic drift, and long‐term habitat isolation. Results of the Mantel test demonstrate that the genetic distance and geographic distance are significantly correlated among G. pentaphyllum natural populations. The populations can be divided into two clusters on the basis of genetic structure. Asymmetrical patterns of historical gene flow were observed among the clusters. For the contemporary, almost all the bidirectional gene flow of the related pairs was symmetrical with slight differences. Recent bottlenecks were experienced by 34.72% of the studied populations. The geographic range of G. pentaphyllum continues to expand northward and eastward from Hengduan Mountains. The present distribution of G. pentaphyllum is a consequence of its complex evolution. Polyploidy in G. pentaphyllum is inferred to be polygenetic. Finally, G. pentaphyllum is a species in need of protection, so in situ and ex situ measures should be considered in the future.https://doi.org/10.1002/ece3.5618conservation strategygenetic diversityGynostemma pentaphyllummigrationpolyploidy originpopulation structure |
| spellingShingle | Xiao Zhang Hailun Su Jia Yang Li Feng Zhonghu Li Guifang Zhao Population genetic structure, migration, and polyploidy origin of a medicinal species Gynostemma pentaphyllum (Cucurbitaceae) Ecology and Evolution conservation strategy genetic diversity Gynostemma pentaphyllum migration polyploidy origin population structure |
| title | Population genetic structure, migration, and polyploidy origin of a medicinal species Gynostemma pentaphyllum (Cucurbitaceae) |
| title_full | Population genetic structure, migration, and polyploidy origin of a medicinal species Gynostemma pentaphyllum (Cucurbitaceae) |
| title_fullStr | Population genetic structure, migration, and polyploidy origin of a medicinal species Gynostemma pentaphyllum (Cucurbitaceae) |
| title_full_unstemmed | Population genetic structure, migration, and polyploidy origin of a medicinal species Gynostemma pentaphyllum (Cucurbitaceae) |
| title_short | Population genetic structure, migration, and polyploidy origin of a medicinal species Gynostemma pentaphyllum (Cucurbitaceae) |
| title_sort | population genetic structure migration and polyploidy origin of a medicinal species gynostemma pentaphyllum cucurbitaceae |
| topic | conservation strategy genetic diversity Gynostemma pentaphyllum migration polyploidy origin population structure |
| url | https://doi.org/10.1002/ece3.5618 |
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