Effects of lime and oxalic acid on antioxidant enzymes and active components of Panax notoginseng under cadmium stress
Abstract Cadmium (Cd) pollution poses potential safety risks for Panax notoginseng cultivation, a medicinal plant in Yunnan. Under exogenous Cd stress, field experiments were conducted to understand the effects of lime (0, 750, 2250 and 3750 kg hm−2) applied and oxalic acid (0, 0.1 and 0.2 mol L−1)...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-15280-w |
| _version_ | 1828775076605984768 |
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| author | Qi Li Na Jiang Xinyue Mei Yanqun Zu Zuran Li Li Qin Bo Li |
| author_facet | Qi Li Na Jiang Xinyue Mei Yanqun Zu Zuran Li Li Qin Bo Li |
| author_sort | Qi Li |
| collection | DOAJ |
| description | Abstract Cadmium (Cd) pollution poses potential safety risks for Panax notoginseng cultivation, a medicinal plant in Yunnan. Under exogenous Cd stress, field experiments were conducted to understand the effects of lime (0, 750, 2250 and 3750 kg hm−2) applied and oxalic acid (0, 0.1 and 0.2 mol L−1) leaves sprayed on Cd accumulation, antioxidant system and medicinal components of P. notoginseng. The results showed that Lime and foliar spray of oxalic acid were able to elevate Ca2+ and alleviate Cd2+ toxicity in P. notoginseng under Cd stress. The addition of lime and oxalic acid increased the activities of antioxidant enzymes and alters osmoregulator metabolism. The most significant increase in CAT activities increased by 2.77 folds. And the highest increase of SOD activities was 1.78 folds under the application of oxalic acid. While MDA content decreased by 58.38%. There were very significant correlation with soluble sugar, free amino acid, proline and soluble protein. Lime and oxalic acid were able to increase calcium ions (Ca2+), decrease Cd content and improve the stress resistance of P. notoginseng, while increasing the production of total saponins and flavonoids. Cd content were the lowest, 68.57% lower than controls, and met the standard value (Cd ≤ 0.5 mg kg−1, GB/T 19086-2008). The proportion of SPN was 7.73%, which reached the highest level of all treatments, the flavonoids content increased significantly by 21.74%, which reached the medicinal standard value and optimal yield. |
| first_indexed | 2024-12-11T15:32:44Z |
| format | Article |
| id | doaj.art-a8abd10888904d5ab9c4f6ba733a2c1a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-11T15:32:44Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a8abd10888904d5ab9c4f6ba733a2c1a2022-12-22T01:00:01ZengNature PortfolioScientific Reports2045-23222022-07-0112111310.1038/s41598-022-15280-wEffects of lime and oxalic acid on antioxidant enzymes and active components of Panax notoginseng under cadmium stressQi Li0Na Jiang1Xinyue Mei2Yanqun Zu3Zuran Li4Li Qin5Bo Li6College of Resources and Environment, Yunnan Agricultural UniversityCollege of Resources and Environment, Yunnan Agricultural UniversityCollege of Resources and Environment, Yunnan Agricultural UniversityCollege of Resources and Environment, Yunnan Agricultural UniversityCollege of Landscape and Horticulture, Yunnan Agricultural UniversityCollege of Resources and Environment, Yunnan Agricultural UniversityCollege of Resources and Environment, Yunnan Agricultural UniversityAbstract Cadmium (Cd) pollution poses potential safety risks for Panax notoginseng cultivation, a medicinal plant in Yunnan. Under exogenous Cd stress, field experiments were conducted to understand the effects of lime (0, 750, 2250 and 3750 kg hm−2) applied and oxalic acid (0, 0.1 and 0.2 mol L−1) leaves sprayed on Cd accumulation, antioxidant system and medicinal components of P. notoginseng. The results showed that Lime and foliar spray of oxalic acid were able to elevate Ca2+ and alleviate Cd2+ toxicity in P. notoginseng under Cd stress. The addition of lime and oxalic acid increased the activities of antioxidant enzymes and alters osmoregulator metabolism. The most significant increase in CAT activities increased by 2.77 folds. And the highest increase of SOD activities was 1.78 folds under the application of oxalic acid. While MDA content decreased by 58.38%. There were very significant correlation with soluble sugar, free amino acid, proline and soluble protein. Lime and oxalic acid were able to increase calcium ions (Ca2+), decrease Cd content and improve the stress resistance of P. notoginseng, while increasing the production of total saponins and flavonoids. Cd content were the lowest, 68.57% lower than controls, and met the standard value (Cd ≤ 0.5 mg kg−1, GB/T 19086-2008). The proportion of SPN was 7.73%, which reached the highest level of all treatments, the flavonoids content increased significantly by 21.74%, which reached the medicinal standard value and optimal yield.https://doi.org/10.1038/s41598-022-15280-w |
| spellingShingle | Qi Li Na Jiang Xinyue Mei Yanqun Zu Zuran Li Li Qin Bo Li Effects of lime and oxalic acid on antioxidant enzymes and active components of Panax notoginseng under cadmium stress Scientific Reports |
| title | Effects of lime and oxalic acid on antioxidant enzymes and active components of Panax notoginseng under cadmium stress |
| title_full | Effects of lime and oxalic acid on antioxidant enzymes and active components of Panax notoginseng under cadmium stress |
| title_fullStr | Effects of lime and oxalic acid on antioxidant enzymes and active components of Panax notoginseng under cadmium stress |
| title_full_unstemmed | Effects of lime and oxalic acid on antioxidant enzymes and active components of Panax notoginseng under cadmium stress |
| title_short | Effects of lime and oxalic acid on antioxidant enzymes and active components of Panax notoginseng under cadmium stress |
| title_sort | effects of lime and oxalic acid on antioxidant enzymes and active components of panax notoginseng under cadmium stress |
| url | https://doi.org/10.1038/s41598-022-15280-w |
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