The Study of Paraoxonase 2, Lipid Profile, and Total Oxidative Stress in Ischemic Stroke
Background: Paraoxonase2 (PON2) is an intracellular protein and widely present in many tissues such as endothelial cell and heart. It plays a major role in prevention of low-density lipoprotein (LDL) oxidation and reverses mildly oxidized LDL. PON2 plays an important role in antioxidation, antiather...
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| Format: | Article |
| Language: | English |
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ADICHUNCHANAGIRI INSTITUTE OF MEDICAL SCIENCES
2020-10-01
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| Series: | Journal of Medical Sciences and Health |
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| Online Access: | https://jmsh.ac.in/index.php?option=com_k2&view=item&id=144:the-study-of-paraoxonase-2-lipid-profile-and-total-oxidative-stress-in-ischemic-stroke&Itemid=1 |
| _version_ | 1818205712668426240 |
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| author | Seema S Chawhan M R Mogarekar Rajesh K Jambhulkar Sanjay M Chawhan |
| author_facet | Seema S Chawhan M R Mogarekar Rajesh K Jambhulkar Sanjay M Chawhan |
| author_sort | Seema S Chawhan |
| collection | DOAJ |
| description | Background: Paraoxonase2 (PON2) is an intracellular protein and widely present in many tissues such as endothelial cell and heart. It plays a major role in prevention of low-density lipoprotein (LDL) oxidation and reverses mildly oxidized LDL. PON2 plays an important role in antioxidation, antiatherosclerotic, and anti- inflammatory function. The oxidative stress leading to ischemic cell death involves the formation of reactive oxygen species/reactive nitrogen species through multiple injury mechanism.
Aim: The aim of our study was to assess and correlate monocytic PON2 activity, lipid profile, and total oxidative stress in patients with ischemic stroke.
Materials and Methods: The study population was included 50 ischemic stroke patients as cases and 50 healthy controls. The monocytic PON2 was measured spectrophotometrically using dihydrocoumarin as substrate. Serum lipid profile was estimated by established biochemical methods.
Results:The monocytic PON2 activity showed significant decrease in case (P < 0.001) while total oxidative stress showed significantly increases in cases (P < 0.001). Serum total cholesterol is significantly increases in cases (P value < 0.001) and serum HDL showed significant decrease in case (P < 0.005). Monocytic PON2 activity is significantly correlated with total oxidative stress (TOS) P =0.000). Multivariate logistic regression analysis used for prediction of risk of ischemic stroke contributed by various risk factor. The basic Model I shows significant P value and Naglekerke R2 value is 0.479. When PON2 activity is added to the basic model in the Model II, Naglekerke R2 value changes from 0.479 to 0.522, it also shows independent association of PON2 activity towards ischemic stroke. In linear regression analysis, significant positive correlation observed between PON2 and Ischemic stroke. PON2 lactonase activity was negatively correlated with total oxidative stress.
Conclusion: Our study strongly suggests that the estimation monocytic PON2 and total oxidative stress give valuable information for prediction of risk of ischemic stroke due to cerebrovascular thromboembolism and may consider as risk factors. |
| first_indexed | 2024-12-12T04:01:30Z |
| format | Article |
| id | doaj.art-a8acc586690f4383aebaed7dd7ac2b25 |
| institution | Directory Open Access Journal |
| issn | 2394-9481 2394-949X |
| language | English |
| last_indexed | 2024-12-12T04:01:30Z |
| publishDate | 2020-10-01 |
| publisher | ADICHUNCHANAGIRI INSTITUTE OF MEDICAL SCIENCES |
| record_format | Article |
| series | Journal of Medical Sciences and Health |
| spelling | doaj.art-a8acc586690f4383aebaed7dd7ac2b252022-12-22T00:39:02ZengADICHUNCHANAGIRI INSTITUTE OF MEDICAL SCIENCESJournal of Medical Sciences and Health2394-94812394-949X2020-10-0162813https://doi.org/10.46347/jmsh.2020.v06i02.002The Study of Paraoxonase 2, Lipid Profile, and Total Oxidative Stress in Ischemic StrokeSeema S Chawhan0M R Mogarekar1Rajesh K Jambhulkar2Sanjay M Chawhan3Assistant Professor, Department of Biochemistry, Government Medical College, Nagpur, Maharashtra, IndiaProfessor and Head, Department of Biochemistry, Swami Ramanand Teerth Rural Medical College, Ambajogai, Maharashtra, India,Assistant Professor, Department of Biochemistry, Government Medical College, Nagpur, Maharashtra, India,Associate Professor, Department of Pathology, Government Medical College, Gondia, Maharashtra, IndiaBackground: Paraoxonase2 (PON2) is an intracellular protein and widely present in many tissues such as endothelial cell and heart. It plays a major role in prevention of low-density lipoprotein (LDL) oxidation and reverses mildly oxidized LDL. PON2 plays an important role in antioxidation, antiatherosclerotic, and anti- inflammatory function. The oxidative stress leading to ischemic cell death involves the formation of reactive oxygen species/reactive nitrogen species through multiple injury mechanism. Aim: The aim of our study was to assess and correlate monocytic PON2 activity, lipid profile, and total oxidative stress in patients with ischemic stroke. Materials and Methods: The study population was included 50 ischemic stroke patients as cases and 50 healthy controls. The monocytic PON2 was measured spectrophotometrically using dihydrocoumarin as substrate. Serum lipid profile was estimated by established biochemical methods. Results:The monocytic PON2 activity showed significant decrease in case (P < 0.001) while total oxidative stress showed significantly increases in cases (P < 0.001). Serum total cholesterol is significantly increases in cases (P value < 0.001) and serum HDL showed significant decrease in case (P < 0.005). Monocytic PON2 activity is significantly correlated with total oxidative stress (TOS) P =0.000). Multivariate logistic regression analysis used for prediction of risk of ischemic stroke contributed by various risk factor. The basic Model I shows significant P value and Naglekerke R2 value is 0.479. When PON2 activity is added to the basic model in the Model II, Naglekerke R2 value changes from 0.479 to 0.522, it also shows independent association of PON2 activity towards ischemic stroke. In linear regression analysis, significant positive correlation observed between PON2 and Ischemic stroke. PON2 lactonase activity was negatively correlated with total oxidative stress. Conclusion: Our study strongly suggests that the estimation monocytic PON2 and total oxidative stress give valuable information for prediction of risk of ischemic stroke due to cerebrovascular thromboembolism and may consider as risk factors.https://jmsh.ac.in/index.php?option=com_k2&view=item&id=144:the-study-of-paraoxonase-2-lipid-profile-and-total-oxidative-stress-in-ischemic-stroke&Itemid=1paraoxonase 2total oxidative stresslipid profileischemic stroke. |
| spellingShingle | Seema S Chawhan M R Mogarekar Rajesh K Jambhulkar Sanjay M Chawhan The Study of Paraoxonase 2, Lipid Profile, and Total Oxidative Stress in Ischemic Stroke Journal of Medical Sciences and Health paraoxonase 2 total oxidative stress lipid profile ischemic stroke. |
| title | The Study of Paraoxonase 2, Lipid Profile, and Total Oxidative Stress in Ischemic Stroke |
| title_full | The Study of Paraoxonase 2, Lipid Profile, and Total Oxidative Stress in Ischemic Stroke |
| title_fullStr | The Study of Paraoxonase 2, Lipid Profile, and Total Oxidative Stress in Ischemic Stroke |
| title_full_unstemmed | The Study of Paraoxonase 2, Lipid Profile, and Total Oxidative Stress in Ischemic Stroke |
| title_short | The Study of Paraoxonase 2, Lipid Profile, and Total Oxidative Stress in Ischemic Stroke |
| title_sort | study of paraoxonase 2 lipid profile and total oxidative stress in ischemic stroke |
| topic | paraoxonase 2 total oxidative stress lipid profile ischemic stroke. |
| url | https://jmsh.ac.in/index.php?option=com_k2&view=item&id=144:the-study-of-paraoxonase-2-lipid-profile-and-total-oxidative-stress-in-ischemic-stroke&Itemid=1 |
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