Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics
Nanoparticles (NPs) and antibiotic resistant genes (ARGs), as emerging environmental contaminants, have been reported to be accumulated in the soil environment. The use of NPs have raised increasing concerns about their environmental impacts, but the combined effect of NPs and antibiotics on ARGs re...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2020-01-01
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| Series: | Environmental Research Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/1748-9326/ab7131 |
| _version_ | 1797747611521777664 |
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| author | Xiujuan Zhang Junjian Li Dale Li Hong Zhang Hangwei Hu |
| author_facet | Xiujuan Zhang Junjian Li Dale Li Hong Zhang Hangwei Hu |
| author_sort | Xiujuan Zhang |
| collection | DOAJ |
| description | Nanoparticles (NPs) and antibiotic resistant genes (ARGs), as emerging environmental contaminants, have been reported to be accumulated in the soil environment. The use of NPs have raised increasing concerns about their environmental impacts, but the combined effect of NPs and antibiotics on ARGs remains less understood. Here, we established laboratory microcosms to explore the impacts of different concentrations of SiO _2 NPs on β -lactam and sulfonamide resistance genes in soils amended with β -lactam or sulfonamide. Illumina sequencing and quantitative PCR revealed that the addition of NPs increased the bacterial community diversity but had no significant effects on the bacterial abundance. Moreover, NPs and sulfonamide jointly increased the abundances of sulfonamide resistance genes, while the exposure of NPs and β -lactam decreased β -lactam resistance genes. The detected ARGs were associated closely with two mobile genetic elements (MGEs, the tnp A and int I1 genes), indicating that MGEs may contribute to the dissemination of ARGs. Correlation analysis indicated the shifts in potential bacterial hosts and the frequency of horizontal gene transfer were important factors explaining the patterns of ARGs. Furthermore, structural equation models indicated that NPs exposure decreased the abundances of β -lactam resistance genes by driving changes in bacterial community and MGEs, whereas the increased abundances of sulfonamide resistance genes were mainly associated with the bacterial community, diversity and MGEs mediated by NPs and antibiotics. These results suggested that the combined effects of NPs and antibiotics on soil bacterial resistance were different due to the types of antibiotics. |
| first_indexed | 2024-03-12T15:53:05Z |
| format | Article |
| id | doaj.art-a8acd4c308fa4027b76ea0e8951ecdb3 |
| institution | Directory Open Access Journal |
| issn | 1748-9326 |
| language | English |
| last_indexed | 2024-03-12T15:53:05Z |
| publishDate | 2020-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Environmental Research Letters |
| spelling | doaj.art-a8acd4c308fa4027b76ea0e8951ecdb32023-08-09T15:03:29ZengIOP PublishingEnvironmental Research Letters1748-93262020-01-0115303400110.1088/1748-9326/ab7131Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibioticsXiujuan Zhang0Junjian Li1Dale Li2Hong Zhang3Hangwei Hu4Institute of Loess Plateau, Shanxi University , Taiyuan, Shanxi, 030006, People’s Republic of ChinaInstitute of Loess Plateau, Shanxi University , Taiyuan, Shanxi, 030006, People’s Republic of ChinaInstitute of Loess Plateau, Shanxi University , Taiyuan, Shanxi, 030006, People’s Republic of ChinaSchool of Environment and Resources, Shanxi University , Taiyuan 030006, People’s Republic of ChinaSchool of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne , Victoria, 3010, AustraliaNanoparticles (NPs) and antibiotic resistant genes (ARGs), as emerging environmental contaminants, have been reported to be accumulated in the soil environment. The use of NPs have raised increasing concerns about their environmental impacts, but the combined effect of NPs and antibiotics on ARGs remains less understood. Here, we established laboratory microcosms to explore the impacts of different concentrations of SiO _2 NPs on β -lactam and sulfonamide resistance genes in soils amended with β -lactam or sulfonamide. Illumina sequencing and quantitative PCR revealed that the addition of NPs increased the bacterial community diversity but had no significant effects on the bacterial abundance. Moreover, NPs and sulfonamide jointly increased the abundances of sulfonamide resistance genes, while the exposure of NPs and β -lactam decreased β -lactam resistance genes. The detected ARGs were associated closely with two mobile genetic elements (MGEs, the tnp A and int I1 genes), indicating that MGEs may contribute to the dissemination of ARGs. Correlation analysis indicated the shifts in potential bacterial hosts and the frequency of horizontal gene transfer were important factors explaining the patterns of ARGs. Furthermore, structural equation models indicated that NPs exposure decreased the abundances of β -lactam resistance genes by driving changes in bacterial community and MGEs, whereas the increased abundances of sulfonamide resistance genes were mainly associated with the bacterial community, diversity and MGEs mediated by NPs and antibiotics. These results suggested that the combined effects of NPs and antibiotics on soil bacterial resistance were different due to the types of antibiotics.https://doi.org/10.1088/1748-9326/ab7131SiO2 nanoparticlesantibiotic resistance genesmobile genetic elementbacterial composition |
| spellingShingle | Xiujuan Zhang Junjian Li Dale Li Hong Zhang Hangwei Hu Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics Environmental Research Letters SiO2 nanoparticles antibiotic resistance genes mobile genetic element bacterial composition |
| title | Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics |
| title_full | Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics |
| title_fullStr | Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics |
| title_full_unstemmed | Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics |
| title_short | Silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β-lactam resistance genes in soils amended with antibiotics |
| title_sort | silicon dioxide nanoparticles have contrasting effects on the temporal dynamics of sulfonamide and β lactam resistance genes in soils amended with antibiotics |
| topic | SiO2 nanoparticles antibiotic resistance genes mobile genetic element bacterial composition |
| url | https://doi.org/10.1088/1748-9326/ab7131 |
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