Asymmetric neurogenic commitment of retinal progenitors involves Notch through the endocytic pathway
During brain development, progenitor cells need to balanceproliferation and differentiation in order to generate different neurons in the correct numbers and proportions. Currently, the patterns of multipotent progenitor divisions that lead to neurogenic entry and the factors that regulate them are...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-11-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/60462 |
| _version_ | 1811200251516682240 |
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| author | Elisa Nerli Mauricio Rocha-Martins Caren Norden |
| author_facet | Elisa Nerli Mauricio Rocha-Martins Caren Norden |
| author_sort | Elisa Nerli |
| collection | DOAJ |
| description | During brain development, progenitor cells need to balanceproliferation and differentiation in order to generate different neurons in the correct numbers and proportions. Currently, the patterns of multipotent progenitor divisions that lead to neurogenic entry and the factors that regulate them are not fully understood. We here use the zebrafish retina to address this gap, exploiting its suitability for quantitative live-imaging. We show that early neurogenic progenitors arise from asymmetric divisions. Notch regulates this asymmetry, as when inhibited, symmetric divisions producing two neurogenic progenitors occur. Surprisingly however, Notch does not act through an apicobasal activity gradient as previously suggested, but through asymmetric inheritance of Sara-positive endosomes. Further, the resulting neurogenic progenitors show cell biological features different from multipotent progenitors, raising the possibility that an intermediate progenitor state exists in the retina. Our study thus reveals new insights into the regulation of proliferative and differentiative events during central nervous system development. |
| first_indexed | 2024-04-12T02:01:34Z |
| format | Article |
| id | doaj.art-a8aeb3f98235463d9dbed438a01236cf |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:01:34Z |
| publishDate | 2020-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a8aeb3f98235463d9dbed438a01236cf2022-12-22T03:52:40ZengeLife Sciences Publications LtdeLife2050-084X2020-11-01910.7554/eLife.60462Asymmetric neurogenic commitment of retinal progenitors involves Notch through the endocytic pathwayElisa Nerli0https://orcid.org/0000-0003-4204-9702Mauricio Rocha-Martins1Caren Norden2https://orcid.org/0000-0001-8835-1451Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; Instituto Gulbenkian de Ciência, Oeiras, PortugalMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; Instituto Gulbenkian de Ciência, Oeiras, PortugalMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; Instituto Gulbenkian de Ciência, Oeiras, PortugalDuring brain development, progenitor cells need to balanceproliferation and differentiation in order to generate different neurons in the correct numbers and proportions. Currently, the patterns of multipotent progenitor divisions that lead to neurogenic entry and the factors that regulate them are not fully understood. We here use the zebrafish retina to address this gap, exploiting its suitability for quantitative live-imaging. We show that early neurogenic progenitors arise from asymmetric divisions. Notch regulates this asymmetry, as when inhibited, symmetric divisions producing two neurogenic progenitors occur. Surprisingly however, Notch does not act through an apicobasal activity gradient as previously suggested, but through asymmetric inheritance of Sara-positive endosomes. Further, the resulting neurogenic progenitors show cell biological features different from multipotent progenitors, raising the possibility that an intermediate progenitor state exists in the retina. Our study thus reveals new insights into the regulation of proliferative and differentiative events during central nervous system development.https://elifesciences.org/articles/60462quantitative live-imagingretinal developmentfate asymmetryNotchSara endosomes |
| spellingShingle | Elisa Nerli Mauricio Rocha-Martins Caren Norden Asymmetric neurogenic commitment of retinal progenitors involves Notch through the endocytic pathway eLife quantitative live-imaging retinal development fate asymmetry Notch Sara endosomes |
| title | Asymmetric neurogenic commitment of retinal progenitors involves Notch through the endocytic pathway |
| title_full | Asymmetric neurogenic commitment of retinal progenitors involves Notch through the endocytic pathway |
| title_fullStr | Asymmetric neurogenic commitment of retinal progenitors involves Notch through the endocytic pathway |
| title_full_unstemmed | Asymmetric neurogenic commitment of retinal progenitors involves Notch through the endocytic pathway |
| title_short | Asymmetric neurogenic commitment of retinal progenitors involves Notch through the endocytic pathway |
| title_sort | asymmetric neurogenic commitment of retinal progenitors involves notch through the endocytic pathway |
| topic | quantitative live-imaging retinal development fate asymmetry Notch Sara endosomes |
| url | https://elifesciences.org/articles/60462 |
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