Overexpression of HOTAIR in Tumor Tissues of Patients with Colon Cancer Correlates with Tumor Metastasis and Differentiation
<strong>Background:</strong> Aberrant expression level of Hox transcript antisense intergenic RNA (HOTAIR) has been associated with the etiopathogenesis of numerous cancers. Studies on epidemiological data have demonstrated that the risk of susceptibility to colon cancer varies among dif...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2020-10-01
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| Series: | Middle East Journal of Cancer |
| Subjects: | |
| Online Access: | https://mejc.sums.ac.ir/article_46775_74cff15d8353e998ead29d8a342b4e25.pdf |
| _version_ | 1818085148025946112 |
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| author | Shadi Pashapour Dariush Shanehbandi Soghra Bornehdeli Venus Zafari Haniye Mohammad Reza Khani Shahriyar Hashemzadeh Touraj Asvadi Kermani |
| author_facet | Shadi Pashapour Dariush Shanehbandi Soghra Bornehdeli Venus Zafari Haniye Mohammad Reza Khani Shahriyar Hashemzadeh Touraj Asvadi Kermani |
| author_sort | Shadi Pashapour |
| collection | DOAJ |
| description | <strong>Background:</strong> Aberrant expression level of Hox transcript antisense intergenic RNA (HOTAIR) has been associated with the etiopathogenesis of numerous cancers. Studies on epidemiological data have demonstrated that the risk of susceptibility to colon cancer varies among different populations due to several reasons. In this study, we aimed to assess the expression level of HOTAIR in tumoral tissues of patients with colon cancer and compare it with normal marginal tissues.
<strong>Methods:</strong> In this case-control study, we recruited a total of 50 patients with colon cancer and collected tumoral and matched marginal tumor free tissues during surgery. Afterwards, we isolated the total RNA from each sample, synthesized cDNA, and performed quantitative analysis by Real-time PCR using the SYBR Green PCR Master Mix in order to measure the transcript level of HOTAIR in samples.
<strong>Results:</strong> The expression level of HOTAIR was upregulated in tumor tissues compared with normal tumor-free marginal tissues belonging to colon cancer patients (P= 0.0023). Moreover, the expression level of HOTAIR and the clinicopathological specifications of the patients had statistically significant correlations.
<strong>Conclusions:</strong> HOTAIR may play a role in the development of colon cancer and have the potential for application as a biomarker for colon cancer prognosis. |
| first_indexed | 2024-12-10T20:05:10Z |
| format | Article |
| id | doaj.art-a8aec4935ef84af4801b74802ee3a85b |
| institution | Directory Open Access Journal |
| issn | 2008-6709 2008-6687 |
| language | English |
| last_indexed | 2024-12-10T20:05:10Z |
| publishDate | 2020-10-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Middle East Journal of Cancer |
| spelling | doaj.art-a8aec4935ef84af4801b74802ee3a85b2022-12-22T01:35:25ZengShiraz University of Medical SciencesMiddle East Journal of Cancer2008-67092008-66872020-10-0111441041410.30476/mejc.2020.81442.101046775Overexpression of HOTAIR in Tumor Tissues of Patients with Colon Cancer Correlates with Tumor Metastasis and DifferentiationShadi Pashapour0Dariush Shanehbandi1Soghra Bornehdeli2Venus Zafari3Haniye Mohammad Reza Khani4Shahriyar Hashemzadeh5Touraj Asvadi Kermani6Department of Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Immunology Research Center, University Tabriz University of Medical Sciences, Tabriz, IranDepartment of Immunology Research Center, University Tabriz University of Medical Sciences, Tabriz, IranDepartment of Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Immunology Research Center, University Tabriz University of Medical Sciences, Tabriz, IranDepartment of Immunology Research Center, University Tabriz University of Medical Sciences, Tabriz, IranDepartment of Surgery, Imam Reza Hospital, University Tabriz University of Medical Sciences, Tabriz, Iran<strong>Background:</strong> Aberrant expression level of Hox transcript antisense intergenic RNA (HOTAIR) has been associated with the etiopathogenesis of numerous cancers. Studies on epidemiological data have demonstrated that the risk of susceptibility to colon cancer varies among different populations due to several reasons. In this study, we aimed to assess the expression level of HOTAIR in tumoral tissues of patients with colon cancer and compare it with normal marginal tissues. <strong>Methods:</strong> In this case-control study, we recruited a total of 50 patients with colon cancer and collected tumoral and matched marginal tumor free tissues during surgery. Afterwards, we isolated the total RNA from each sample, synthesized cDNA, and performed quantitative analysis by Real-time PCR using the SYBR Green PCR Master Mix in order to measure the transcript level of HOTAIR in samples. <strong>Results:</strong> The expression level of HOTAIR was upregulated in tumor tissues compared with normal tumor-free marginal tissues belonging to colon cancer patients (P= 0.0023). Moreover, the expression level of HOTAIR and the clinicopathological specifications of the patients had statistically significant correlations. <strong>Conclusions:</strong> HOTAIR may play a role in the development of colon cancer and have the potential for application as a biomarker for colon cancer prognosis.https://mejc.sums.ac.ir/article_46775_74cff15d8353e998ead29d8a342b4e25.pdfcolorectal cancerhotairtranscriptioncancer biomarker |
| spellingShingle | Shadi Pashapour Dariush Shanehbandi Soghra Bornehdeli Venus Zafari Haniye Mohammad Reza Khani Shahriyar Hashemzadeh Touraj Asvadi Kermani Overexpression of HOTAIR in Tumor Tissues of Patients with Colon Cancer Correlates with Tumor Metastasis and Differentiation Middle East Journal of Cancer colorectal cancer hotair transcription cancer biomarker |
| title | Overexpression of HOTAIR in Tumor Tissues of Patients with Colon Cancer Correlates with Tumor Metastasis and Differentiation |
| title_full | Overexpression of HOTAIR in Tumor Tissues of Patients with Colon Cancer Correlates with Tumor Metastasis and Differentiation |
| title_fullStr | Overexpression of HOTAIR in Tumor Tissues of Patients with Colon Cancer Correlates with Tumor Metastasis and Differentiation |
| title_full_unstemmed | Overexpression of HOTAIR in Tumor Tissues of Patients with Colon Cancer Correlates with Tumor Metastasis and Differentiation |
| title_short | Overexpression of HOTAIR in Tumor Tissues of Patients with Colon Cancer Correlates with Tumor Metastasis and Differentiation |
| title_sort | overexpression of hotair in tumor tissues of patients with colon cancer correlates with tumor metastasis and differentiation |
| topic | colorectal cancer hotair transcription cancer biomarker |
| url | https://mejc.sums.ac.ir/article_46775_74cff15d8353e998ead29d8a342b4e25.pdf |
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