Transcriptional Plasticity and Cell Wall Characterization in High-Methanol-Producing Transgenic Tobacco Plants
Volatile organic compounds emanating from plant surfaces serve as a sustainable natural solution to combat biotic stresses in plants. Leaf methanol is the simplest and second major volatile organic compound after isoprene emitted through the leaf surface. Methanol has been neglected as a by-product...
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MDPI AG
2023-02-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/13/3/521 |
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| author | Sameer Dixit Krishnappa Chandrashekar Santosh Kumar Upadhyay Praveen Chandra Verma |
| author_facet | Sameer Dixit Krishnappa Chandrashekar Santosh Kumar Upadhyay Praveen Chandra Verma |
| author_sort | Sameer Dixit |
| collection | DOAJ |
| description | Volatile organic compounds emanating from plant surfaces serve as a sustainable natural solution to combat biotic stresses in plants. Leaf methanol is the simplest and second major volatile organic compound after isoprene emitted through the leaf surface. Methanol has been neglected as a by-product of other secondary metabolites for a long time, but recent studies have suggested its importance in development and stress responses. In our previous findings, we had revealed that transgenic plants over-expressing PME, enhanced methanol production providing resistance against a broad spectrum of insects. In the current study, we extended our previous work to provide new insights by performing differential transcriptomics of high-methanol-producing insect-resistant transgenic lines. We found that 2262 genes were differentially expressed in the transgenics plants, including transcription factors, cell wall modulating, phytohormones signaling and development-related genes. Our results demonstrated that the expression levels of transcription factors associated with development and biotic stress were altered in the transgenic lines. In addition, phytohormones ABA and gibberellin signalling genes were upregulated, whereas ethylene and auxin signalling genes were downregulated. Moreover, biochemical characteristics of cell walls in both transgenic tobacco plants were comparable to the control plants. |
| first_indexed | 2024-03-11T07:04:52Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-11T07:04:52Z |
| publishDate | 2023-02-01 |
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| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-a8b53cf8cf48431193b4e1eabb3157ed2023-11-17T08:59:37ZengMDPI AGAgriculture2077-04722023-02-0113352110.3390/agriculture13030521Transcriptional Plasticity and Cell Wall Characterization in High-Methanol-Producing Transgenic Tobacco PlantsSameer Dixit0Krishnappa Chandrashekar1Santosh Kumar Upadhyay2Praveen Chandra Verma3CSIR-National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, UP, IndiaCSIR-National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, UP, IndiaDepartment of Botany, Panjab University, Sector 14, Chandigarh 160014, UT, IndiaCSIR-National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, UP, IndiaVolatile organic compounds emanating from plant surfaces serve as a sustainable natural solution to combat biotic stresses in plants. Leaf methanol is the simplest and second major volatile organic compound after isoprene emitted through the leaf surface. Methanol has been neglected as a by-product of other secondary metabolites for a long time, but recent studies have suggested its importance in development and stress responses. In our previous findings, we had revealed that transgenic plants over-expressing PME, enhanced methanol production providing resistance against a broad spectrum of insects. In the current study, we extended our previous work to provide new insights by performing differential transcriptomics of high-methanol-producing insect-resistant transgenic lines. We found that 2262 genes were differentially expressed in the transgenics plants, including transcription factors, cell wall modulating, phytohormones signaling and development-related genes. Our results demonstrated that the expression levels of transcription factors associated with development and biotic stress were altered in the transgenic lines. In addition, phytohormones ABA and gibberellin signalling genes were upregulated, whereas ethylene and auxin signalling genes were downregulated. Moreover, biochemical characteristics of cell walls in both transgenic tobacco plants were comparable to the control plants.https://www.mdpi.com/2077-0472/13/3/521methanolinsect resistancemicroarrayconfocal microscopypectin methylesterasecell wall |
| spellingShingle | Sameer Dixit Krishnappa Chandrashekar Santosh Kumar Upadhyay Praveen Chandra Verma Transcriptional Plasticity and Cell Wall Characterization in High-Methanol-Producing Transgenic Tobacco Plants Agriculture methanol insect resistance microarray confocal microscopy pectin methylesterase cell wall |
| title | Transcriptional Plasticity and Cell Wall Characterization in High-Methanol-Producing Transgenic Tobacco Plants |
| title_full | Transcriptional Plasticity and Cell Wall Characterization in High-Methanol-Producing Transgenic Tobacco Plants |
| title_fullStr | Transcriptional Plasticity and Cell Wall Characterization in High-Methanol-Producing Transgenic Tobacco Plants |
| title_full_unstemmed | Transcriptional Plasticity and Cell Wall Characterization in High-Methanol-Producing Transgenic Tobacco Plants |
| title_short | Transcriptional Plasticity and Cell Wall Characterization in High-Methanol-Producing Transgenic Tobacco Plants |
| title_sort | transcriptional plasticity and cell wall characterization in high methanol producing transgenic tobacco plants |
| topic | methanol insect resistance microarray confocal microscopy pectin methylesterase cell wall |
| url | https://www.mdpi.com/2077-0472/13/3/521 |
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