Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap.
TGIFLX, a Homoproteins cluster member located on the X chromosome, has a critical role in male reproduction and prostate development. Previous studies have shown the erratic expression of TGIFLX gene in a large proportion of prostate tumors. However TGIFLX function in prostate development remains un...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2013-11-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/4356 |
| _version_ | 1829521176565645312 |
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| author | Zahra Rashvand Mansour Heidari Reza Raoofian Mohammad Hossein Modarresi Reza Shirkoohi |
| author_facet | Zahra Rashvand Mansour Heidari Reza Raoofian Mohammad Hossein Modarresi Reza Shirkoohi |
| author_sort | Zahra Rashvand |
| collection | DOAJ |
| description | TGIFLX, a Homoproteins cluster member located on the X chromosome, has a critical role in male reproduction and prostate development. Previous studies have shown the erratic expression of TGIFLX gene in a large proportion of prostate tumors. However TGIFLX function in prostate development remains unknown. The purpose of this study was to evaluate the consequences of TGIFLX expression on prostate cancer cell lines (LNCaP).Inducible Tet-On gene expression system was used with a regulatory capability by doxycycline induction. In this system, stable LNCaP cells with TGIFLX tet-on plasmid were able to induce TGIFLX expression by doxycycline treatment. TGIFLX gene expression was confirmed by RT-PCR.Induction of gene expression caused cell proliferation decrement and apoptosis increment in LNCaP TGIFLX cells compared with control cells (P |
| first_indexed | 2024-12-16T15:10:07Z |
| format | Article |
| id | doaj.art-a8b791f7a30643d1afe4afc46326a020 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-16T15:10:07Z |
| publishDate | 2013-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-a8b791f7a30643d1afe4afc46326a0202022-12-21T22:26:59ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932013-11-0142114211Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap.Zahra Rashvand0Mansour Heidari1Reza Raoofian2Mohammad Hossein Modarresi3Reza Shirkoohi41. Dept. of Medical Genetics, Tehran University of Medical Sciences (TUMS) , Tehran, Iran.1. Dept. of Medical Genetics, Tehran University of Medical Sciences (TUMS) , Tehran, Iran ; 2. Stem Cell Preparation Unit, Farabi Eye Hospital, Tehran University of Medical Sciences , Tehran, Iran.1. Dept. of Medical Genetics, Tehran University of Medical Sciences (TUMS) , Tehran, Iran.1. Dept. of Medical Genetics, Tehran University of Medical Sciences (TUMS) , Tehran, Iran.3. Group of Genetics, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences (TUMS) , Tehran, Iran.TGIFLX, a Homoproteins cluster member located on the X chromosome, has a critical role in male reproduction and prostate development. Previous studies have shown the erratic expression of TGIFLX gene in a large proportion of prostate tumors. However TGIFLX function in prostate development remains unknown. The purpose of this study was to evaluate the consequences of TGIFLX expression on prostate cancer cell lines (LNCaP).Inducible Tet-On gene expression system was used with a regulatory capability by doxycycline induction. In this system, stable LNCaP cells with TGIFLX tet-on plasmid were able to induce TGIFLX expression by doxycycline treatment. TGIFLX gene expression was confirmed by RT-PCR.Induction of gene expression caused cell proliferation decrement and apoptosis increment in LNCaP TGIFLX cells compared with control cells (Phttps://ijph.tums.ac.ir/index.php/ijph/article/view/4356LNCaPProstate cancerStable cell lineTGIFLX geneTet-On vector |
| spellingShingle | Zahra Rashvand Mansour Heidari Reza Raoofian Mohammad Hossein Modarresi Reza Shirkoohi Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap. Iranian Journal of Public Health LNCaP Prostate cancer Stable cell line TGIFLX gene Tet-On vector |
| title | Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap. |
| title_full | Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap. |
| title_fullStr | Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap. |
| title_full_unstemmed | Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap. |
| title_short | Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap. |
| title_sort | induction of apoptosis and growth suppression by homeobox gene tgiflx in prostate cancer cell line lncap |
| topic | LNCaP Prostate cancer Stable cell line TGIFLX gene Tet-On vector |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/4356 |
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