The ex planta signal activity of a Medicago ribosomal uL2 protein suggests a moonlighting role in controlling secondary rhizobial infection
We recently described a regulatory loop, which we termed autoregulation of infection (AOI), by which Sinorhizobium meliloti, a Medicago endosymbiont, downregulates the root susceptibility to secondary infection events via ethylene. AOI is initially triggered by so-far unidentified Medicago nodule si...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529298/?tool=EBI |
| _version_ | 1818538706613567488 |
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| author | Fernando Sorroche Violette Morales Saïda Mouffok Carole Pichereaux A. Marie Garnerone Lan Zou Badrish Soni Marie-Anne Carpéné Audrey Gargaros Fabienne Maillet Odile Burlet-Schiltz Verena Poinsot Patrice Polard Clare Gough Jacques Batut Francisco Martinez-Abarca |
| author_facet | Fernando Sorroche Violette Morales Saïda Mouffok Carole Pichereaux A. Marie Garnerone Lan Zou Badrish Soni Marie-Anne Carpéné Audrey Gargaros Fabienne Maillet Odile Burlet-Schiltz Verena Poinsot Patrice Polard Clare Gough Jacques Batut Francisco Martinez-Abarca |
| author_sort | Fernando Sorroche |
| collection | DOAJ |
| description | We recently described a regulatory loop, which we termed autoregulation of infection (AOI), by which Sinorhizobium meliloti, a Medicago endosymbiont, downregulates the root susceptibility to secondary infection events via ethylene. AOI is initially triggered by so-far unidentified Medicago nodule signals named signal 1 and signal 1’ whose transduction in bacteroids requires the S. meliloti outer-membrane-associated NsrA receptor protein and the cognate inner-membrane-associated adenylate cyclases, CyaK and CyaD1/D2, respectively. Here, we report on advances in signal 1 identification. Signal 1 activity is widespread as we robustly detected it in Medicago nodule extracts as well as in yeast and bacteria cell extracts. Biochemical analyses indicated a peptidic nature for signal 1 and, together with proteomic analyses, a universally conserved Medicago ribosomal protein of the uL2 family was identified as a candidate signal 1. Specifically, MtRPuL2A (MtrunA17Chr7g0247311) displays a strong signal activity that requires S. meliloti NsrA and CyaK, as endogenous signal 1. We have shown that MtRPuL2A is active in signaling only in a non-ribosomal form. A Medicago truncatula mutant in the major symbiotic transcriptional regulator MtNF-YA1 lacked most signal 1 activity, suggesting that signal 1 is under developmental control. Altogether, our results point to the MtRPuL2A ribosomal protein as the candidate for signal 1. Based on the Mtnf-ya1 mutant, we suggest a link between root infectiveness and nodule development. We discuss our findings in the context of ribosomal protein moonlighting. |
| first_indexed | 2024-12-11T21:32:25Z |
| format | Article |
| id | doaj.art-a8bdde53bebf4a89bdd4fbb9f06371fa |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T21:32:25Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a8bdde53bebf4a89bdd4fbb9f06371fa2022-12-22T00:50:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011510The ex planta signal activity of a Medicago ribosomal uL2 protein suggests a moonlighting role in controlling secondary rhizobial infectionFernando SorrocheViolette MoralesSaïda MouffokCarole PichereauxA. Marie GarneroneLan ZouBadrish SoniMarie-Anne CarpénéAudrey GargarosFabienne MailletOdile Burlet-SchiltzVerena PoinsotPatrice PolardClare GoughJacques BatutFrancisco Martinez-AbarcaWe recently described a regulatory loop, which we termed autoregulation of infection (AOI), by which Sinorhizobium meliloti, a Medicago endosymbiont, downregulates the root susceptibility to secondary infection events via ethylene. AOI is initially triggered by so-far unidentified Medicago nodule signals named signal 1 and signal 1’ whose transduction in bacteroids requires the S. meliloti outer-membrane-associated NsrA receptor protein and the cognate inner-membrane-associated adenylate cyclases, CyaK and CyaD1/D2, respectively. Here, we report on advances in signal 1 identification. Signal 1 activity is widespread as we robustly detected it in Medicago nodule extracts as well as in yeast and bacteria cell extracts. Biochemical analyses indicated a peptidic nature for signal 1 and, together with proteomic analyses, a universally conserved Medicago ribosomal protein of the uL2 family was identified as a candidate signal 1. Specifically, MtRPuL2A (MtrunA17Chr7g0247311) displays a strong signal activity that requires S. meliloti NsrA and CyaK, as endogenous signal 1. We have shown that MtRPuL2A is active in signaling only in a non-ribosomal form. A Medicago truncatula mutant in the major symbiotic transcriptional regulator MtNF-YA1 lacked most signal 1 activity, suggesting that signal 1 is under developmental control. Altogether, our results point to the MtRPuL2A ribosomal protein as the candidate for signal 1. Based on the Mtnf-ya1 mutant, we suggest a link between root infectiveness and nodule development. We discuss our findings in the context of ribosomal protein moonlighting.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529298/?tool=EBI |
| spellingShingle | Fernando Sorroche Violette Morales Saïda Mouffok Carole Pichereaux A. Marie Garnerone Lan Zou Badrish Soni Marie-Anne Carpéné Audrey Gargaros Fabienne Maillet Odile Burlet-Schiltz Verena Poinsot Patrice Polard Clare Gough Jacques Batut Francisco Martinez-Abarca The ex planta signal activity of a Medicago ribosomal uL2 protein suggests a moonlighting role in controlling secondary rhizobial infection PLoS ONE |
| title | The ex planta signal activity of a Medicago ribosomal uL2 protein suggests a moonlighting role in controlling secondary rhizobial infection |
| title_full | The ex planta signal activity of a Medicago ribosomal uL2 protein suggests a moonlighting role in controlling secondary rhizobial infection |
| title_fullStr | The ex planta signal activity of a Medicago ribosomal uL2 protein suggests a moonlighting role in controlling secondary rhizobial infection |
| title_full_unstemmed | The ex planta signal activity of a Medicago ribosomal uL2 protein suggests a moonlighting role in controlling secondary rhizobial infection |
| title_short | The ex planta signal activity of a Medicago ribosomal uL2 protein suggests a moonlighting role in controlling secondary rhizobial infection |
| title_sort | ex planta signal activity of a medicago ribosomal ul2 protein suggests a moonlighting role in controlling secondary rhizobial infection |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529298/?tool=EBI |
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