Widespread occurrence of expressed fungal secretory peroxidases in forest soils.
Fungal secretory peroxidases mediate fundamental ecological functions in the conversion and degradation of plant biomass. Many of these enzymes have strong oxidizing activities towards aromatic compounds and are involved in the degradation of plant cell wall (lignin) and humus. They comprise three m...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0095557&type=printable |
| _version_ | 1826587120760782848 |
|---|---|
| author | Harald Kellner Patricia Luis Marek J Pecyna Florian Barbi Danuta Kapturska Dirk Krüger Donald R Zak Roland Marmeisse Micheline Vandenbol Martin Hofrichter |
| author_facet | Harald Kellner Patricia Luis Marek J Pecyna Florian Barbi Danuta Kapturska Dirk Krüger Donald R Zak Roland Marmeisse Micheline Vandenbol Martin Hofrichter |
| author_sort | Harald Kellner |
| collection | DOAJ |
| description | Fungal secretory peroxidases mediate fundamental ecological functions in the conversion and degradation of plant biomass. Many of these enzymes have strong oxidizing activities towards aromatic compounds and are involved in the degradation of plant cell wall (lignin) and humus. They comprise three major groups: class II peroxidases (including lignin peroxidase, manganese peroxidase, versatile peroxidase and generic peroxidase), dye-decolorizing peroxidases, and heme-thiolate peroxidases (e.g. unspecific/aromatic peroxygenase, chloroperoxidase). Here, we have repeatedly observed a widespread expression of all major peroxidase groups in leaf and needle litter across a range of forest ecosystems (e.g. Fagus, Picea, Acer, Quercus, and Populus spp.), which are widespread in Europe and North America. Manganese peroxidases and unspecific peroxygenases were found expressed in all nine investigated forest sites, and dye-decolorizing peroxidases were observed in five of the nine sites, thereby indicating biological significance of these enzymes for fungal physiology and ecosystem processes. Transcripts of selected secretory peroxidase genes were also analyzed in pure cultures of several litter-decomposing species and other fungi. Using this information, we were able to match, in environmental litter samples, two manganese peroxidase sequences to Mycena galopus and Mycena epipterygia and one unspecific peroxygenase transcript to Mycena galopus, suggesting an important role of this litter- and coarse woody debris-dwelling genus in the disintegration and transformation of litter aromatics and organic matter formation. |
| first_indexed | 2024-12-19T19:36:29Z |
| format | Article |
| id | doaj.art-a8be3bc9aa68424591645bdc14d74c64 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T16:20:23Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a8be3bc9aa68424591645bdc14d74c642025-02-22T05:33:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9555710.1371/journal.pone.0095557Widespread occurrence of expressed fungal secretory peroxidases in forest soils.Harald KellnerPatricia LuisMarek J PecynaFlorian BarbiDanuta KapturskaDirk KrügerDonald R ZakRoland MarmeisseMicheline VandenbolMartin HofrichterFungal secretory peroxidases mediate fundamental ecological functions in the conversion and degradation of plant biomass. Many of these enzymes have strong oxidizing activities towards aromatic compounds and are involved in the degradation of plant cell wall (lignin) and humus. They comprise three major groups: class II peroxidases (including lignin peroxidase, manganese peroxidase, versatile peroxidase and generic peroxidase), dye-decolorizing peroxidases, and heme-thiolate peroxidases (e.g. unspecific/aromatic peroxygenase, chloroperoxidase). Here, we have repeatedly observed a widespread expression of all major peroxidase groups in leaf and needle litter across a range of forest ecosystems (e.g. Fagus, Picea, Acer, Quercus, and Populus spp.), which are widespread in Europe and North America. Manganese peroxidases and unspecific peroxygenases were found expressed in all nine investigated forest sites, and dye-decolorizing peroxidases were observed in five of the nine sites, thereby indicating biological significance of these enzymes for fungal physiology and ecosystem processes. Transcripts of selected secretory peroxidase genes were also analyzed in pure cultures of several litter-decomposing species and other fungi. Using this information, we were able to match, in environmental litter samples, two manganese peroxidase sequences to Mycena galopus and Mycena epipterygia and one unspecific peroxygenase transcript to Mycena galopus, suggesting an important role of this litter- and coarse woody debris-dwelling genus in the disintegration and transformation of litter aromatics and organic matter formation.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0095557&type=printable |
| spellingShingle | Harald Kellner Patricia Luis Marek J Pecyna Florian Barbi Danuta Kapturska Dirk Krüger Donald R Zak Roland Marmeisse Micheline Vandenbol Martin Hofrichter Widespread occurrence of expressed fungal secretory peroxidases in forest soils. PLoS ONE |
| title | Widespread occurrence of expressed fungal secretory peroxidases in forest soils. |
| title_full | Widespread occurrence of expressed fungal secretory peroxidases in forest soils. |
| title_fullStr | Widespread occurrence of expressed fungal secretory peroxidases in forest soils. |
| title_full_unstemmed | Widespread occurrence of expressed fungal secretory peroxidases in forest soils. |
| title_short | Widespread occurrence of expressed fungal secretory peroxidases in forest soils. |
| title_sort | widespread occurrence of expressed fungal secretory peroxidases in forest soils |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0095557&type=printable |
| work_keys_str_mv | AT haraldkellner widespreadoccurrenceofexpressedfungalsecretoryperoxidasesinforestsoils AT patricialuis widespreadoccurrenceofexpressedfungalsecretoryperoxidasesinforestsoils AT marekjpecyna widespreadoccurrenceofexpressedfungalsecretoryperoxidasesinforestsoils AT florianbarbi widespreadoccurrenceofexpressedfungalsecretoryperoxidasesinforestsoils AT danutakapturska widespreadoccurrenceofexpressedfungalsecretoryperoxidasesinforestsoils AT dirkkruger widespreadoccurrenceofexpressedfungalsecretoryperoxidasesinforestsoils AT donaldrzak widespreadoccurrenceofexpressedfungalsecretoryperoxidasesinforestsoils AT rolandmarmeisse widespreadoccurrenceofexpressedfungalsecretoryperoxidasesinforestsoils AT michelinevandenbol widespreadoccurrenceofexpressedfungalsecretoryperoxidasesinforestsoils AT martinhofrichter widespreadoccurrenceofexpressedfungalsecretoryperoxidasesinforestsoils |