Heat-Killed <i>Bifidobacterium longum</i> BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model
Many probiotic bacteria have been proven to prevent allergic airway responses through immunomodulation. This study was conducted to evaluate the effects of heat-killed <i>Bifidobacterium longum</i> BBMN68 (BBMN68) in pasteurized yogurt on the alleviation of mugwort pollen (MP)-induced al...
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MDPI AG
2023-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/10/2049 |
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| author | Xiaokang Niu Xindi Yin Xiuying Wu Qi Zhang Yunyun Jiang Jingjing He Yuyang Zhao Chao Zhang Yimei Ren Mengxuan Lai Yue Sang Ran Wang |
| author_facet | Xiaokang Niu Xindi Yin Xiuying Wu Qi Zhang Yunyun Jiang Jingjing He Yuyang Zhao Chao Zhang Yimei Ren Mengxuan Lai Yue Sang Ran Wang |
| author_sort | Xiaokang Niu |
| collection | DOAJ |
| description | Many probiotic bacteria have been proven to prevent allergic airway responses through immunomodulation. This study was conducted to evaluate the effects of heat-killed <i>Bifidobacterium longum</i> BBMN68 (BBMN68) in pasteurized yogurt on the alleviation of mugwort pollen (MP)-induced allergic inflammation. BALB/c mice aged 5–6 weeks were randomly assigned and fed pasteurized yogurt containing heat-killed BBMN68 for 27 days, followed by allergic sensitization and challenge with MP extract. The allergic mice that received pasteurized yogurt containing heat-killed BBMN68 had improved immune status, including a lower serum IgE level, decreased serum interleukin (IL)-4, IL-5, and IL-13 concentrations, and alleviated airway inflammation manifested by increased macrophage and decreased eosinophil and neutrophil counts in BALF, as well as airway remodeling and suppressed peribronchial cellular infiltration. Moreover, oral administration of pasteurized yogurt containing heat-killed BBMN68 significantly modulated gut microbiota composition by influencing the proportion of beneficial genera associated with inflammation and immunity, such as <i>Lactobacillus</i>, <i>Candidatus_Saccharimonas</i>, <i>Odoribacter</i>, and <i>Parabacteroides</i>, which also negatively correlated with serum IgE and Th2 cytokine levels. These results demonstrated that pasteurized yogurt containing heat-killed BBMN68 had mitigative effects on allergic airway inflammation, likely through maintaining the systemic Th1/Th2 immune balance by altering the structure and function of the gut microbiota. |
| first_indexed | 2024-03-11T03:43:52Z |
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| id | doaj.art-a8c00fd657044c7db77ec46676c3048a |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T03:43:52Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-a8c00fd657044c7db77ec46676c3048a2023-11-18T01:22:14ZengMDPI AGFoods2304-81582023-05-011210204910.3390/foods12102049Heat-Killed <i>Bifidobacterium longum</i> BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine ModelXiaokang Niu0Xindi Yin1Xiuying Wu2Qi Zhang3Yunyun Jiang4Jingjing He5Yuyang Zhao6Chao Zhang7Yimei Ren8Mengxuan Lai9Yue Sang10Ran Wang11Key Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaKey Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaInner Mongolia Mengniu Dairy (Group) Co., Ltd., Hohhot 011500, ChinaKey Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaInner Mongolia Mengniu Dairy (Group) Co., Ltd., Hohhot 011500, ChinaKey Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaCollege of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaCollege of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaInner Mongolia Mengniu Dairy (Group) Co., Ltd., Hohhot 011500, ChinaHebei Engineering Research Center of Animal Product, Sanhe 065200, ChinaKey Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaMany probiotic bacteria have been proven to prevent allergic airway responses through immunomodulation. This study was conducted to evaluate the effects of heat-killed <i>Bifidobacterium longum</i> BBMN68 (BBMN68) in pasteurized yogurt on the alleviation of mugwort pollen (MP)-induced allergic inflammation. BALB/c mice aged 5–6 weeks were randomly assigned and fed pasteurized yogurt containing heat-killed BBMN68 for 27 days, followed by allergic sensitization and challenge with MP extract. The allergic mice that received pasteurized yogurt containing heat-killed BBMN68 had improved immune status, including a lower serum IgE level, decreased serum interleukin (IL)-4, IL-5, and IL-13 concentrations, and alleviated airway inflammation manifested by increased macrophage and decreased eosinophil and neutrophil counts in BALF, as well as airway remodeling and suppressed peribronchial cellular infiltration. Moreover, oral administration of pasteurized yogurt containing heat-killed BBMN68 significantly modulated gut microbiota composition by influencing the proportion of beneficial genera associated with inflammation and immunity, such as <i>Lactobacillus</i>, <i>Candidatus_Saccharimonas</i>, <i>Odoribacter</i>, and <i>Parabacteroides</i>, which also negatively correlated with serum IgE and Th2 cytokine levels. These results demonstrated that pasteurized yogurt containing heat-killed BBMN68 had mitigative effects on allergic airway inflammation, likely through maintaining the systemic Th1/Th2 immune balance by altering the structure and function of the gut microbiota.https://www.mdpi.com/2304-8158/12/10/2049airway allergic inflammation<i>Bifidobacterium longum</i> BBMN68heat-killed bacteriagut microbiotagut-lung axispollen allergy |
| spellingShingle | Xiaokang Niu Xindi Yin Xiuying Wu Qi Zhang Yunyun Jiang Jingjing He Yuyang Zhao Chao Zhang Yimei Ren Mengxuan Lai Yue Sang Ran Wang Heat-Killed <i>Bifidobacterium longum</i> BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model Foods airway allergic inflammation <i>Bifidobacterium longum</i> BBMN68 heat-killed bacteria gut microbiota gut-lung axis pollen allergy |
| title | Heat-Killed <i>Bifidobacterium longum</i> BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model |
| title_full | Heat-Killed <i>Bifidobacterium longum</i> BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model |
| title_fullStr | Heat-Killed <i>Bifidobacterium longum</i> BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model |
| title_full_unstemmed | Heat-Killed <i>Bifidobacterium longum</i> BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model |
| title_short | Heat-Killed <i>Bifidobacterium longum</i> BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model |
| title_sort | heat killed i bifidobacterium longum i bbmn68 in pasteurized yogurt alleviates mugwort pollen induced allergic airway responses through gut microbiota modulation in a murine model |
| topic | airway allergic inflammation <i>Bifidobacterium longum</i> BBMN68 heat-killed bacteria gut microbiota gut-lung axis pollen allergy |
| url | https://www.mdpi.com/2304-8158/12/10/2049 |
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