Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications
Abstract Background Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. the use of oxidoreductases). Oxidative enzymes from microorganisms offer eco-friendly,...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-05-01
|
| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12896-020-00617-y |
| _version_ | 1828964161598521344 |
|---|---|
| author | Wissal Ben Ali Delphine Chaduli David Navarro Christian Lechat Annick Turbé-Doan Emmanuel Bertrand Craig B. Faulds Giuliano Sciara Laurence Lesage-Meessen Eric Record Tahar Mechichi |
| author_facet | Wissal Ben Ali Delphine Chaduli David Navarro Christian Lechat Annick Turbé-Doan Emmanuel Bertrand Craig B. Faulds Giuliano Sciara Laurence Lesage-Meessen Eric Record Tahar Mechichi |
| author_sort | Wissal Ben Ali |
| collection | DOAJ |
| description | Abstract Background Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. the use of oxidoreductases). Oxidative enzymes from microorganisms offer eco-friendly, cost–effective processes amenable to biotechnological applications, such as in industrial dye decolorization. The aim of this study was to screen marine-derived fungal strains isolated from three coastal areas in Tunisia to identify laccase-like activities, and to produce and characterize active cell-free supernatants of interest for dye decolorization. Results Following the screening of 20 fungal strains isolated from the harbors of Sfax and Monastir (Tunisia), five strains were identified that displayed laccase-like activities. Molecular-based taxonomic approaches identified these strains as belonging to the species Trichoderma asperellum, Stemphylium lucomagnoense and Aspergillus nidulans. Among these five isolates, one T. asperellum strain (T. asperellum 1) gave the highest level of secreted oxidative activities, and so was chosen for further studies. Optimization of the growth medium for liquid cultures was first undertaken to improve the level of laccase-like activity in culture supernatants. Finally, the culture supernatant of T. asperellum 1 decolorized different synthetic dyes belonging to diverse dye families, in the presence or absence of 1-hydroxybenzotriazole (HBT) as a mediator. Conclusions The optimal growth conditions to produce laccase-like active cell-free supernatants from T. asperellum 1 were 1.8 mM CuSO4 as an inducer, 1% NaCl to mimic a seawater environment and 3% sucrose as a carbon source. The culture supernatant of T. asperellum 1 effectively decolorized different synthetic dyes belonging to diverse chemical classes, and the presence of HBT as a mediator improved the decolorization process. |
| first_indexed | 2024-12-14T10:44:32Z |
| format | Article |
| id | doaj.art-a8c435289ec145e8af624916a9f01611 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-14T10:44:32Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-a8c435289ec145e8af624916a9f016112022-12-21T23:05:31ZengBMCBMC Biotechnology1472-67502020-05-0120111310.1186/s12896-020-00617-yScreening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applicationsWissal Ben Ali0Delphine Chaduli1David Navarro2Christian Lechat3Annick Turbé-Doan4Emmanuel Bertrand5Craig B. Faulds6Giuliano Sciara7Laurence Lesage-Meessen8Eric Record9Tahar Mechichi10Ecole Nationale d’Ingénieurs de Sfax, Laboratoire de Biochimie et de Génie enzymatique des lipases, Université de SfaxBiodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRA UMR1163Biodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRA UMR1163AscofranceBiodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRA UMR1163Biodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRA UMR1163Biodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRA UMR1163Biodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRA UMR1163Biodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRA UMR1163Biodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRA UMR1163Ecole Nationale d’Ingénieurs de Sfax, Laboratoire de Biochimie et de Génie enzymatique des lipases, Université de SfaxAbstract Background Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. the use of oxidoreductases). Oxidative enzymes from microorganisms offer eco-friendly, cost–effective processes amenable to biotechnological applications, such as in industrial dye decolorization. The aim of this study was to screen marine-derived fungal strains isolated from three coastal areas in Tunisia to identify laccase-like activities, and to produce and characterize active cell-free supernatants of interest for dye decolorization. Results Following the screening of 20 fungal strains isolated from the harbors of Sfax and Monastir (Tunisia), five strains were identified that displayed laccase-like activities. Molecular-based taxonomic approaches identified these strains as belonging to the species Trichoderma asperellum, Stemphylium lucomagnoense and Aspergillus nidulans. Among these five isolates, one T. asperellum strain (T. asperellum 1) gave the highest level of secreted oxidative activities, and so was chosen for further studies. Optimization of the growth medium for liquid cultures was first undertaken to improve the level of laccase-like activity in culture supernatants. Finally, the culture supernatant of T. asperellum 1 decolorized different synthetic dyes belonging to diverse dye families, in the presence or absence of 1-hydroxybenzotriazole (HBT) as a mediator. Conclusions The optimal growth conditions to produce laccase-like active cell-free supernatants from T. asperellum 1 were 1.8 mM CuSO4 as an inducer, 1% NaCl to mimic a seawater environment and 3% sucrose as a carbon source. The culture supernatant of T. asperellum 1 effectively decolorized different synthetic dyes belonging to diverse chemical classes, and the presence of HBT as a mediator improved the decolorization process.http://link.springer.com/article/10.1186/s12896-020-00617-yMarine-derived fungiTrichoderma asperellumLaccase-like activityLaccaseDyes |
| spellingShingle | Wissal Ben Ali Delphine Chaduli David Navarro Christian Lechat Annick Turbé-Doan Emmanuel Bertrand Craig B. Faulds Giuliano Sciara Laurence Lesage-Meessen Eric Record Tahar Mechichi Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications BMC Biotechnology Marine-derived fungi Trichoderma asperellum Laccase-like activity Laccase Dyes |
| title | Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications |
| title_full | Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications |
| title_fullStr | Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications |
| title_full_unstemmed | Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications |
| title_short | Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications |
| title_sort | screening of five marine derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications |
| topic | Marine-derived fungi Trichoderma asperellum Laccase-like activity Laccase Dyes |
| url | http://link.springer.com/article/10.1186/s12896-020-00617-y |
| work_keys_str_mv | AT wissalbenali screeningoffivemarinederivedfungalstrainsfortheirpotentialtoproduceoxidaseswithlaccaseactivitiessuitableforbiotechnologicalapplications AT delphinechaduli screeningoffivemarinederivedfungalstrainsfortheirpotentialtoproduceoxidaseswithlaccaseactivitiessuitableforbiotechnologicalapplications AT davidnavarro screeningoffivemarinederivedfungalstrainsfortheirpotentialtoproduceoxidaseswithlaccaseactivitiessuitableforbiotechnologicalapplications AT christianlechat screeningoffivemarinederivedfungalstrainsfortheirpotentialtoproduceoxidaseswithlaccaseactivitiessuitableforbiotechnologicalapplications AT annickturbedoan screeningoffivemarinederivedfungalstrainsfortheirpotentialtoproduceoxidaseswithlaccaseactivitiessuitableforbiotechnologicalapplications AT emmanuelbertrand screeningoffivemarinederivedfungalstrainsfortheirpotentialtoproduceoxidaseswithlaccaseactivitiessuitableforbiotechnologicalapplications AT craigbfaulds screeningoffivemarinederivedfungalstrainsfortheirpotentialtoproduceoxidaseswithlaccaseactivitiessuitableforbiotechnologicalapplications AT giulianosciara screeningoffivemarinederivedfungalstrainsfortheirpotentialtoproduceoxidaseswithlaccaseactivitiessuitableforbiotechnologicalapplications AT laurencelesagemeessen screeningoffivemarinederivedfungalstrainsfortheirpotentialtoproduceoxidaseswithlaccaseactivitiessuitableforbiotechnologicalapplications AT ericrecord screeningoffivemarinederivedfungalstrainsfortheirpotentialtoproduceoxidaseswithlaccaseactivitiessuitableforbiotechnologicalapplications AT taharmechichi screeningoffivemarinederivedfungalstrainsfortheirpotentialtoproduceoxidaseswithlaccaseactivitiessuitableforbiotechnologicalapplications |