| Summary: | Probiotic bacteria are receiving increased attention due to the potential benefits to their hosts. Plateau yaks have resistance against diseases and stress, which is potentially related to their inner probiotics. To uncover the potential functional genes of yak probiotics, we sequenced the whole genome of <i>Lactobacillus sakei</i> (<i>L. sakei</i>). The results showed that the genome length of <i>L. sakei</i> was 1.99 Mbp, with 1943 protein coding genes (21 rRNA, 65 tRNA, and 1 tmRNA). There were three plasmids found in this bacteria, with 88 protein coding genes. EggNOG annotation uncovered that the <i>L. sakei</i> genes were found to belong to J (translation, ribosomal structure, and biogenesis), L (replication, recombination, and repair), G (carbohydrate transport and metabolism), and K (transcription). GO annotation showed that most of the <i>L. sakei</i> genes were related to cellular processes, metabolic processes, biological regulation, localization, response to stimulus, and organization or biogenesis of cellular components. CAZy annotation found that there were 123 CAZys in the <i>L. sakei</i> genome, with glycosyl transferases and glycoside hydrolases. Our results revealed the genome characteristics of <i>L. sakei</i>, which may give insight into the future employment of this probiotic bacterium for its functional benefits.
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