The Complete Genome of Probiotic <i>Lactobacillus sakei</i> Derived from Plateau Yak Feces
Probiotic bacteria are receiving increased attention due to the potential benefits to their hosts. Plateau yaks have resistance against diseases and stress, which is potentially related to their inner probiotics. To uncover the potential functional genes of yak probiotics, we sequenced the whole gen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/12/1527 |
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| author | Kun Li Juanjuan Liu Zhibo Zeng Muhammad Fakhar-e-Alam Kulyar Yaping Wang Aoyun Li Zeeshan Ahmad Bhutta Amjad Islam Aqib Muhammad Shahzad Jiakui Li Desheng Qi |
| author_facet | Kun Li Juanjuan Liu Zhibo Zeng Muhammad Fakhar-e-Alam Kulyar Yaping Wang Aoyun Li Zeeshan Ahmad Bhutta Amjad Islam Aqib Muhammad Shahzad Jiakui Li Desheng Qi |
| author_sort | Kun Li |
| collection | DOAJ |
| description | Probiotic bacteria are receiving increased attention due to the potential benefits to their hosts. Plateau yaks have resistance against diseases and stress, which is potentially related to their inner probiotics. To uncover the potential functional genes of yak probiotics, we sequenced the whole genome of <i>Lactobacillus sakei</i> (<i>L. sakei</i>). The results showed that the genome length of <i>L. sakei</i> was 1.99 Mbp, with 1943 protein coding genes (21 rRNA, 65 tRNA, and 1 tmRNA). There were three plasmids found in this bacteria, with 88 protein coding genes. EggNOG annotation uncovered that the <i>L. sakei</i> genes were found to belong to J (translation, ribosomal structure, and biogenesis), L (replication, recombination, and repair), G (carbohydrate transport and metabolism), and K (transcription). GO annotation showed that most of the <i>L. sakei</i> genes were related to cellular processes, metabolic processes, biological regulation, localization, response to stimulus, and organization or biogenesis of cellular components. CAZy annotation found that there were 123 CAZys in the <i>L. sakei</i> genome, with glycosyl transferases and glycoside hydrolases. Our results revealed the genome characteristics of <i>L. sakei</i>, which may give insight into the future employment of this probiotic bacterium for its functional benefits. |
| first_indexed | 2024-03-10T13:53:38Z |
| format | Article |
| id | doaj.art-a8ca5b024bfe461b9a5625444904f682 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T13:53:38Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-a8ca5b024bfe461b9a5625444904f6822023-11-21T01:52:22ZengMDPI AGGenes2073-44252020-12-011112152710.3390/genes11121527The Complete Genome of Probiotic <i>Lactobacillus sakei</i> Derived from Plateau Yak FecesKun Li0Juanjuan Liu1Zhibo Zeng2Muhammad Fakhar-e-Alam Kulyar3Yaping Wang4Aoyun Li5Zeeshan Ahmad Bhutta6Amjad Islam Aqib7Muhammad Shahzad8Jiakui Li9Desheng Qi10College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaThe Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Midlothian EH25 9RG, UKDepartment of Medicine Cholistan, University of Veterinary & Animal Sciences, Bahawalpur 64100, PakistanDepartment of Pathology, Faculty of Veterinary & Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaProbiotic bacteria are receiving increased attention due to the potential benefits to their hosts. Plateau yaks have resistance against diseases and stress, which is potentially related to their inner probiotics. To uncover the potential functional genes of yak probiotics, we sequenced the whole genome of <i>Lactobacillus sakei</i> (<i>L. sakei</i>). The results showed that the genome length of <i>L. sakei</i> was 1.99 Mbp, with 1943 protein coding genes (21 rRNA, 65 tRNA, and 1 tmRNA). There were three plasmids found in this bacteria, with 88 protein coding genes. EggNOG annotation uncovered that the <i>L. sakei</i> genes were found to belong to J (translation, ribosomal structure, and biogenesis), L (replication, recombination, and repair), G (carbohydrate transport and metabolism), and K (transcription). GO annotation showed that most of the <i>L. sakei</i> genes were related to cellular processes, metabolic processes, biological regulation, localization, response to stimulus, and organization or biogenesis of cellular components. CAZy annotation found that there were 123 CAZys in the <i>L. sakei</i> genome, with glycosyl transferases and glycoside hydrolases. Our results revealed the genome characteristics of <i>L. sakei</i>, which may give insight into the future employment of this probiotic bacterium for its functional benefits.https://www.mdpi.com/2073-4425/11/12/1527probioticyak<i>Lactobacillus sakei</i>genomesequencing |
| spellingShingle | Kun Li Juanjuan Liu Zhibo Zeng Muhammad Fakhar-e-Alam Kulyar Yaping Wang Aoyun Li Zeeshan Ahmad Bhutta Amjad Islam Aqib Muhammad Shahzad Jiakui Li Desheng Qi The Complete Genome of Probiotic <i>Lactobacillus sakei</i> Derived from Plateau Yak Feces Genes probiotic yak <i>Lactobacillus sakei</i> genome sequencing |
| title | The Complete Genome of Probiotic <i>Lactobacillus sakei</i> Derived from Plateau Yak Feces |
| title_full | The Complete Genome of Probiotic <i>Lactobacillus sakei</i> Derived from Plateau Yak Feces |
| title_fullStr | The Complete Genome of Probiotic <i>Lactobacillus sakei</i> Derived from Plateau Yak Feces |
| title_full_unstemmed | The Complete Genome of Probiotic <i>Lactobacillus sakei</i> Derived from Plateau Yak Feces |
| title_short | The Complete Genome of Probiotic <i>Lactobacillus sakei</i> Derived from Plateau Yak Feces |
| title_sort | complete genome of probiotic i lactobacillus sakei i derived from plateau yak feces |
| topic | probiotic yak <i>Lactobacillus sakei</i> genome sequencing |
| url | https://www.mdpi.com/2073-4425/11/12/1527 |
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