Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility
Abstract Background Expression of glycosyl hydrolases in lignocellulosic biomass has been proposed as an alternative to improve efficiency of cellulosic ethanol production. In planta production of hyperthermophilic hydrolytic enzymes could prevent the detrimental effects often seen resulting from th...
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| Format: | Article |
| Language: | English |
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BMC
2018-08-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-018-1224-7 |
| _version_ | 1818265290381721600 |
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| author | Yao Xiao Xuejun He Yemaiza Ojeda-Lassalle Charleson Poovaiah Heather D. Coleman |
| author_facet | Yao Xiao Xuejun He Yemaiza Ojeda-Lassalle Charleson Poovaiah Heather D. Coleman |
| author_sort | Yao Xiao |
| collection | DOAJ |
| description | Abstract Background Expression of glycosyl hydrolases in lignocellulosic biomass has been proposed as an alternative to improve efficiency of cellulosic ethanol production. In planta production of hyperthermophilic hydrolytic enzymes could prevent the detrimental effects often seen resulting from the expression of recombinant mesophilic enzymes to plant hosts. Utilizing lignocellulosic feedstocks to produce hyperthermophilic hydrolases provides additional benefits for ethanol production in the way of transgenic feedstocks serving as both enzyme providers and cellulosic substrates. Results In this study, transgenic hybrid poplar (Populus alba × grandidentata) was generated to express a hyperthermophilic endoglucanase from Thermotoga neapolitana with an optimal temperature over 100 °C. Functional hyperthermoactive endoglucanase was successfully produced in the transgenic events, and altered phenotypic growth was observed in transgenic lines. Moreover, the line with the highest TnCelB expression in both leaf and developing xylem had reduced lignin content and cellulose crystallinity, resulting in a more digestible cell wall. The activation of TnCelB by a post-harvest heat treatment resulted in enhanced saccharification efficiencies of transgenic poplar lines with moderate TnCelB expression and without alteration of cellulose and lignin when not heat-treated. In planta high-level overexpression of a hyperthermophilic endoglucanase paired with heat treatment following harvest, resulted in biomass that was comparable with wild-type lines that underwent a traditional pretreatment for saccharification. Conclusions Overexpression of hyperthermophilic endoglucanase in feedstock had impacts on plant growth and cell wall composition, especially when the enzyme was highly expressed. Improved glucan saccharification efficiencies from transgenic lines before and after heat treatment could reduce both the economic and environmental costs associated with ethanol production from lignocellulosic biomass. |
| first_indexed | 2024-12-12T19:48:27Z |
| format | Article |
| id | doaj.art-a8ce16936e0d475abf29bbc1fe9285bf |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T19:48:27Z |
| publishDate | 2018-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-a8ce16936e0d475abf29bbc1fe9285bf2022-12-22T00:14:02ZengBMCBiotechnology for Biofuels1754-68342018-08-0111111510.1186/s13068-018-1224-7Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibilityYao Xiao0Xuejun He1Yemaiza Ojeda-Lassalle2Charleson Poovaiah3Heather D. Coleman4Biology Department, Syracuse UniversityBiology Department, Syracuse UniversityBiology Department, Syracuse UniversityBiology Department, Syracuse UniversityBiology Department, Syracuse UniversityAbstract Background Expression of glycosyl hydrolases in lignocellulosic biomass has been proposed as an alternative to improve efficiency of cellulosic ethanol production. In planta production of hyperthermophilic hydrolytic enzymes could prevent the detrimental effects often seen resulting from the expression of recombinant mesophilic enzymes to plant hosts. Utilizing lignocellulosic feedstocks to produce hyperthermophilic hydrolases provides additional benefits for ethanol production in the way of transgenic feedstocks serving as both enzyme providers and cellulosic substrates. Results In this study, transgenic hybrid poplar (Populus alba × grandidentata) was generated to express a hyperthermophilic endoglucanase from Thermotoga neapolitana with an optimal temperature over 100 °C. Functional hyperthermoactive endoglucanase was successfully produced in the transgenic events, and altered phenotypic growth was observed in transgenic lines. Moreover, the line with the highest TnCelB expression in both leaf and developing xylem had reduced lignin content and cellulose crystallinity, resulting in a more digestible cell wall. The activation of TnCelB by a post-harvest heat treatment resulted in enhanced saccharification efficiencies of transgenic poplar lines with moderate TnCelB expression and without alteration of cellulose and lignin when not heat-treated. In planta high-level overexpression of a hyperthermophilic endoglucanase paired with heat treatment following harvest, resulted in biomass that was comparable with wild-type lines that underwent a traditional pretreatment for saccharification. Conclusions Overexpression of hyperthermophilic endoglucanase in feedstock had impacts on plant growth and cell wall composition, especially when the enzyme was highly expressed. Improved glucan saccharification efficiencies from transgenic lines before and after heat treatment could reduce both the economic and environmental costs associated with ethanol production from lignocellulosic biomass.http://link.springer.com/article/10.1186/s13068-018-1224-7Hyperthermophilic cellulaseTransgenic poplarCell wall compositionSaccharificationBiofuel production |
| spellingShingle | Yao Xiao Xuejun He Yemaiza Ojeda-Lassalle Charleson Poovaiah Heather D. Coleman Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility Biotechnology for Biofuels Hyperthermophilic cellulase Transgenic poplar Cell wall composition Saccharification Biofuel production |
| title | Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility |
| title_full | Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility |
| title_fullStr | Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility |
| title_full_unstemmed | Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility |
| title_short | Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility |
| title_sort | expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility |
| topic | Hyperthermophilic cellulase Transgenic poplar Cell wall composition Saccharification Biofuel production |
| url | http://link.springer.com/article/10.1186/s13068-018-1224-7 |
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