Transcriptome Analysis of the Responses of Rice Leaves to Chilling and Subsequent Recovery
Improving chilling tolerance at the seedling stage in rice is essential for agricultural research. We combined a physiological analysis with transcriptomics in a variety Dular subjected to chilling followed by recovery at normal temperature to better understand the chilling tolerance mechanisms of r...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/18/10739 |
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| author | Zhong Li Muhammad Umar Khan Puleng Letuma Yuebin Xie Wenshan Zhan Wei Wang Yuhang Jiang Wenxiong Lin Zhixing Zhang |
| author_facet | Zhong Li Muhammad Umar Khan Puleng Letuma Yuebin Xie Wenshan Zhan Wei Wang Yuhang Jiang Wenxiong Lin Zhixing Zhang |
| author_sort | Zhong Li |
| collection | DOAJ |
| description | Improving chilling tolerance at the seedling stage in rice is essential for agricultural research. We combined a physiological analysis with transcriptomics in a variety Dular subjected to chilling followed by recovery at normal temperature to better understand the chilling tolerance mechanisms of rice. Chilling inhibited the synthesis of chlorophyll and non-structural carbohydrate (NSC) and disrupted the ion balance of the plant, resulting in the impaired function of rice leaves. The recovery treatment can effectively reverse the chilling-related injury. Transcriptome results displayed that 21,970 genes were identified at three different temperatures, and 11,732 genes were differentially expressed. According to KEGG analysis, functional categories for differentially expressed genes (DEGs) mainly included ribosome (8.72%), photosynthesis–antenna proteins (7.38%), phenylpropanoid biosynthesis (11.41%), and linoleic acid metabolism (10.07%). The subcellular localization demonstrated that most proteins were located in the chloroplasts (29.30%), cytosol (10.19%), and nucleus (10.19%). We proposed that some genes involved in photosynthesis, ribosome, phenylpropanoid biosynthesis, and linoleic acid metabolism may play key roles in enhancing rice adaptation to chilling stress and their recovery capacity. These findings provide a foundation for future research into rice chilling tolerance mechanisms. |
| first_indexed | 2024-03-09T23:44:03Z |
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| id | doaj.art-a8d0da80ed304464aac73db47e3584c6 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:44:03Z |
| publishDate | 2022-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a8d0da80ed304464aac73db47e3584c62023-11-23T16:47:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181073910.3390/ijms231810739Transcriptome Analysis of the Responses of Rice Leaves to Chilling and Subsequent RecoveryZhong Li0Muhammad Umar Khan1Puleng Letuma2Yuebin Xie3Wenshan Zhan4Wei Wang5Yuhang Jiang6Wenxiong Lin7Zhixing Zhang8Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCrop Science Department, Faculty of Agriculture, National University of Lesotho, Maseru 100, LesothoFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaImproving chilling tolerance at the seedling stage in rice is essential for agricultural research. We combined a physiological analysis with transcriptomics in a variety Dular subjected to chilling followed by recovery at normal temperature to better understand the chilling tolerance mechanisms of rice. Chilling inhibited the synthesis of chlorophyll and non-structural carbohydrate (NSC) and disrupted the ion balance of the plant, resulting in the impaired function of rice leaves. The recovery treatment can effectively reverse the chilling-related injury. Transcriptome results displayed that 21,970 genes were identified at three different temperatures, and 11,732 genes were differentially expressed. According to KEGG analysis, functional categories for differentially expressed genes (DEGs) mainly included ribosome (8.72%), photosynthesis–antenna proteins (7.38%), phenylpropanoid biosynthesis (11.41%), and linoleic acid metabolism (10.07%). The subcellular localization demonstrated that most proteins were located in the chloroplasts (29.30%), cytosol (10.19%), and nucleus (10.19%). We proposed that some genes involved in photosynthesis, ribosome, phenylpropanoid biosynthesis, and linoleic acid metabolism may play key roles in enhancing rice adaptation to chilling stress and their recovery capacity. These findings provide a foundation for future research into rice chilling tolerance mechanisms.https://www.mdpi.com/1422-0067/23/18/10739temperature variation<i>Oryza sativa</i> L.transcriptional regulationion balancegrowth and developmentadversity stress |
| spellingShingle | Zhong Li Muhammad Umar Khan Puleng Letuma Yuebin Xie Wenshan Zhan Wei Wang Yuhang Jiang Wenxiong Lin Zhixing Zhang Transcriptome Analysis of the Responses of Rice Leaves to Chilling and Subsequent Recovery International Journal of Molecular Sciences temperature variation <i>Oryza sativa</i> L. transcriptional regulation ion balance growth and development adversity stress |
| title | Transcriptome Analysis of the Responses of Rice Leaves to Chilling and Subsequent Recovery |
| title_full | Transcriptome Analysis of the Responses of Rice Leaves to Chilling and Subsequent Recovery |
| title_fullStr | Transcriptome Analysis of the Responses of Rice Leaves to Chilling and Subsequent Recovery |
| title_full_unstemmed | Transcriptome Analysis of the Responses of Rice Leaves to Chilling and Subsequent Recovery |
| title_short | Transcriptome Analysis of the Responses of Rice Leaves to Chilling and Subsequent Recovery |
| title_sort | transcriptome analysis of the responses of rice leaves to chilling and subsequent recovery |
| topic | temperature variation <i>Oryza sativa</i> L. transcriptional regulation ion balance growth and development adversity stress |
| url | https://www.mdpi.com/1422-0067/23/18/10739 |
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