Rotations with Indian Mustard and Wild Rocket Suppressed Cucumber Fusarium Wilt Disease and Changed Rhizosphere Bacterial Communities
Crop monocropping usually results in an enrichment of soil-borne pathogens in soil. Crop rotation is an environmentally friendly method for controlling soil-borne diseases. Plant rhizosphere microorganisms, especially plant-beneficial microorganisms, play a major role in protecting plants from patho...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-02-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/7/2/57 |
| _version_ | 1818268075965808640 |
|---|---|
| author | Xue Jin Jian Wang Dalong Li Fengzhi Wu Xingang Zhou |
| author_facet | Xue Jin Jian Wang Dalong Li Fengzhi Wu Xingang Zhou |
| author_sort | Xue Jin |
| collection | DOAJ |
| description | Crop monocropping usually results in an enrichment of soil-borne pathogens in soil. Crop rotation is an environmentally friendly method for controlling soil-borne diseases. Plant rhizosphere microorganisms, especially plant-beneficial microorganisms, play a major role in protecting plants from pathogens, but responses of these microorganisms to crop rotation remain unclear. Here, we evaluated the effects of rotations with Indian mustard (<i>Brassica juncea</i>) and wild rocket (<i>Diplotaxis tenuifolia</i> (L.) DC.) on cucumber Fusarium wilt disease caused by <i>Fusarium oxysporum</i> f.sp. <i>cucumerinum</i> (FOC). Cucumber rhizosphere bacterial community composition was analyzed by high-throughput amplicon sequencing. Bacteria, <i>Pseudomonas</i> spp., 2,4-diacetylphloroglucinol (an antifungal secondary metabolite) producer and FOC abundances were estimated by real-time PCR. Rotations with Indian mustard and wild rocket suppressed cucumber Fusarium wilt disease and cucumber rhizosphere FOC abundance. Crop rotations increased cucumber rhizosphere bacteria, <i>Pseudomonas</i> spp. and 2,4-diacetylphloroglucinol producer abundances. Moreover, crop rotations changed cucumber rhizosphere bacterial community composition and increased bacterial community diversity. However, crop rotations decreased soil inorganic nitrogen content and inhibited cucumber seedling growth. Overall, rotations with Indian mustard and wild rocket suppressed cucumber Fusarium wilt disease, which might be linked to the increased rhizosphere bacterial diversity and abundances of potential plant-beneficial microorganisms (such as <i>Pseudomonas</i> spp. and 2,4-diacetylphloroglucinol producer). |
| first_indexed | 2024-12-12T20:32:44Z |
| format | Article |
| id | doaj.art-a8d153fe92814fc3a573588702ff7c52 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-12T20:32:44Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a8d153fe92814fc3a573588702ff7c522022-12-22T00:12:59ZengMDPI AGMicroorganisms2076-26072019-02-01725710.3390/microorganisms7020057microorganisms7020057Rotations with Indian Mustard and Wild Rocket Suppressed Cucumber Fusarium Wilt Disease and Changed Rhizosphere Bacterial CommunitiesXue Jin0Jian Wang1Dalong Li2Fengzhi Wu3Xingang Zhou4Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaDepartment of Horticulture, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaCrop monocropping usually results in an enrichment of soil-borne pathogens in soil. Crop rotation is an environmentally friendly method for controlling soil-borne diseases. Plant rhizosphere microorganisms, especially plant-beneficial microorganisms, play a major role in protecting plants from pathogens, but responses of these microorganisms to crop rotation remain unclear. Here, we evaluated the effects of rotations with Indian mustard (<i>Brassica juncea</i>) and wild rocket (<i>Diplotaxis tenuifolia</i> (L.) DC.) on cucumber Fusarium wilt disease caused by <i>Fusarium oxysporum</i> f.sp. <i>cucumerinum</i> (FOC). Cucumber rhizosphere bacterial community composition was analyzed by high-throughput amplicon sequencing. Bacteria, <i>Pseudomonas</i> spp., 2,4-diacetylphloroglucinol (an antifungal secondary metabolite) producer and FOC abundances were estimated by real-time PCR. Rotations with Indian mustard and wild rocket suppressed cucumber Fusarium wilt disease and cucumber rhizosphere FOC abundance. Crop rotations increased cucumber rhizosphere bacteria, <i>Pseudomonas</i> spp. and 2,4-diacetylphloroglucinol producer abundances. Moreover, crop rotations changed cucumber rhizosphere bacterial community composition and increased bacterial community diversity. However, crop rotations decreased soil inorganic nitrogen content and inhibited cucumber seedling growth. Overall, rotations with Indian mustard and wild rocket suppressed cucumber Fusarium wilt disease, which might be linked to the increased rhizosphere bacterial diversity and abundances of potential plant-beneficial microorganisms (such as <i>Pseudomonas</i> spp. and 2,4-diacetylphloroglucinol producer).https://www.mdpi.com/2076-2607/7/2/57bacterial community compositioncrop rotation<i>Cucumis sativus</i> L.fusarium wilt<i>Pseudomonas</i> spp |
| spellingShingle | Xue Jin Jian Wang Dalong Li Fengzhi Wu Xingang Zhou Rotations with Indian Mustard and Wild Rocket Suppressed Cucumber Fusarium Wilt Disease and Changed Rhizosphere Bacterial Communities Microorganisms bacterial community composition crop rotation <i>Cucumis sativus</i> L. fusarium wilt <i>Pseudomonas</i> spp |
| title | Rotations with Indian Mustard and Wild Rocket Suppressed Cucumber Fusarium Wilt Disease and Changed Rhizosphere Bacterial Communities |
| title_full | Rotations with Indian Mustard and Wild Rocket Suppressed Cucumber Fusarium Wilt Disease and Changed Rhizosphere Bacterial Communities |
| title_fullStr | Rotations with Indian Mustard and Wild Rocket Suppressed Cucumber Fusarium Wilt Disease and Changed Rhizosphere Bacterial Communities |
| title_full_unstemmed | Rotations with Indian Mustard and Wild Rocket Suppressed Cucumber Fusarium Wilt Disease and Changed Rhizosphere Bacterial Communities |
| title_short | Rotations with Indian Mustard and Wild Rocket Suppressed Cucumber Fusarium Wilt Disease and Changed Rhizosphere Bacterial Communities |
| title_sort | rotations with indian mustard and wild rocket suppressed cucumber fusarium wilt disease and changed rhizosphere bacterial communities |
| topic | bacterial community composition crop rotation <i>Cucumis sativus</i> L. fusarium wilt <i>Pseudomonas</i> spp |
| url | https://www.mdpi.com/2076-2607/7/2/57 |
| work_keys_str_mv | AT xuejin rotationswithindianmustardandwildrocketsuppressedcucumberfusariumwiltdiseaseandchangedrhizospherebacterialcommunities AT jianwang rotationswithindianmustardandwildrocketsuppressedcucumberfusariumwiltdiseaseandchangedrhizospherebacterialcommunities AT dalongli rotationswithindianmustardandwildrocketsuppressedcucumberfusariumwiltdiseaseandchangedrhizospherebacterialcommunities AT fengzhiwu rotationswithindianmustardandwildrocketsuppressedcucumberfusariumwiltdiseaseandchangedrhizospherebacterialcommunities AT xingangzhou rotationswithindianmustardandwildrocketsuppressedcucumberfusariumwiltdiseaseandchangedrhizospherebacterialcommunities |