Identification of squalamine in the plasma membrane of white blood cells in the sea lamprey, Petromyzon marinus
It is well established that innate mechanisms play an important role in the immunity of fish. Antimicrobial peptides have been isolated and characterized from several species of teleosts. Here, we report the isolation of an antimicrobial compound from the blood of bacterially challenged sea lamprey,...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2007-12-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520429086 |
| _version_ | 1818934015760007168 |
|---|---|
| author | Sang-Seon Yun Weiming Li |
| author_facet | Sang-Seon Yun Weiming Li |
| author_sort | Sang-Seon Yun |
| collection | DOAJ |
| description | It is well established that innate mechanisms play an important role in the immunity of fish. Antimicrobial peptides have been isolated and characterized from several species of teleosts. Here, we report the isolation of an antimicrobial compound from the blood of bacterially challenged sea lamprey, Petromyzon marinus. An acetic acid extract from the blood cells of challenged fish was subjected to solid-phase extraction, cation-exchange chromatography, gel-filtration chromatography, and reverse-phase high-performance liquid chromatography, with the purified fractions assayed for antimicrobial activity. Surprisingly, antimicrobial activity in these fractions originated from squalamine, an aminosterol previously identified in the dogfish shark, Squalus acanthias. Further chromatographic and mass spectrometric analyses confirmed the identity of squalamine, an antimicrobial and antiangiogenic agent, in the active fraction from the sea lamprey blood cells. Immunocytochemical analysis localized squalamine to the plasma membrane of white blood cells. Therefore, we postulate that squalamine has an important role in the innate immunity that defends the lamprey against microbial invasion. The full biochemical and immunological roles of squalamine in the white blood cell membrane remain to be investigated. |
| first_indexed | 2024-12-20T04:57:34Z |
| format | Article |
| id | doaj.art-a8d158adf56f42b995d638e38aab8fbd |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T04:57:34Z |
| publishDate | 2007-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-a8d158adf56f42b995d638e38aab8fbd2022-12-21T19:52:40ZengElsevierJournal of Lipid Research0022-22752007-12-01481225792586Identification of squalamine in the plasma membrane of white blood cells in the sea lamprey, Petromyzon marinusSang-Seon Yun0Weiming Li1Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824It is well established that innate mechanisms play an important role in the immunity of fish. Antimicrobial peptides have been isolated and characterized from several species of teleosts. Here, we report the isolation of an antimicrobial compound from the blood of bacterially challenged sea lamprey, Petromyzon marinus. An acetic acid extract from the blood cells of challenged fish was subjected to solid-phase extraction, cation-exchange chromatography, gel-filtration chromatography, and reverse-phase high-performance liquid chromatography, with the purified fractions assayed for antimicrobial activity. Surprisingly, antimicrobial activity in these fractions originated from squalamine, an aminosterol previously identified in the dogfish shark, Squalus acanthias. Further chromatographic and mass spectrometric analyses confirmed the identity of squalamine, an antimicrobial and antiangiogenic agent, in the active fraction from the sea lamprey blood cells. Immunocytochemical analysis localized squalamine to the plasma membrane of white blood cells. Therefore, we postulate that squalamine has an important role in the innate immunity that defends the lamprey against microbial invasion. The full biochemical and immunological roles of squalamine in the white blood cell membrane remain to be investigated.http://www.sciencedirect.com/science/article/pii/S0022227520429086aminosterolinnate immunityneutrophils |
| spellingShingle | Sang-Seon Yun Weiming Li Identification of squalamine in the plasma membrane of white blood cells in the sea lamprey, Petromyzon marinus Journal of Lipid Research aminosterol innate immunity neutrophils |
| title | Identification of squalamine in the plasma membrane of white blood cells in the sea lamprey, Petromyzon marinus |
| title_full | Identification of squalamine in the plasma membrane of white blood cells in the sea lamprey, Petromyzon marinus |
| title_fullStr | Identification of squalamine in the plasma membrane of white blood cells in the sea lamprey, Petromyzon marinus |
| title_full_unstemmed | Identification of squalamine in the plasma membrane of white blood cells in the sea lamprey, Petromyzon marinus |
| title_short | Identification of squalamine in the plasma membrane of white blood cells in the sea lamprey, Petromyzon marinus |
| title_sort | identification of squalamine in the plasma membrane of white blood cells in the sea lamprey petromyzon marinus |
| topic | aminosterol innate immunity neutrophils |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520429086 |
| work_keys_str_mv | AT sangseonyun identificationofsqualamineintheplasmamembraneofwhitebloodcellsinthesealampreypetromyzonmarinus AT weimingli identificationofsqualamineintheplasmamembraneofwhitebloodcellsinthesealampreypetromyzonmarinus |