Comparison of MRI T1, T2, and T2* mapping with histology for assessment of intervertebral disc degeneration in an ovine model
Abstract An easy, reliable, and time-efficient standardized approach for assessing lumbar intervertebral disc (IVD) degeneration with relaxation times measurements in pre-clinical and clinical studies is lacking. This prospective study aims to determine the most appropriate method for lumbar IVD deg...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-09348-w |
| _version_ | 1819025865531457536 |
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| author | Nora Bouhsina Cyrille Decante Jean-Baptiste Hardel Dominique Rouleau Jérôme Abadie Antoine Hamel Catherine Le Visage Julie Lesoeur Jérôme Guicheux Johann Clouet Marion Fusellier |
| author_facet | Nora Bouhsina Cyrille Decante Jean-Baptiste Hardel Dominique Rouleau Jérôme Abadie Antoine Hamel Catherine Le Visage Julie Lesoeur Jérôme Guicheux Johann Clouet Marion Fusellier |
| author_sort | Nora Bouhsina |
| collection | DOAJ |
| description | Abstract An easy, reliable, and time-efficient standardized approach for assessing lumbar intervertebral disc (IVD) degeneration with relaxation times measurements in pre-clinical and clinical studies is lacking. This prospective study aims to determine the most appropriate method for lumbar IVD degeneration (IDD) assessment in sheep by comparing three quantitative MRI sequences (variable-flip-angle T1 mapping, and multi-echo T2 and T2* mapping), correlating them with Pfirrmann grading and histology. Strong intra- and interrater agreements were found for Nucleus pulposus (NP) regions-of-interest (ROI). T1, T2, and T2* mapping correlated with Pfirrmann grading and histological scoring (p < 0.05) except for the most ventral rectangular ROI on T2 maps. Correlations were excellent for all of the T1 ROIs and the T2* NP ROIs. Highly significant differences in T1 values were found between all Pfirrmann grades except between grades I/II and between grades III/IV. Significant differences were identified in the T2 and the T2* values between all grades except between grades I/III. T1, T2, and T2* relaxation times measurements of the NP are an accurate and time-efficient tool to assess lumbar IDD in sheep. Variable-flip-angle T1 mapping may be further considered as a valuable method to investigate IDD and to assess the efficacy of regenerative treatments in longitudinal studies. |
| first_indexed | 2024-12-21T05:17:28Z |
| format | Article |
| id | doaj.art-a8d1b7e266144b0ca22f04d62481a7ea |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T05:17:28Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a8d1b7e266144b0ca22f04d62481a7ea2022-12-21T19:14:53ZengNature PortfolioScientific Reports2045-23222022-03-0112111210.1038/s41598-022-09348-wComparison of MRI T1, T2, and T2* mapping with histology for assessment of intervertebral disc degeneration in an ovine modelNora Bouhsina0Cyrille Decante1Jean-Baptiste Hardel2Dominique Rouleau3Jérôme Abadie4Antoine Hamel5Catherine Le Visage6Julie Lesoeur7Jérôme Guicheux8Johann Clouet9Marion Fusellier10INSERM, UMRS 1229, Regenerative Medicine and Skeleton (RMeS), Université de Nantes, ONIRISINSERM, UMRS 1229, Regenerative Medicine and Skeleton (RMeS), Université de Nantes, ONIRISDepartment of Diagnostic Imaging, CRIP, ONIRIS, College of Veterinary Medicine, Food Science and EngineeringINSERM, UMRS 1229, Regenerative Medicine and Skeleton (RMeS), Université de Nantes, ONIRISLaboniris, ONIRIS, College of Veterinary Medicine, Food Science and EngineeringINSERM, UMRS 1229, Regenerative Medicine and Skeleton (RMeS), Université de Nantes, ONIRISINSERM, UMRS 1229, Regenerative Medicine and Skeleton (RMeS), Université de Nantes, ONIRISINSERM, UMRS 1229, Regenerative Medicine and Skeleton (RMeS), Université de Nantes, ONIRISINSERM, UMRS 1229, Regenerative Medicine and Skeleton (RMeS), Université de Nantes, ONIRISINSERM, UMRS 1229, Regenerative Medicine and Skeleton (RMeS), Université de Nantes, ONIRISINSERM, UMRS 1229, Regenerative Medicine and Skeleton (RMeS), Université de Nantes, ONIRISAbstract An easy, reliable, and time-efficient standardized approach for assessing lumbar intervertebral disc (IVD) degeneration with relaxation times measurements in pre-clinical and clinical studies is lacking. This prospective study aims to determine the most appropriate method for lumbar IVD degeneration (IDD) assessment in sheep by comparing three quantitative MRI sequences (variable-flip-angle T1 mapping, and multi-echo T2 and T2* mapping), correlating them with Pfirrmann grading and histology. Strong intra- and interrater agreements were found for Nucleus pulposus (NP) regions-of-interest (ROI). T1, T2, and T2* mapping correlated with Pfirrmann grading and histological scoring (p < 0.05) except for the most ventral rectangular ROI on T2 maps. Correlations were excellent for all of the T1 ROIs and the T2* NP ROIs. Highly significant differences in T1 values were found between all Pfirrmann grades except between grades I/II and between grades III/IV. Significant differences were identified in the T2 and the T2* values between all grades except between grades I/III. T1, T2, and T2* relaxation times measurements of the NP are an accurate and time-efficient tool to assess lumbar IDD in sheep. Variable-flip-angle T1 mapping may be further considered as a valuable method to investigate IDD and to assess the efficacy of regenerative treatments in longitudinal studies.https://doi.org/10.1038/s41598-022-09348-w |
| spellingShingle | Nora Bouhsina Cyrille Decante Jean-Baptiste Hardel Dominique Rouleau Jérôme Abadie Antoine Hamel Catherine Le Visage Julie Lesoeur Jérôme Guicheux Johann Clouet Marion Fusellier Comparison of MRI T1, T2, and T2* mapping with histology for assessment of intervertebral disc degeneration in an ovine model Scientific Reports |
| title | Comparison of MRI T1, T2, and T2* mapping with histology for assessment of intervertebral disc degeneration in an ovine model |
| title_full | Comparison of MRI T1, T2, and T2* mapping with histology for assessment of intervertebral disc degeneration in an ovine model |
| title_fullStr | Comparison of MRI T1, T2, and T2* mapping with histology for assessment of intervertebral disc degeneration in an ovine model |
| title_full_unstemmed | Comparison of MRI T1, T2, and T2* mapping with histology for assessment of intervertebral disc degeneration in an ovine model |
| title_short | Comparison of MRI T1, T2, and T2* mapping with histology for assessment of intervertebral disc degeneration in an ovine model |
| title_sort | comparison of mri t1 t2 and t2 mapping with histology for assessment of intervertebral disc degeneration in an ovine model |
| url | https://doi.org/10.1038/s41598-022-09348-w |
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