Histomorphometric effects of Kigelia africana (Bignoniaceae) fruit extract on the testis following short-term treatment with cisplatin in male Sprague–Dawley rats
Objective: To investigate the short-term effects of Kigelia africana fruit extract (KAFE) on cisplatin-induced testicular histo-morphometric changes in Sprague–Dawley (SD) rats. Design: This is an experimental animal study. Materials and methods: Fifty-seven male SD rats were acclamatized and groupe...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2010-07-01
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| Series: | Middle East Fertility Society Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110569010000610 |
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| author | O.O. Azu F.I.O. Duru A.A. Osinubi A.A. Oremosu C.C. Noronha S.O. Elesha A.O. Okanlawon |
| author_facet | O.O. Azu F.I.O. Duru A.A. Osinubi A.A. Oremosu C.C. Noronha S.O. Elesha A.O. Okanlawon |
| author_sort | O.O. Azu |
| collection | DOAJ |
| description | Objective: To investigate the short-term effects of Kigelia africana fruit extract (KAFE) on cisplatin-induced testicular histo-morphometric changes in Sprague–Dawley (SD) rats.
Design: This is an experimental animal study.
Materials and methods: Fifty-seven male SD rats were acclamatized and grouped into 10 of five rats per group. Each rat was administered either KAFE in 100 and 500mg/kg doses orally or cisplatin 10mg/kg i.p. or normal saline to controls. Experiment was terminated after 28days by i.p. injection of ketamin 50mg/kg. Testicular tissue was processed for histological and morphometric analyses while catalase enzyme activity, lipid peroxidation and glutathione levels were assayed accordingly. Sperm count/motility was also assessed.
Results: Cisplatin treatment caused over 37.5% mortality of SD rats. Qualitative histological assessment showed no deleterious changes following treatment with KAFE alone or as a pre-treatment with cisplatin. KAFE post-treatment resulted in focal vacuolar changes in the seminiferous tubules (ST) of the SD rats. Cisplatin-treatment negatively affected the histoarchitecture of the seminiferous tubules with massive loss of spermatogenic cells. There was also a significant reduction in testicular weight/volume, ST diameter and cross-sectional areas (P<0.001) but KAFE positively improved these parameters. KAFE alone and as prophylaxis significantly increased body weight, serum testosterone and follicle-stimulating hormone (P<0.001). It showed a significant elevation in catalase activity, decline in malondialdehyde and up-regulated glutathione levels (P<0.001). These parameters were negatively affected by cisplatin treatment.
Conclusion: The cytoprotection against cisplatin-induced testicular damage by KAFE is likely via an antioxidant modulatory pathway. Also, it is possible that KAFE possesses an androgen-stimulating property. |
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| institution | Directory Open Access Journal |
| issn | 1110-5690 |
| language | English |
| last_indexed | 2024-12-16T13:23:40Z |
| publishDate | 2010-07-01 |
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| series | Middle East Fertility Society Journal |
| spelling | doaj.art-a8db02d86dc844dcbeba4ee799dfd9b52022-12-21T22:30:17ZengSpringerOpenMiddle East Fertility Society Journal1110-56902010-07-0115320020810.1016/j.mefs.2010.07.001Histomorphometric effects of Kigelia africana (Bignoniaceae) fruit extract on the testis following short-term treatment with cisplatin in male Sprague–Dawley ratsO.O. Azu0F.I.O. Duru1A.A. Osinubi2A.A. Oremosu3C.C. Noronha4S.O. Elesha5A.O. Okanlawon6Department of Anatomy, College of Medicine, P.M.B. 12003, Idi-araba, Lagos, NigeriaDepartment of Anatomy, College of Medicine, P.M.B. 12003, Idi-araba, Lagos, NigeriaDepartment of Anatomy, College of Medicine, P.M.B. 12003, Idi-araba, Lagos, NigeriaDepartment of Anatomy, College of Medicine, P.M.B. 12003, Idi-araba, Lagos, NigeriaDepartment of Anatomy, College of Medicine, P.M.B. 12003, Idi-araba, Lagos, NigeriaDepartment of Morbid Anatomy, College of Medicine of the University of Lagos, NigeriaDepartment of Anatomy, College of Medicine, P.M.B. 12003, Idi-araba, Lagos, NigeriaObjective: To investigate the short-term effects of Kigelia africana fruit extract (KAFE) on cisplatin-induced testicular histo-morphometric changes in Sprague–Dawley (SD) rats. Design: This is an experimental animal study. Materials and methods: Fifty-seven male SD rats were acclamatized and grouped into 10 of five rats per group. Each rat was administered either KAFE in 100 and 500mg/kg doses orally or cisplatin 10mg/kg i.p. or normal saline to controls. Experiment was terminated after 28days by i.p. injection of ketamin 50mg/kg. Testicular tissue was processed for histological and morphometric analyses while catalase enzyme activity, lipid peroxidation and glutathione levels were assayed accordingly. Sperm count/motility was also assessed. Results: Cisplatin treatment caused over 37.5% mortality of SD rats. Qualitative histological assessment showed no deleterious changes following treatment with KAFE alone or as a pre-treatment with cisplatin. KAFE post-treatment resulted in focal vacuolar changes in the seminiferous tubules (ST) of the SD rats. Cisplatin-treatment negatively affected the histoarchitecture of the seminiferous tubules with massive loss of spermatogenic cells. There was also a significant reduction in testicular weight/volume, ST diameter and cross-sectional areas (P<0.001) but KAFE positively improved these parameters. KAFE alone and as prophylaxis significantly increased body weight, serum testosterone and follicle-stimulating hormone (P<0.001). It showed a significant elevation in catalase activity, decline in malondialdehyde and up-regulated glutathione levels (P<0.001). These parameters were negatively affected by cisplatin treatment. Conclusion: The cytoprotection against cisplatin-induced testicular damage by KAFE is likely via an antioxidant modulatory pathway. Also, it is possible that KAFE possesses an androgen-stimulating property.http://www.sciencedirect.com/science/article/pii/S1110569010000610Kigelia africanaCisplatinHistologyMorphometryHormone assayLipid peroxidation |
| spellingShingle | O.O. Azu F.I.O. Duru A.A. Osinubi A.A. Oremosu C.C. Noronha S.O. Elesha A.O. Okanlawon Histomorphometric effects of Kigelia africana (Bignoniaceae) fruit extract on the testis following short-term treatment with cisplatin in male Sprague–Dawley rats Middle East Fertility Society Journal Kigelia africana Cisplatin Histology Morphometry Hormone assay Lipid peroxidation |
| title | Histomorphometric effects of Kigelia africana (Bignoniaceae) fruit extract on the testis following short-term treatment with cisplatin in male Sprague–Dawley rats |
| title_full | Histomorphometric effects of Kigelia africana (Bignoniaceae) fruit extract on the testis following short-term treatment with cisplatin in male Sprague–Dawley rats |
| title_fullStr | Histomorphometric effects of Kigelia africana (Bignoniaceae) fruit extract on the testis following short-term treatment with cisplatin in male Sprague–Dawley rats |
| title_full_unstemmed | Histomorphometric effects of Kigelia africana (Bignoniaceae) fruit extract on the testis following short-term treatment with cisplatin in male Sprague–Dawley rats |
| title_short | Histomorphometric effects of Kigelia africana (Bignoniaceae) fruit extract on the testis following short-term treatment with cisplatin in male Sprague–Dawley rats |
| title_sort | histomorphometric effects of kigelia africana bignoniaceae fruit extract on the testis following short term treatment with cisplatin in male sprague dawley rats |
| topic | Kigelia africana Cisplatin Histology Morphometry Hormone assay Lipid peroxidation |
| url | http://www.sciencedirect.com/science/article/pii/S1110569010000610 |
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