Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome c
<p>Abstract</p> <p>Background</p> <p>Oxymatrine, an isolated extract from traditional Chinese herb <it>Sophora Flavescens Ait</it>, has been traditionally used for therapy of anti-hepatitis B virus, anti-inflammation and anti-anaphylaxis. The present study w...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-06-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Online Access: | http://www.jeccr.com/content/30/1/66 |
| _version_ | 1811251491576479744 |
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| author | Wang Bei Zhou Bin Wang Weibing Wei Xuyong Xu Xiao Ling Qi Zheng Shusen |
| author_facet | Wang Bei Zhou Bin Wang Weibing Wei Xuyong Xu Xiao Ling Qi Zheng Shusen |
| author_sort | Wang Bei |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Oxymatrine, an isolated extract from traditional Chinese herb <it>Sophora Flavescens Ait</it>, has been traditionally used for therapy of anti-hepatitis B virus, anti-inflammation and anti-anaphylaxis. The present study was to investigate the anti-cancer effect of oxymatrine on human pancreatic cancer PANC-1 cells, and its possible molecular mechanism.</p> <p>Methods</p> <p>The effect of oxymatrine on the viability and apoptosis was examined by methyl thiazolyl tetrazolium and flow cytometry analysis. The expression of Bax, Bcl-2, Bcl-x (L/S), Bid, Bad, HIAP-1, HIAP-2, XIAP, NAIP, Livin and Survivin genes was accessed by RT-PCR. The levels of cytochrome c and caspase 3 protein were assessed by Western blotting.</p> <p>Results</p> <p>Oxymatrine inhibited cell viability and induced apoptosis of PANC-1 cells in a time- and dose-dependent manner. This was accompanied by down-regulated expression of Livin and Survivin genes while the Bax/Bcl-2 ratio was upregulated. Furthermore, oxymatrine treatment led to the release of cytochrome c and activation of caspase-3 proteins.</p> <p>Conclusion</p> <p>Oxymatrine can induce apoptotic cell death of human pancreatic cancer, which might be attributed to the regulation of Bcl-2 and IAP families, release of mitochondrial cytochrome c and activation of caspase-3.</p> |
| first_indexed | 2024-04-12T16:21:15Z |
| format | Article |
| id | doaj.art-a8db0d2f25d4463ea87222e18783286b |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-04-12T16:21:15Z |
| publishDate | 2011-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-a8db0d2f25d4463ea87222e18783286b2022-12-22T03:25:34ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662011-06-013016610.1186/1756-9966-30-66Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome cWang BeiZhou BinWang WeibingWei XuyongXu XiaoLing QiZheng Shusen<p>Abstract</p> <p>Background</p> <p>Oxymatrine, an isolated extract from traditional Chinese herb <it>Sophora Flavescens Ait</it>, has been traditionally used for therapy of anti-hepatitis B virus, anti-inflammation and anti-anaphylaxis. The present study was to investigate the anti-cancer effect of oxymatrine on human pancreatic cancer PANC-1 cells, and its possible molecular mechanism.</p> <p>Methods</p> <p>The effect of oxymatrine on the viability and apoptosis was examined by methyl thiazolyl tetrazolium and flow cytometry analysis. The expression of Bax, Bcl-2, Bcl-x (L/S), Bid, Bad, HIAP-1, HIAP-2, XIAP, NAIP, Livin and Survivin genes was accessed by RT-PCR. The levels of cytochrome c and caspase 3 protein were assessed by Western blotting.</p> <p>Results</p> <p>Oxymatrine inhibited cell viability and induced apoptosis of PANC-1 cells in a time- and dose-dependent manner. This was accompanied by down-regulated expression of Livin and Survivin genes while the Bax/Bcl-2 ratio was upregulated. Furthermore, oxymatrine treatment led to the release of cytochrome c and activation of caspase-3 proteins.</p> <p>Conclusion</p> <p>Oxymatrine can induce apoptotic cell death of human pancreatic cancer, which might be attributed to the regulation of Bcl-2 and IAP families, release of mitochondrial cytochrome c and activation of caspase-3.</p>http://www.jeccr.com/content/30/1/66 |
| spellingShingle | Wang Bei Zhou Bin Wang Weibing Wei Xuyong Xu Xiao Ling Qi Zheng Shusen Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome c Journal of Experimental & Clinical Cancer Research |
| title | Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome c |
| title_full | Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome c |
| title_fullStr | Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome c |
| title_full_unstemmed | Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome c |
| title_short | Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome c |
| title_sort | oxymatrine induces human pancreatic cancer panc 1 cells apoptosis via regulating expression of bcl 2 and iap families and releasing of cytochrome c |
| url | http://www.jeccr.com/content/30/1/66 |
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