Species-specific regulatory pathways of small RNAs play sophisticated roles in flower development in Dimocarpus longan Lour.
Flower development plays vital role in horticultural plants. Post-transcriptional regulation via small RNAs is important for plant flower development. To uncover post-transcriptional regulatory networks during the flower development in Dimocarpus longan Lour. ‘Shixia’, an economically important frui...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-04-01
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| Series: | Horticultural Plant Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S246801412200142X |
| _version_ | 1827266836923678720 |
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| author | Bo Liu Guanliang Li Chengjie Chen Zaohai Zeng Jing Xu Jisen Zhang Rui Xia Yuanlong Liu |
| author_facet | Bo Liu Guanliang Li Chengjie Chen Zaohai Zeng Jing Xu Jisen Zhang Rui Xia Yuanlong Liu |
| author_sort | Bo Liu |
| collection | DOAJ |
| description | Flower development plays vital role in horticultural plants. Post-transcriptional regulation via small RNAs is important for plant flower development. To uncover post-transcriptional regulatory networks during the flower development in Dimocarpus longan Lour. ‘Shixia’, an economically important fruit crop in subtropical regions, we collected and analyzed sRNA deep-sequencing datasets and degradome libraries Apart from identifying miRNAs and phased siRNA generating loci (PHAS loci), 120 hairpin loci, producing abundant sRNAs, were identified by in-house protocols. Our results suggested that 56 miRNA-target pairs, 22 21-nt-PHAS loci, and 111 hairpin loci are involved in post-transcriptional gene silencing during longan reproductive development. Lineage-specific or species-specific post-transcriptional regulatory modules have been unveiled, including miR482-PHAS and miRN15. miR482-PHAS might be involved in longan flower development beyond their conserved roles in plant defense, and miRN15 is a novel miRNA likely associated with a hairpin locus (HPL-056) to regulate strigolactone receptor gene DWARF14 (D14) and the biogenesis of phasiRNAs from D14. These small RNAs are enriched in flower buds, suggesting they are likely involved in post-transcriptional regulatory networks essential for longan flower development via the strigolactone signaling pathway. |
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| issn | 2468-0141 |
| language | English |
| last_indexed | 2025-03-22T04:24:11Z |
| publishDate | 2023-04-01 |
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| series | Horticultural Plant Journal |
| spelling | doaj.art-a8db1b8869a44398a84827053a9034a72024-04-28T06:21:55ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412023-04-0192237249Species-specific regulatory pathways of small RNAs play sophisticated roles in flower development in Dimocarpus longan Lour.Bo Liu0Guanliang Li1Chengjie Chen2Zaohai Zeng3Jing Xu4Jisen Zhang5Rui Xia6Yuanlong Liu7State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong 510640, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510640, China; Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510640, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong 510640, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510640, China; Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510640, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong 510640, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510640, China; Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510640, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong 510640, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510640, China; Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510640, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong 510640, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510640, China; Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510640, ChinaCenter for Genomics and Biotechnology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong 510640, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510640, China; Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510640, China; Corresponding authors.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong 510640, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510640, China; Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510640, China; Corresponding authors.Flower development plays vital role in horticultural plants. Post-transcriptional regulation via small RNAs is important for plant flower development. To uncover post-transcriptional regulatory networks during the flower development in Dimocarpus longan Lour. ‘Shixia’, an economically important fruit crop in subtropical regions, we collected and analyzed sRNA deep-sequencing datasets and degradome libraries Apart from identifying miRNAs and phased siRNA generating loci (PHAS loci), 120 hairpin loci, producing abundant sRNAs, were identified by in-house protocols. Our results suggested that 56 miRNA-target pairs, 22 21-nt-PHAS loci, and 111 hairpin loci are involved in post-transcriptional gene silencing during longan reproductive development. Lineage-specific or species-specific post-transcriptional regulatory modules have been unveiled, including miR482-PHAS and miRN15. miR482-PHAS might be involved in longan flower development beyond their conserved roles in plant defense, and miRN15 is a novel miRNA likely associated with a hairpin locus (HPL-056) to regulate strigolactone receptor gene DWARF14 (D14) and the biogenesis of phasiRNAs from D14. These small RNAs are enriched in flower buds, suggesting they are likely involved in post-transcriptional regulatory networks essential for longan flower development via the strigolactone signaling pathway.http://www.sciencedirect.com/science/article/pii/S246801412200142XDimocarpus longan Lour.Small RNAsFlower developmentPost-transcriptional regulation |
| spellingShingle | Bo Liu Guanliang Li Chengjie Chen Zaohai Zeng Jing Xu Jisen Zhang Rui Xia Yuanlong Liu Species-specific regulatory pathways of small RNAs play sophisticated roles in flower development in Dimocarpus longan Lour. Horticultural Plant Journal Dimocarpus longan Lour. Small RNAs Flower development Post-transcriptional regulation |
| title | Species-specific regulatory pathways of small RNAs play sophisticated roles in flower development in Dimocarpus longan Lour. |
| title_full | Species-specific regulatory pathways of small RNAs play sophisticated roles in flower development in Dimocarpus longan Lour. |
| title_fullStr | Species-specific regulatory pathways of small RNAs play sophisticated roles in flower development in Dimocarpus longan Lour. |
| title_full_unstemmed | Species-specific regulatory pathways of small RNAs play sophisticated roles in flower development in Dimocarpus longan Lour. |
| title_short | Species-specific regulatory pathways of small RNAs play sophisticated roles in flower development in Dimocarpus longan Lour. |
| title_sort | species specific regulatory pathways of small rnas play sophisticated roles in flower development in dimocarpus longan lour |
| topic | Dimocarpus longan Lour. Small RNAs Flower development Post-transcriptional regulation |
| url | http://www.sciencedirect.com/science/article/pii/S246801412200142X |
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