Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute Pancreatitis
Jing Wang, Changqin Xu, Hongwei Xu, Ruixia Wang, Tong Su, Shulei Zhao Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, People’s Republic of ChinaCorrespondence: Shulei Zhao, Department of Gastroenterology, Shandong Provincia...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-12-01
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| Series: | Journal of Inflammation Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/single-cell-rna-sequencing-reveals-roles-of-fibroblasts-during-intesti-peer-reviewed-fulltext-article-JIR |
| _version_ | 1797391165851435008 |
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| author | Wang J Xu C Xu H Wang R Su T Zhao S |
| author_facet | Wang J Xu C Xu H Wang R Su T Zhao S |
| author_sort | Wang J |
| collection | DOAJ |
| description | Jing Wang, Changqin Xu, Hongwei Xu, Ruixia Wang, Tong Su, Shulei Zhao Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, People’s Republic of ChinaCorrespondence: Shulei Zhao, Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, 324 Jingwu Weiqi Road, Jinan, 250021, People’s Republic of China, Email wenzhu24@126.comPurpose: To explore the molecular mechanisms of intestinal injury and treatment by analyzing changes in cellular heterogeneity and composition in rat ileal tissue during injury and treatment processes.Methods: We constructed a rat model of SAP and evaluated treatment with an injected of monoacylglycerol lipase (MAGL) inhibitor (JZL184) solution using three experimental groups: healthy male Sprague-Dawley (SD) rats injected with vehicle (CON), male SD SAP model rats injected with vehicle (SAP), and male SAP rats injected with JZL184. We obtained and prepared a single-cell suspension of ileal tissue of each rat for single-cell transcriptome sequencing.Results: This project classified changes in cellular heterogeneity and composition in rat ileal tissue during SAP-induced intestinal injury and MAGL treatment. We found that the number of fibroblast clusters was decreased in the SAP group relative to the CON group, and increased after JZL184 treatment. Further analysis of differences in gene expression between cell clusters in each group reveals that fibroblasts had the greatest number of differentially expressed genes. Most notably, expression of genes involved in communication between cells was found to vary during SAP-induced intestinal injury and JZL184 treatment. Among these changes, the degree of difference in expression of genes involved in communication between fibroblasts and other cells was the highest, indicating that fibroblasts in rat ileal tissue affect intestinal injury and repair through cell-to-cell communication. In addition, our results reveal that differentially expressed RNA-binding proteins in fibroblasts may affect their functions in intestinal injury and treatment by affecting the expression of genes regulating communication between cells.Conclusion: These findings emphasize the importance of understanding the interactions between fibroblasts and other cells in the context of intestinal injury, providing valuable insights for further exploring molecular mechanisms and insight for discovering new treatment targets and strategies.Keywords: single-cell RNA sequencing, cell heterogeneity, fibroblasts, monoacylglycerol lipase, intestinal injury, RNA-binding protein, severe acute pancreatitis |
| first_indexed | 2024-03-08T23:29:06Z |
| format | Article |
| id | doaj.art-a8dc18dae9fc4b34ad3cc50031104e85 |
| institution | Directory Open Access Journal |
| issn | 1178-7031 |
| language | English |
| last_indexed | 2024-03-08T23:29:06Z |
| publishDate | 2023-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj.art-a8dc18dae9fc4b34ad3cc50031104e852023-12-14T17:01:45ZengDove Medical PressJournal of Inflammation Research1178-70312023-12-01Volume 166073608688966Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute PancreatitisWang JXu CXu HWang RSu TZhao SJing Wang, Changqin Xu, Hongwei Xu, Ruixia Wang, Tong Su, Shulei Zhao Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, People’s Republic of ChinaCorrespondence: Shulei Zhao, Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, 324 Jingwu Weiqi Road, Jinan, 250021, People’s Republic of China, Email wenzhu24@126.comPurpose: To explore the molecular mechanisms of intestinal injury and treatment by analyzing changes in cellular heterogeneity and composition in rat ileal tissue during injury and treatment processes.Methods: We constructed a rat model of SAP and evaluated treatment with an injected of monoacylglycerol lipase (MAGL) inhibitor (JZL184) solution using three experimental groups: healthy male Sprague-Dawley (SD) rats injected with vehicle (CON), male SD SAP model rats injected with vehicle (SAP), and male SAP rats injected with JZL184. We obtained and prepared a single-cell suspension of ileal tissue of each rat for single-cell transcriptome sequencing.Results: This project classified changes in cellular heterogeneity and composition in rat ileal tissue during SAP-induced intestinal injury and MAGL treatment. We found that the number of fibroblast clusters was decreased in the SAP group relative to the CON group, and increased after JZL184 treatment. Further analysis of differences in gene expression between cell clusters in each group reveals that fibroblasts had the greatest number of differentially expressed genes. Most notably, expression of genes involved in communication between cells was found to vary during SAP-induced intestinal injury and JZL184 treatment. Among these changes, the degree of difference in expression of genes involved in communication between fibroblasts and other cells was the highest, indicating that fibroblasts in rat ileal tissue affect intestinal injury and repair through cell-to-cell communication. In addition, our results reveal that differentially expressed RNA-binding proteins in fibroblasts may affect their functions in intestinal injury and treatment by affecting the expression of genes regulating communication between cells.Conclusion: These findings emphasize the importance of understanding the interactions between fibroblasts and other cells in the context of intestinal injury, providing valuable insights for further exploring molecular mechanisms and insight for discovering new treatment targets and strategies.Keywords: single-cell RNA sequencing, cell heterogeneity, fibroblasts, monoacylglycerol lipase, intestinal injury, RNA-binding protein, severe acute pancreatitishttps://www.dovepress.com/single-cell-rna-sequencing-reveals-roles-of-fibroblasts-during-intesti-peer-reviewed-fulltext-article-JIRsingle-cell rna sequencingcell heterogeneityfibroblastsmonoacylglycerol lipaseintestinal injuryrna-binding proteinsevere acute pancreatitis. |
| spellingShingle | Wang J Xu C Xu H Wang R Su T Zhao S Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute Pancreatitis Journal of Inflammation Research single-cell rna sequencing cell heterogeneity fibroblasts monoacylglycerol lipase intestinal injury rna-binding protein severe acute pancreatitis. |
| title | Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute Pancreatitis |
| title_full | Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute Pancreatitis |
| title_fullStr | Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute Pancreatitis |
| title_full_unstemmed | Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute Pancreatitis |
| title_short | Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute Pancreatitis |
| title_sort | single cell rna sequencing reveals roles of fibroblasts during intestinal injury and repair in rats with severe acute pancreatitis |
| topic | single-cell rna sequencing cell heterogeneity fibroblasts monoacylglycerol lipase intestinal injury rna-binding protein severe acute pancreatitis. |
| url | https://www.dovepress.com/single-cell-rna-sequencing-reveals-roles-of-fibroblasts-during-intesti-peer-reviewed-fulltext-article-JIR |
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