Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity
Objective: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. Materials and Met...
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| Format: | Article |
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Mashhad University of Medical Sciences
2016-04-01
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| Series: | Avicenna Journal of Phytomedicine |
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| Online Access: | http://ajp.mums.ac.ir/article_5962_f3d10d3d831ba187f1f82268f4e3a7fd.pdf |
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| author | Soheila Sepahi Abbas Jafarian-Dehkordi Maryam Mirlohi kobra shirani Mahmoud Etebari |
| author_facet | Soheila Sepahi Abbas Jafarian-Dehkordi Maryam Mirlohi kobra shirani Mahmoud Etebari |
| author_sort | Soheila Sepahi |
| collection | DOAJ |
| description | Objective: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. Materials and Methods: HepG2 were incubated with irinotecan (100 µM), heat-killed cells (0.025 µg/ml) + irinotecan (100 µM), and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum A7 + irinotecan (100 µM). Phosphate buffered saline (PBS) was used as negative control. Results: Irinotecan was shown to induce DNA damage in HepG2 cells. The results showed that heat-killed cells (0.025 µg/ml) and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum significantly reduce irinotecan- induced DNA damage. Conclusion: Our results indicate that L. plantarum A7 can decrease the genotoxic effects of irinotecan in HepG2 cells, in vitro. This finding may be supportive for the optimization of therapeutic efficacy in irinotecan treatment. |
| first_indexed | 2024-12-12T16:50:52Z |
| format | Article |
| id | doaj.art-a8dc4152412942fa87fd909b3ba50dc5 |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-12-12T16:50:52Z |
| publishDate | 2016-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-a8dc4152412942fa87fd909b3ba50dc52022-12-22T00:18:23ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492016-04-01633293355962Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicitySoheila Sepahi0Abbas Jafarian-Dehkordi1Maryam Mirlohi2kobra shirani3Mahmoud Etebari4Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.Food Security Research Centre, School of Nutrition and Food Sciences, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.Objective: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. Materials and Methods: HepG2 were incubated with irinotecan (100 µM), heat-killed cells (0.025 µg/ml) + irinotecan (100 µM), and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum A7 + irinotecan (100 µM). Phosphate buffered saline (PBS) was used as negative control. Results: Irinotecan was shown to induce DNA damage in HepG2 cells. The results showed that heat-killed cells (0.025 µg/ml) and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum significantly reduce irinotecan- induced DNA damage. Conclusion: Our results indicate that L. plantarum A7 can decrease the genotoxic effects of irinotecan in HepG2 cells, in vitro. This finding may be supportive for the optimization of therapeutic efficacy in irinotecan treatment.http://ajp.mums.ac.ir/article_5962_f3d10d3d831ba187f1f82268f4e3a7fd.pdfIrinotecanLactobacillus plantarum A7Anti-genotoxicityHepG2Comet assay |
| spellingShingle | Soheila Sepahi Abbas Jafarian-Dehkordi Maryam Mirlohi kobra shirani Mahmoud Etebari Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity Avicenna Journal of Phytomedicine Irinotecan Lactobacillus plantarum A7 Anti-genotoxicity HepG2 Comet assay |
| title | Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity |
| title_full | Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity |
| title_fullStr | Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity |
| title_full_unstemmed | Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity |
| title_short | Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity |
| title_sort | protective role of lactobacillus plantarum a7 against irinotecan induced genotoxicity |
| topic | Irinotecan Lactobacillus plantarum A7 Anti-genotoxicity HepG2 Comet assay |
| url | http://ajp.mums.ac.ir/article_5962_f3d10d3d831ba187f1f82268f4e3a7fd.pdf |
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