<i>Arthrospira platensis</i> Mutagenesis for Protein and C-Phycocyanin Improvement and Proteomics Approaches
<i>Arthrospira (Spirulina) platensis</i> is known for its use as a food supplement, with reported therapeutic properties including antiviral, anti-inflammatory and antioxidant activity. <i>Arthrospira</i> is also an excellent source of proteins and C-phycocyanin. The latter i...
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MDPI AG
2022-06-01
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| Online Access: | https://www.mdpi.com/2075-1729/12/6/911 |
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| author | Wanida Pan-utai Siriluck Iamtham Sittiruk Roytrakul Sarn Settachaimongkon Ladda Sangduean Wattanasiritham Sumitra Boonbumrung Juta Mookdasanit Sayamon Sithtisarn |
| author_facet | Wanida Pan-utai Siriluck Iamtham Sittiruk Roytrakul Sarn Settachaimongkon Ladda Sangduean Wattanasiritham Sumitra Boonbumrung Juta Mookdasanit Sayamon Sithtisarn |
| author_sort | Wanida Pan-utai |
| collection | DOAJ |
| description | <i>Arthrospira (Spirulina) platensis</i> is known for its use as a food supplement, with reported therapeutic properties including antiviral, anti-inflammatory and antioxidant activity. <i>Arthrospira</i> is also an excellent source of proteins and C-phycocyanin. The latter is a light-harvesting pigment-protein complex in cyanobacteria, located on the outer surface of the thylakoid membrane and comprising 40 to 60% of the total soluble protein in cells. Random mutagenesis is a useful tool as a non-genetically modified mutation method that has been widely used to generate mutants of different microorganisms. Exposure of microalgae or cyanobacteria to chemical stimuli affects their growth and many biological processes. Chemicals influence several proteins, including those involved in carbohydrate and energy metabolisms, photosynthesis and stress-related proteins (oxidative stress-reactive oxygen species (ROS) scavenging enzymes). Signal transduction pathways and ion transportation mechanisms are also impacted by chemical treatment, with changes causing the production of numerous biomolecules and stimulation of defence responses. This study compared the protein contents of <i>A. platensis</i> control and after mutagenesis using diethyl sulphate (DES) under various treatment concentrations for effective mutation of <i>A. platensis.</i> Results identified 1152 peptides using proteomics approaches. The proteins were classified into 23 functional categories. Random mutagenesis of <i>A. platensis</i> by DES was found to be highly effective for C-phycocyanin and protein production. |
| first_indexed | 2024-03-09T23:14:55Z |
| format | Article |
| id | doaj.art-a8de27abcdcc4acd844c008798a4bfad |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T23:14:55Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-a8de27abcdcc4acd844c008798a4bfad2023-11-23T17:37:38ZengMDPI AGLife2075-17292022-06-0112691110.3390/life12060911<i>Arthrospira platensis</i> Mutagenesis for Protein and C-Phycocyanin Improvement and Proteomics ApproachesWanida Pan-utai0Siriluck Iamtham1Sittiruk Roytrakul2Sarn Settachaimongkon3Ladda Sangduean Wattanasiritham4Sumitra Boonbumrung5Juta Mookdasanit6Sayamon Sithtisarn7Department of Applied Microbiology, Institute of Food Research and Product Development, Kasetsart University, Chatuchak, Bangkok 10900, ThailandDepartment of Science, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, ThailandFunctional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Rd., Pathum Thani 12120, ThailandDepartment of Food Technology, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok 10330, ThailandDepartment of Food Chemistry and Physics, Institute of Food Research and Product Development, Kasetsart University, Chatuchak, Bangkok 10900, ThailandDepartment of Food Chemistry and Physics, Institute of Food Research and Product Development, Kasetsart University, Chatuchak, Bangkok 10900, ThailandDepartment of Fishery Products, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok 10900, ThailandDepartment of Applied Microbiology, Institute of Food Research and Product Development, Kasetsart University, Chatuchak, Bangkok 10900, Thailand<i>Arthrospira (Spirulina) platensis</i> is known for its use as a food supplement, with reported therapeutic properties including antiviral, anti-inflammatory and antioxidant activity. <i>Arthrospira</i> is also an excellent source of proteins and C-phycocyanin. The latter is a light-harvesting pigment-protein complex in cyanobacteria, located on the outer surface of the thylakoid membrane and comprising 40 to 60% of the total soluble protein in cells. Random mutagenesis is a useful tool as a non-genetically modified mutation method that has been widely used to generate mutants of different microorganisms. Exposure of microalgae or cyanobacteria to chemical stimuli affects their growth and many biological processes. Chemicals influence several proteins, including those involved in carbohydrate and energy metabolisms, photosynthesis and stress-related proteins (oxidative stress-reactive oxygen species (ROS) scavenging enzymes). Signal transduction pathways and ion transportation mechanisms are also impacted by chemical treatment, with changes causing the production of numerous biomolecules and stimulation of defence responses. This study compared the protein contents of <i>A. platensis</i> control and after mutagenesis using diethyl sulphate (DES) under various treatment concentrations for effective mutation of <i>A. platensis.</i> Results identified 1152 peptides using proteomics approaches. The proteins were classified into 23 functional categories. Random mutagenesis of <i>A. platensis</i> by DES was found to be highly effective for C-phycocyanin and protein production.https://www.mdpi.com/2075-1729/12/6/911<i>Arthrospira</i>C-phycocyaninmutagenesisproteinproteomics |
| spellingShingle | Wanida Pan-utai Siriluck Iamtham Sittiruk Roytrakul Sarn Settachaimongkon Ladda Sangduean Wattanasiritham Sumitra Boonbumrung Juta Mookdasanit Sayamon Sithtisarn <i>Arthrospira platensis</i> Mutagenesis for Protein and C-Phycocyanin Improvement and Proteomics Approaches Life <i>Arthrospira</i> C-phycocyanin mutagenesis protein proteomics |
| title | <i>Arthrospira platensis</i> Mutagenesis for Protein and C-Phycocyanin Improvement and Proteomics Approaches |
| title_full | <i>Arthrospira platensis</i> Mutagenesis for Protein and C-Phycocyanin Improvement and Proteomics Approaches |
| title_fullStr | <i>Arthrospira platensis</i> Mutagenesis for Protein and C-Phycocyanin Improvement and Proteomics Approaches |
| title_full_unstemmed | <i>Arthrospira platensis</i> Mutagenesis for Protein and C-Phycocyanin Improvement and Proteomics Approaches |
| title_short | <i>Arthrospira platensis</i> Mutagenesis for Protein and C-Phycocyanin Improvement and Proteomics Approaches |
| title_sort | i arthrospira platensis i mutagenesis for protein and c phycocyanin improvement and proteomics approaches |
| topic | <i>Arthrospira</i> C-phycocyanin mutagenesis protein proteomics |
| url | https://www.mdpi.com/2075-1729/12/6/911 |
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