Modulation of the Functional State of Mouse Neutrophils by Selenium Nanoparticles In Vivo
This study aimed to discover the immunomodulatory effect of selenium nanoparticles (SeNPs) on the functional state of neutrophils in vivo. Intraperitoneal injections of SeNPs (size 100 nm) 2.5 mg/kg/daily to BALB/c mice for a duration of 7–28 days led to the development of an inflammatory reaction,...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/13651 |
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| author | Valentina N. Mal’tseva Sergey V. Gudkov Egor A. Turovsky |
| author_facet | Valentina N. Mal’tseva Sergey V. Gudkov Egor A. Turovsky |
| author_sort | Valentina N. Mal’tseva |
| collection | DOAJ |
| description | This study aimed to discover the immunomodulatory effect of selenium nanoparticles (SeNPs) on the functional state of neutrophils in vivo. Intraperitoneal injections of SeNPs (size 100 nm) 2.5 mg/kg/daily to BALB/c mice for a duration of 7–28 days led to the development of an inflammatory reaction, which was registered by a significant increase in the number of neutrophils released from the peritoneal cavity, as well as their activated state, without additional effects. At the same time, subcutaneous injections of the same SeNPs preparations at concentrations of 0.1, 0.5, and 2.5 mg/kg, on the contrary, modulated the functional state of neutrophils depending on the concentration and duration of SeNPs administration. With the use of fluorescence spectroscopy, chemiluminescence, biochemical methods, and PCR analysis, it was found that subcutaneous administration of SeNPs (0.1, 0.5, and 2.5 mg/kg) to mice for a short period of time (7–14 days) leads to modification of important neutrophil functions (adhesion, the number of migrating cells into the peritoneal cell cavity, ROS production, and NET formation). The obtained results indicated the immunostimulatory and antioxidant effects of SeNPs in vivo during short-term administration, while the most pronounced immunomodulatory effects of SeNPs were observed with the introduction of a low concentration of SeNPs (0.1 mg/kg). Increase in the administration time of SeNPs (0.1 mg/kg or 2.5 mg/kg) up to 28 days led to a decrease in the adhesive abilities of neutrophils and suppression of the expression of mRNA of adhesive molecules, as well as proteins involved in the generation of ROS, with the exception of NOX2; there was a tendency to suppress gene expression pro-inflammatory factors, which indicates the possible manifestation of immunosuppressive and anti-inflammatory effects of SeNPs during their long-term administration. Changes in the expression of selenoproteins also had features depending on the concentration and duration of the administered SeNPs. Selenoprotein P, selenoprotein M, selenoprotein S, selenoprotein K, and selenoprotein T were the most sensitive to the introduction of SeNPs into the mouse organism, which indicates their participation in maintaining the functional status of neutrophils, and possibly mediated the immunomodulatory effect of SeNPs. |
| first_indexed | 2024-03-09T18:58:50Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:58:50Z |
| publishDate | 2022-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a8de5d78e6a045878f9a625eb046db9a2023-11-24T05:11:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123211365110.3390/ijms232113651Modulation of the Functional State of Mouse Neutrophils by Selenium Nanoparticles In VivoValentina N. Mal’tseva0Sergey V. Gudkov1Egor A. Turovsky2Institute of Cell Biophysics of the Russian Academy of Sciences, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290 Pushchino, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilove St., 119991 Moscow, RussiaInstitute of Cell Biophysics of the Russian Academy of Sciences, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290 Pushchino, RussiaThis study aimed to discover the immunomodulatory effect of selenium nanoparticles (SeNPs) on the functional state of neutrophils in vivo. Intraperitoneal injections of SeNPs (size 100 nm) 2.5 mg/kg/daily to BALB/c mice for a duration of 7–28 days led to the development of an inflammatory reaction, which was registered by a significant increase in the number of neutrophils released from the peritoneal cavity, as well as their activated state, without additional effects. At the same time, subcutaneous injections of the same SeNPs preparations at concentrations of 0.1, 0.5, and 2.5 mg/kg, on the contrary, modulated the functional state of neutrophils depending on the concentration and duration of SeNPs administration. With the use of fluorescence spectroscopy, chemiluminescence, biochemical methods, and PCR analysis, it was found that subcutaneous administration of SeNPs (0.1, 0.5, and 2.5 mg/kg) to mice for a short period of time (7–14 days) leads to modification of important neutrophil functions (adhesion, the number of migrating cells into the peritoneal cell cavity, ROS production, and NET formation). The obtained results indicated the immunostimulatory and antioxidant effects of SeNPs in vivo during short-term administration, while the most pronounced immunomodulatory effects of SeNPs were observed with the introduction of a low concentration of SeNPs (0.1 mg/kg). Increase in the administration time of SeNPs (0.1 mg/kg or 2.5 mg/kg) up to 28 days led to a decrease in the adhesive abilities of neutrophils and suppression of the expression of mRNA of adhesive molecules, as well as proteins involved in the generation of ROS, with the exception of NOX2; there was a tendency to suppress gene expression pro-inflammatory factors, which indicates the possible manifestation of immunosuppressive and anti-inflammatory effects of SeNPs during their long-term administration. Changes in the expression of selenoproteins also had features depending on the concentration and duration of the administered SeNPs. Selenoprotein P, selenoprotein M, selenoprotein S, selenoprotein K, and selenoprotein T were the most sensitive to the introduction of SeNPs into the mouse organism, which indicates their participation in maintaining the functional status of neutrophils, and possibly mediated the immunomodulatory effect of SeNPs.https://www.mdpi.com/1422-0067/23/21/13651neutrophilsselenium nanoparticlesROS productionadhesionNETsgenes |
| spellingShingle | Valentina N. Mal’tseva Sergey V. Gudkov Egor A. Turovsky Modulation of the Functional State of Mouse Neutrophils by Selenium Nanoparticles In Vivo International Journal of Molecular Sciences neutrophils selenium nanoparticles ROS production adhesion NETs genes |
| title | Modulation of the Functional State of Mouse Neutrophils by Selenium Nanoparticles In Vivo |
| title_full | Modulation of the Functional State of Mouse Neutrophils by Selenium Nanoparticles In Vivo |
| title_fullStr | Modulation of the Functional State of Mouse Neutrophils by Selenium Nanoparticles In Vivo |
| title_full_unstemmed | Modulation of the Functional State of Mouse Neutrophils by Selenium Nanoparticles In Vivo |
| title_short | Modulation of the Functional State of Mouse Neutrophils by Selenium Nanoparticles In Vivo |
| title_sort | modulation of the functional state of mouse neutrophils by selenium nanoparticles in vivo |
| topic | neutrophils selenium nanoparticles ROS production adhesion NETs genes |
| url | https://www.mdpi.com/1422-0067/23/21/13651 |
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