Identification of a novel locus C2 controlling canary yellow flesh color in watermelons
The flesh color of watermelon is an important trait that is determined by carotenoid composition and affects consumers’ fruit desirability. Although a complete dominant control by C locus (Cllcyb) for canary yellow flesh (CY) over red flesh has been reported, red and CY colors frequently appear as a...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1256627/full |
| _version_ | 1797681389556989952 |
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| author | Girim Park Durre Shahwar Gaeun Jang Jagyeong Shin Gibeom Kwon Younjae Kim Chang Oh Hong Chang Oh Hong Bingkui Jin Hoytaek Kim Oakjin Lee Younghoon Park Younghoon Park |
| author_facet | Girim Park Durre Shahwar Gaeun Jang Jagyeong Shin Gibeom Kwon Younjae Kim Chang Oh Hong Chang Oh Hong Bingkui Jin Hoytaek Kim Oakjin Lee Younghoon Park Younghoon Park |
| author_sort | Girim Park |
| collection | DOAJ |
| description | The flesh color of watermelon is an important trait that is determined by carotenoid composition and affects consumers’ fruit desirability. Although a complete dominant control by C locus (Cllcyb) for canary yellow flesh (CY) over red flesh has been reported, red and CY colors frequently appear as a mixed pattern in the same flesh (incomplete canary yellow, ICY) in F1 and inbred lines carrying dominant C alleles. Therefore, we examined the genetic control of the mixed color pattern in ICY using whole-genome resequencing of three ICY (ICY group) and three CY inbred lines (CY group), as well as genetic linkage mapping of an F2 population. The segregation pattern in 135 F2 plants indicated that CY is controlled by a single locus (named C2) dominant over ICY. The whole-genome resequencing of ICY and CY inbred lines revealed an ICY/CY-specific region of approximately 27.60–27.88 Mb on Chr. 2 that was polymorphic between the ICY and CY groups. Our genetic map, using nine cleaved amplified polymorphic sequence markers developed based on the single-nucleotide polymorphisms from the ICY/CY-specific region, confirmed that C2 is located on Chr. 2 and cosegregated with the marker (M7) derived from a non-synonymous single-nucleotide polymorphism of the pentatricopeptide repeat (PPR) gene (ClPPR, Cla97C02G039880). Additionally, 27 watermelon inbred lines of ICY, CY, and red flesh were evaluated using previously reported Cllcyb (C locus)-based markers and our C2 locus-linked ClPPR-based marker (M7). As a result, dominant alleles at the C2 locus were required to produce CY, in addition to dominant alleles at the C locus, while a recessive homozygous genotype at the C locus gave the red flesh irrespective of the genotype at the C2 locus. Using a ClPPR-based cleaved amplified polymorphic sequence developed in this study and Cllcyb-based markers, watermelon cultivars with CY, ICY, and red flesh could be successfully discerned, implying that the combined use of these markers will be efficient for marker-assisted selection of flesh color in watermelon breeding. |
| first_indexed | 2024-03-11T23:44:14Z |
| format | Article |
| id | doaj.art-a8debc4f494a45f6a784857f7944726f |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-03-11T23:44:14Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-a8debc4f494a45f6a784857f7944726f2023-09-19T15:07:00ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-09-011410.3389/fgene.2023.12566271256627Identification of a novel locus C2 controlling canary yellow flesh color in watermelonsGirim Park0Durre Shahwar1Gaeun Jang2Jagyeong Shin3Gibeom Kwon4Younjae Kim5Chang Oh Hong6Chang Oh Hong7Bingkui Jin8Hoytaek Kim9Oakjin Lee10Younghoon Park11Younghoon Park12Department of Horticultural Bioscience, Pusan National University, Miryang, Republic of KoreaDepartment of Horticultural Bioscience, Pusan National University, Miryang, Republic of KoreaDepartment of Horticultural Bioscience, Pusan National University, Miryang, Republic of KoreaDepartment of Horticultural Bioscience, Pusan National University, Miryang, Republic of KoreaPartner Seeds Co., Ltd., Gimje, Republic of KoreaPartner Seeds Co., Ltd., Gimje, Republic of KoreaDepartment of Life Science and Environmental Biochemistry, Pusan National University, Miryang, Republic of KoreaLife and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of KoreaUNELL Biotechnology Co., Ltd., Weifang, ChinaDepartment of Horticulture, Sunchon National University, Sunchon, Republic of KoreaNational Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju, Republic of KoreaDepartment of Horticultural Bioscience, Pusan National University, Miryang, Republic of KoreaLife and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of KoreaThe flesh color of watermelon is an important trait that is determined by carotenoid composition and affects consumers’ fruit desirability. Although a complete dominant control by C locus (Cllcyb) for canary yellow flesh (CY) over red flesh has been reported, red and CY colors frequently appear as a mixed pattern in the same flesh (incomplete canary yellow, ICY) in F1 and inbred lines carrying dominant C alleles. Therefore, we examined the genetic control of the mixed color pattern in ICY using whole-genome resequencing of three ICY (ICY group) and three CY inbred lines (CY group), as well as genetic linkage mapping of an F2 population. The segregation pattern in 135 F2 plants indicated that CY is controlled by a single locus (named C2) dominant over ICY. The whole-genome resequencing of ICY and CY inbred lines revealed an ICY/CY-specific region of approximately 27.60–27.88 Mb on Chr. 2 that was polymorphic between the ICY and CY groups. Our genetic map, using nine cleaved amplified polymorphic sequence markers developed based on the single-nucleotide polymorphisms from the ICY/CY-specific region, confirmed that C2 is located on Chr. 2 and cosegregated with the marker (M7) derived from a non-synonymous single-nucleotide polymorphism of the pentatricopeptide repeat (PPR) gene (ClPPR, Cla97C02G039880). Additionally, 27 watermelon inbred lines of ICY, CY, and red flesh were evaluated using previously reported Cllcyb (C locus)-based markers and our C2 locus-linked ClPPR-based marker (M7). As a result, dominant alleles at the C2 locus were required to produce CY, in addition to dominant alleles at the C locus, while a recessive homozygous genotype at the C locus gave the red flesh irrespective of the genotype at the C2 locus. Using a ClPPR-based cleaved amplified polymorphic sequence developed in this study and Cllcyb-based markers, watermelon cultivars with CY, ICY, and red flesh could be successfully discerned, implying that the combined use of these markers will be efficient for marker-assisted selection of flesh color in watermelon breeding.https://www.frontiersin.org/articles/10.3389/fgene.2023.1256627/fullCitrullus lanatusflesh colorpentatricopeptide repeatmarker-assisted selectionwatermelon |
| spellingShingle | Girim Park Durre Shahwar Gaeun Jang Jagyeong Shin Gibeom Kwon Younjae Kim Chang Oh Hong Chang Oh Hong Bingkui Jin Hoytaek Kim Oakjin Lee Younghoon Park Younghoon Park Identification of a novel locus C2 controlling canary yellow flesh color in watermelons Frontiers in Genetics Citrullus lanatus flesh color pentatricopeptide repeat marker-assisted selection watermelon |
| title | Identification of a novel locus C2 controlling canary yellow flesh color in watermelons |
| title_full | Identification of a novel locus C2 controlling canary yellow flesh color in watermelons |
| title_fullStr | Identification of a novel locus C2 controlling canary yellow flesh color in watermelons |
| title_full_unstemmed | Identification of a novel locus C2 controlling canary yellow flesh color in watermelons |
| title_short | Identification of a novel locus C2 controlling canary yellow flesh color in watermelons |
| title_sort | identification of a novel locus c2 controlling canary yellow flesh color in watermelons |
| topic | Citrullus lanatus flesh color pentatricopeptide repeat marker-assisted selection watermelon |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1256627/full |
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